EXPLORING THE ROLE OF ANKRD1 IN BREAST INVASIVE CARCINOMA: EXPRESSION ANALYSIS, PROMOTER METHYLATION, AND PROGNOSTIC IMPLICATIONS THROUGH BIOINFORMATICS ANALYSIS
Main Article Content
Keywords
ANKRD1, Cancer, Biomarker, Prognosis
Abstract
The present study deciphers the expression patterns and methylation status of ANKRD1, a potential biomarker, in breast invasive carcinoma (BRCA) using bioinformatics tools. Initially, ANKRD1 expression was analyzed in BRCA and normal control samples, revealing significant down-regulation in tumor samples compared to controls, indicating its potential role in BRCA progression. Subsequently, ANKRD1 expression was assessed across various clinical parameters, including cancer stages, patient demographics, and race. Notable discrepancies in ANKRD1 expression were observed across different cancer stages, genders, ages, and racial backgrounds of BRCA patients, emphasizing its clinical significance in BRCA pathogenesis. Promoter methylation analysis revealed significant hypo-methylation of ANKRD1 in BRCA samples compared to normal controls, suggesting potential epigenetic regulation of ANKRD1 expression in BRCA. Furthermore, survival analysis using the KM plotter tool demonstrated a significant correlation between ANKRD1 expression levels and patient survival outcomes, with high ANKRD1 expression associated with poor overall survival in BRCA patients. Mutational analysis revealed minimal genetic alterations in ANKRD1 in BRCA samples, suggesting that genetic variations have limited impact on ANKRD1 dysregulation in BRCA. These findings underscore the potential of ANKRD1 as a prognostic marker and therapeutic target in BRCA management.
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Muhammad Ejaz
Department of Zoology, Government FMF Graduate College, Gujranwala - Pakistan
Muhammad Jamil Yousaf
Department of Zoology, Government Graduate College Satellite Town, Gujranwala - Pakistan
Hafeez Ullah
Department of Microbiology, Abasyn University, Peshawar - Pakistan
Asma Iqbal
Department of Zoology, University of the Punjab, Lahore - Pakistan
Ahad Masood Hashmi
Department of Zoology, University of Central Punjab, Gujrat - Pakistan
Junaid Farooq
Institute of Biotechnology and Microbiology, Bacha Khan University, Charsadda - Pakistan
Usama Tahir
Department of Microbiology, Sarhad Government College University Faisalabad, Faisalabad - Pakistan
Abdul Waheed Khan
Institute of Biotechnology and Microbiology, Bacha Khan University, Charsadda - Pakistan
Inam Ullah Khan
Department of Microbiology, Sarhad University of Sciences & Information Technology, Peshawar - Pakistan
Syed Meesam Raza
Department of Microbiology, Quaid I Azam University, Islamabad - Pakistan
Jamal Muhammad Khan
Department of Parasitology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur - Pakistan