ILLUMINATING THE ROLE OF TP53 IN ACUTE LYMPHOBLASTIC LEUKEMIA (ALL) USING INTEGRATED BIOINFORMATICS METHODOLOGY
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Keywords
TP53, ALL, Cancer, Biomarker, Treatment
Abstract
In this study, we investigated the expression pattern, promoter methylation pattern, and diagnostic significance of TP53 in acute lymphoblastic leukemia (ALL) through a multi-faceted bioinformatics approach. Utilizing the GSE48558 dataset from the GEO database, we observed a significant down-regulation of TP53 mRNA expression in ALL samples compared to normal controls (P value < 0.05). ROC curve analysis further demonstrated TP53's robust discriminatory power, highlighting its promise as a diagnostic marker for ALL. Subsequent investigation using the UALCAN database revealed consistent TP53 expression patterns across different races and genders of ALL patients, with no significant differences observed (P values > 0.05). Additionally, promoter methylation analysis indicated a slight elevation in TP53 promoter methylation levels in African-American and Asian ALL patients compared to Caucasians, although these differences were not significant (P value > 0.05). Survival analysis using the KM plotter tool revealed that low TP53 expression correlated with poorer overall survival (OS) among ALL patients (P value < 0.05), suggesting its potential as a prognostic biomarker. Our findings underscore the diagnostic and prognostic significance of TP53 in ALL, providing valuable insights for future research and clinical interventions. These results emphasize the importance of TP53 in guiding therapeutic strategies and improving outcomes for ALL patients.
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Shahid Ullah
Department of Biochemistry Abdul Wali Khan University, Mardan, Pakistan.
Alex Tonks
Department of Haematology, Division of Cancer & Genetics, School of Medicine Cardiff University United Kingdom.
Asif Ullah Khan
Department of Biochemistry Abdul Wali Khan University, Mardan, Pakistan.
Abdulsalam M Alruwaili
Department of Haematology, Division of Cancer & Genetics, School of Medicine Cardiff University United Kingdom.
Hedib Alkoumi H Alrawili
Wolfson College, School of Medicine, Oxford University, England UK.
Carlos Eliel Maya-Ramírez
Laboratorio de Diagnóstico molecular, Departamento de Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala S/N, Colonia, Casco de Santo Tomás, C.P.,11340 Ciudad de México, México.
Jamal Muhammad Khan
Department of Parasitology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan.
Muhammad Arif Lodhi
Department of Biochemistry Abdul Wali Khan University, Mardan, Pakistan.