Embryonic toxicology and antimicrobial potential of strontium nanoparticles synthesized using Oolong tea

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Sandhya Sundar
Rajesh kumar Shanmugam
Ramya Ramadoss
Suganya Paneerselvem
Pratibha Ramani


oolong tea, strontium nanoparticles,zebrafish, Embryonic toxicity, antimicrobial potential


Background: Oolong tea due to the presence of flavonoids such as catechins, theaflavins, and TheaSinensis is used as a reducing agent to prepare strontium nanoparticles. When developing metal nanoparticles, the plant extracts-mediated technique is very effective and advantageous compared to employing other techniques.
Aim: To synthesize and evaluate the cytotoxicity, embryonic toxicology and antimicrobial properties of strontium nanoparticles mediated through oolong tea.
Materials and methods: Oolong tea extract was prepared and added with Strontium oxide to synthesize the strontium nanoparticles. The embryonic toxicity of the prepared nanoparticles is determined by assessing the toxicity to the hatching rate and viability rate of zebrafish embryos in different concentrations . The antimicrobial potential of the produced nanoparticles were assessed by determining the zone of inhibition against the common oral pathogens such as staph.aureus, strep.mutans, E.faecalis, candida. Albicans.
Result: There was also concentration dependent alterations in the hatching and viability rate of zebrafish. The anti-microbial assessment demonstrated the mean zone of inhibition of 23mm against Enterococcus faecalis, 33mm against Staphylococcus aureus, 23mm against Streptococcus mutans and 9mm against Candida albicans, by the end of fourth day. Conclusion: Oolong tea shows strong antimicrobial activity when treated with strontium nanoparticles thus it can find a great application in preventive dentistry. 

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1. Joseph Needham et al, science and civilisation in china, vol 6, Cambridge university press,2000,Tea processing and use
2. Smith Krisi et al,World atlas of Tea,2016
3. A. Bakhtiar et al, pH responsive strontium nanoparticles for targeted gene therapy against mammary carcinoma cells,2021
4. JIS Z 2801:2000 Antimicrobial products-test for antimicrobial activity and efficacy,200)
5. Cela P. ,Embryonic toxicity of nanoparticles,2014
6. Arun K Thalla et al, Green synthesis of nanoparticles, 2018
7. Dasari et al, green synthesis, characterisation and antimicrobial activity of silver nanoparticles using leaf extracts of carissa carandas
8. CC Chou et al,Antimicrobial activity of tea as affected by degree of fermentation and manufacturing season,1999
9. SC Wu et al, Antimutagenic and antimicrobial activity of pu-erh tea,2007
10. H Zhang et al, The impact of oolong and black tea polyphenols on human health
11. Thangavelu L et al, Role of Nanoparticles in Environmental Remediation: An Insight into Heavy Metal Pollution from Dentistry. Bioinorg Chem Appl. 2022 Mar 17;2022:1946724. (22)
12. Godlin Genita et al, comparative evaluation of chlorhexidine, aloe vera and green tea,2017.
13. Andhare, Mangesh G., Sunaina Shetty, Gopinath Vivekanandan, Raghavendra M. Shetty, Betul Rahman, Shishir R. Shetty, Srinivasa T. Siddeshappa, and Vijay Desai. 2022. “Clinical Efficacy of Green Tea, Aloe Vera and Chlorhexidine Mouthwashes in the Treatment of Dental Biofilm Induced Gingivitis: A Multi-Arm, Double-Blinded, Randomized Controlled Clinical Trial.” International Journal of Dental Hygiene, December. https://doi.org/10.1111/idh.12664.
14. Chou, C. C., L. L. Lin, and K. T. Chung. 1999. “Antimicrobial Activity of Tea as Affected by the Degree of Fermentation and Manufacturing Season.” International Journal of Food Microbiology 48 (2): 125–30.
15. Committee, E35, and E35 Committee. n.d. “Test Method for Production of Clostridium Difficile Spores for Use in Efficacy Evaluation of Antimicrobial Agents.” https://doi.org/10.1520/e2839.
16. Dasari, Prabhakara Rao, and Prashant Anthony. 2017. “Green Synthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles Using Leaf Extracts of Carissa Carandas.” Asian Journal of Chemistry. https://doi.org/10.14233/ajchem.2017.20713.
17. Devatha, Chella Purushothaman, and Arun K. Thalla. 2018. “Green Synthesis of Nanomaterials.” Synthesis of Inorganic Nanomaterials. https://doi.org/10.1016/b978-0-08-101975-7.00007-5.
18. Karim, Md Emranul, Sheikh Tanzina Haque, Hamed Al-Busaidi, Athirah Bakhtiar, Kyi Kyi Tha, Mark M. Banaszak Holl, and Ezharul Hoque Chowdhury. 2022. “Scope and Challenges of Nanoparticle-Based mRNA Delivery in Cancer Treatment.” Archives of Pharmacal Research 45 (12): 865–93.
19. Needham, Joseph, and Colin A. Ronan. 1978. The Shorter Science and Civilisation in China: Volume 5. Cambridge University Press.
20. Smith, Krisi. 2016. World Atlas of Tea: From the Leaf to the Cup, the World’s Teas Explored and Enjoyed. Hachette UK.
21. Thangavelu, Lakshmi, Geetha Royapuram Veeraragavan, Sreekanth Kumar Mallineni, Ezhilarasan Devaraj, Royapuram Parthasarathy Parameswari, Nazmul Huda Syed, Kamal Dua, Dinesh Kumar Chellappan, Sri Renukadevi Balusamy, and Ujjal K. Bhawal. 2022. “Role of Nanoparticles in Environmental Remediation: An Insight into Heavy Metal Pollution from Dentistry.” Bioinorganic Chemistry and Applications 2022 (March): 1946724.
22. Wu, She-Ching, Gow-Chin Yen, Bor-Sen Wang, Chih-Kwang Chiu, Wen-Jye Yen, Lee-Wen
Chang, and Pin-Der Duh. 2007. “Antimutagenic and Antimicrobial Activities of Pu-Erh Tea.” LWT - Food Science and Technology. https://doi.org/10.1016/j.lwt.2005.11.008.
23. Zhang, Hua, Ruili Qi, and Yoshinori Mine. 2019. “The Impact of Oolong and Black Tea Polyphenols on Human Health.” Food Bioscience. https://doi.org/10.1016/j.fbio.2019.03.009.

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