THE DYSREGULATED EXPRESSION OF THE P53 GENE IS ASSOCIATED WITH POOR PROGNOSIS IN CEBPA MUTANT ACUTE MYELOID LEUKEMIA PATIENTS
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Keywords
Acute myeloid leukemia, p53 gene, gene expression
Abstract
The main objective of the current investigation is to examine the influence of altered p53 gene expression on the prognosis in CEBPA mutant acute myeloid leukemia (AML) patients. AML is a heterogeneous disorder that arises due to acquired genetic anomalies such as CEBPA and TP53. The CEBPA transcription factor plays a critical role in the differentiation of specific blood cells, while the p53 gene functions as a protector of the genome, preventing the development of tumors and regulating various genes involved in cell cycle arrest, DNA repair and programmed cell death. Nevertheless, there is a paucity of comprehensive data concerning the evaluation of p53 expression, which is the resultant of the p53 gene, particularly in hematologic malignancies. In this research investigation, a cohort of thirty individuals diagnosed with AML and twenty-five healthy control participants, who granted informed consent, were enrolled. The evaluation of p53 expression levels was carried out through quantitative Real-time PCR (qRT-PCR). Statistical assessments were performed utilizing one-way ANOVA and Tukey test, facilitated by GraphPad Prism software version 9.0.0. The p53wt/CEBPAmut group (0.20 ± 0.032 SEM) and p53mut/CEBPAmut group (0.06 ± 0.011 SEM) demonstrated a significant decrease in p53 expression compared to the control group (1.00 ± 0.010 SEM) with statistical significance (p<0.0001) for both comparisons respectively. Furthermore, the p53mut/CEBPAmut group exhibited a significantly lower level of p53 expression (p<0.01) when contrasted with the p53wt/CEBPAmut group. In relation to both the overall survival and event-free survival results, a univariate analysis indicated a statistically significant variance in overall survival (p=0.0299, HR = 3.401) and event-free survival (p=0.0128, HR = 3.002) between individuals with p53wt/CEBPAmut and p53mut/CEBPAmut.
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Muhammad Husain
Institute of Zoology, University of the Punjab, Lahore, Pakistan
Ayesha Aihetasham
Institute of Zoology, University of the Punjab, Lahore, Pakistan
Afia Muhammad Akram
Department of Zoology, University of Education, Lahore, Pakistan.
Sabrina waheed
Institute of Zoology, University of the Punjab, Lahore, Pakistan
Rafia Tabassum
Institute of Zoology, University of the Punjab, Lahore, Pakistan
Salma Ashraf
Institute of Zoology, University of the Punjab, Lahore, Pakistan
Asma Iqbal
Institute of Zoology, University of the Punjab, Lahore, Pakistan
Syed Shamsheer Abbas Ali Shah
Institute of Zoology, University of the Punjab, Lahore, Pakistan
Anam Mobeen
Institute of Zoology, University of the Punjab, Lahore, Pakistan
Ghulam Fatima Saleem
Institute of Zoology, University of the Punjab, Lahore, Pakistan