Evaluation Of Antimicrobial And Cytotoxic Effects Of 1,25 Dihydroxycholecalciferol Formulation- An Invitro Study

Main Article Content

Sandra Sagar
Pratibha Ramani
Rajeshkumar.S
Abilasha.R

Keywords

1,25 dihydroxycholecalciferol, vitamin D, formulation, antioxidant, anti-inflammatory

Abstract

Background: Vitamin D is a fat-soluble pro-hormone that can be obtained through exposure to the sun or derived from ingested foods or supplements. It is available in two forms: vitamin D2, also known as ergocalciferol, and vitamin D3, also known as cholecalciferol. It is important to remember, however, that many individuals may have inadequate exposure to UV light due to lack of outdoor activity and the use of protective sunscreen lotions and clothing
Aim: To prepare a formulation containing 1,25 dihydroxycholecalciferol. To determine the antimicrobial and cytotoxic effects of the formulation containing 1,25 dihydroxycholecalciferol .
Materials And Methods: In the present study, preparation of the formulation was carried out initially and then the antimicrobial and cytotoxic activity of the formulation was assessed. The antimicrobial activity of the mouthwash formulation was carried out using agar well diffusion method. Different concentrations of nanoparticles were tested against Streptococcus mutans, E.faecalis lactobacillus and Candida albicans. The cytoxicity activity of the mouthwash was assessed using brine shrimp lethality assay method.
Results: The cytotoxic activity was found to be better at low concentration.8 nauplii survived at 5 μL, 10 μL, 20 μL after 24 hours.The antimicrobial activity of the mouthwash formulation showed that the mouthwash formulation had the highest zone of inhibhition seen in 100 μL concentration on C.albicans almost similar to the contrl antibiotic in comparison to the oral pathogens.
Discussion:1,25 dihydroxycholecalciferol has many medicinal properties. Our study has evaluated the concentration of the formulation and has found positive results.
Conclusion: The prepared formulation had better antimicrobial activity and exhibhited less cytotxicity at lower concentrations.

Abstract 130 | PDF Downloads 123

References

1. Holick MF, Binkley NC, Bischoff-Ferrari HA, Gordon CM,Hanley DA, Heaney RP,et al. Evaluation, Treatment, and Prevention of Vitamin D Deficiency: an Endocrine Society Clinical Practice Guideline. J Clin Endocrinol Metab 2011; 96 (7):1911–30.
2. Dietary Reference Intakes for Calcium and Vitamin D. 2011 Washington, D.C.: National Academies Press;2011
3. Pepper KJ, Judd SE, Nanes MS, Tangpricha V. Evaluation of vitamin D repletion regimens to correct vitamin D status in adults. Endocr Pract 2009;15 (2) :95–103.
4. Cannell JJ, Hollis BW. Use of vitamin D in clinical practice. Altern Med Rev 2008;13 (1):6–20.
5. Matthana MH. The relation between vitamin D deficiency and fibromyalgia syndrome in women. Saudi Med J 2011;32(9):925–9.
6. Harvey NC, Holroyd C, Ntani G, Javaid K, Cooper P,MoonR,et al. Vitamin D supplementation in pregnancy: a systematic review. Health Technol Assess2014;18 (45):1–190.
7. Sahay M, Sahay R. Rickets–vitamin D deficiency and dependency. Indian J Endocrinol Metab 2012;16(2) :164–76.
8. Confino-Cohen R, Brufman I, Goldberg A,Feldman BS. Vitamin D, asthma prevalence and asthma exacerbations: a large adult population-based study. Allergy 2014;69 (12):1673–80.
9. Institute of Medicine, Food and Nutrition Board. Dietary Reference Intakes for Calcium and Vitamin D. Washington, DC: National Academy Press, 2010.
10. Norman AW, Henry HH. Vitamin D. In: Erdman JW, Macdonald IA, Zeisel SH, eds. Present Knowledge in Nutrition, 10th ed. Washington DC: Wiley-Blackwell, 2012.
11. Jones G. Vitamin D. In: Ross AC, Caballero B, Cousins RJ, Tucker KL, Ziegler TR, eds. Modern Nutrition in Health and Disease, 11th ed. Philadelphia: Lippincott Williams & Wilkins, 2014.
12. Li, Zhenjiang, Vishnu Priya Veeraraghavan, Surapaneni Krishna Mohan, Srinivasa Rao Bolla, Hariprasath Lakshmanan, Subramanian Kumaran, Wilson Aruni, et al. 2020. “Apoptotic Induction and Anti-Metastatic Activity of Eugenol Encapsulated Chitosan Nanopolymer on Rat Glioma C6 Cells via Alleviating the MMP Signaling Pathway.” Journal of Photochemistry and Photobiology B: Biology. https://doi.org/10.1016/j.jphotobiol.2019.111773.
13. Markov, Alexander, Lakshmi Thangavelu, Surendar Aravindhan, Angelina Olegovna Zekiy, Mostafa Jarahian, Max Stanley Chartrand, Yashwant Pathak, Faroogh Marofi, Somayeh Shamlou, and Ali Hassanzadeh. 2021. “Mesenchymal Stem/stromal Cells as a Valuable Source for the Treatment of Immune-Mediated Disorders.” Stem Cell Research & Therapy 12 (1): 192.
14. Mohan, Meenakshi, and Nithya Jagannathan. 2014. “Oral Field Cancerization: An Update on Current Concepts.” Oncology Reviews 8 (1): 244.
15. Neelakantan, Prasanna, Deeksha Grotra, and Subash Sharma. 2013. “Retreatability of 2 Mineral Trioxide Aggregate-Based Root Canal Sealers: A Cone-Beam Computed Tomography Analysis.” Journal of Endodontia 39 (7): 893–96.
16. Paramasivam, Arumugam, Jayaseelan Vijayashree Priyadharsini, Subramanian Raghunandhakumar, and Perumal Elumalai. 2020. “A Novel COVID-19 and Its Effects on Cardiovascular Disease.” Hypertension Research: Official Journal of the Japanese Society of Hypertension.
17. Sheriff, K. Ahmed Hilal, K. Ahmed Hilal Sheriff, and Archana Santhanam. 2018. “Knowledge and Awareness towards Oral Biopsy among Students of Saveetha Dental College.” Research Journal of Pharmacy and Technology. https://doi.org/10.5958/0974-360x.2018.00101.4.

Most read articles by the same author(s)

1 2 3 4 > >>