Anticancer activity of Green synthesized selenium nanoparticles from Garcinia Mangostana Crude extract against MCF-7 Breast cancer cells
Main Article Content
Keywords
garcinia mangostana, selenium nanoparticle, MTT assay, antitumor activity, green synthesis
Abstract
Background: Nano-biotechnology has evolved and plays a crucial role in the detection and treatment of many diseases in the current scenario. An eco-friendly and efficient method (photoinduced) has been used for green synthesis of stable selenium nanoparticles (SeNPs) using aqueous extract as a reducing and stabilizing agent, derived from fruit extract of garcinia mangostana.
Aim: The aim of this study was to evaluate the anticancer activity of green synthesized GM selenium nanoparticles (Se-NPs) against breast cancer cells (MCF-7).
Methods And Materials: The preparation of selenium-induced Garcinia Mangostana nanoparticles were examined by ultraviolet-visible spectroscopy with a wavelength of range from 25-650nm. The selenium nanoparticle pellet was stored in sealed vials after being calcined for two hours at 70°C in a hot air oven.In-vitro antitumor activity of Se-NPs was evaluated by MTT assay. Inverted microscope was used to evaluate the morphology of the cells.
Results: The synthesized Selenium Nanoparticles have anticancer against MCF-7 cell lines. The cytotoxicity study exhibited a dose-dependent effect against breast cancer cells (MCF-7) using MTT assay, the inhibitory concentration (IC50) was found to be 50μg/mL . Inverted phase contrast microscope showed low viability of cell at a concentration of 60ul in the breast cancer cell line.
Conclusion: In conclusion, the results of this study demonstrated that Garcinia Mangostana induced selenium Nanoparticles extract may be a potential therapeutic agent for human breast cancer treatment.
References
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35. McDougall GJ, Ross HA, Ikeji M, Stewart D. Berry Extracts Exert Different Antiproliferative Effects against Cervical and Colon Cancer Cells Grown in Vitro [Internet]. Vol. 56, Journal of Agricultural and Food Chemistry. 2008. p. 3016–23. Available from: http://dx.doi.org/10.1021/jf073469n
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2. Teixidó J, Hidalgo A, Fagerholm SC. Leukocyte Trafficking in Homeostasis and Disease. Frontiers Media SA; 2019. 153 p.
3. Pathak YV. Surface Modification of Nanoparticles for Targeted Drug Delivery. Springer; 2019. 499 p.
4. Bhat MA, Wani IA, Ashraf S. Applications of Nanomaterials in Agriculture, Food Science, and Medicine. IGI Global; 2020. 442 p.
5. Kesharwani P, Taurin S, Greish K. Theory and Applications of Nonparenteral Nanomedicines. Academic Press; 2020. 540 p.
6. Malik A, Afaq S, Tarique M. Nanomedicine for Cancer Diagnosis and Therapy. Springer Nature; 2021. 249 p.
7. Mathew MG, Samuel SR, Soni AJ, Roopa KB. Evaluation of adhesion of Streptococcus mutans, plaque accumulation on zirconia and stainless steel crowns, and surrounding gingival inflammation in primary molars: randomized controlled trial. Clin Oral Investig. 2020 Sep;24(9):3275–80.
8. Princeton B, Santhakumar P, Prathap L. Awareness on Preventive Measures taken by Health Care Professionals Attending COVID-19 Patients among Dental Students. Eur J Dent. 2020 Dec;14(S 01):S105–9.
9. Rayman MP. Selenium and human health [Internet]. Vol. 379, The Lancet. 2012. p. 1256–68. Available from: http://dx.doi.org/10.1016/s0140-6736(11)61452-9
10. Tugarova AV, Mamchenkova PV, Dyatlova YA, Kamnev AA. FTIR and Raman spectroscopic studies of selenium nanoparticles synthesised by the bacterium Azospirillum thiophilum [Internet]. Vol. 192, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2018. p. 458–63. Available from: http://dx.doi.org/10.1016/j.saa.2017.11.050
11. Winkler HC, Suter M, Naegeli H. Critical review of the safety assessment of nano-structured silica additives in food [Internet]. Vol. 14, Journal of Nanobiotechnology. 2016. Available from: http://dx.doi.org/10.1186/s12951-016-0189-6
12. Yadav AK, Gupta U, Sharma R. Nano Drug Delivery Strategies for the Treatment of Cancers. Academic Press; 2020. 374 p.
13. Zhou Y, Xu M, Liu Y, Bai Y, Deng Y, Liu J, et al. Green synthesis of Se/Ru alloy nanoparticles using gallic acid and evaluation of theiranti-invasive effects in HeLa cells [Internet]. Vol. 144, Colloids and Surfaces B: Biointerfaces. 2016. p. 118–24. Available from: http://dx.doi.org/10.1016/j.colsurfb.2016.04.004
14. Wang X, Sun K, Tan Y, Wu S, Zhang J. Efficacy and safety of selenium nanoparticles administered intraperitoneally for the prevention of growth of cancer cells in the peritoneal cavity. Free Radic Biol Med. 2014 Jul;72:1–10.
15. Rayman MP. Selenium and human health [Internet]. Vol. 379, The Lancet. 2012. p. 1256–68. Available from: http://dx.doi.org/10.1016/s0140-6736(11)61452-9
16. Sanmartín C, Plano D, Sharma AK, Palop JA. Selenium Compounds, Apoptosis and Other Types of Cell Death: An Overview for Cancer Therapy [Internet]. Vol. 13, International Journal of Molecular Sciences. 2012. p. 9649–72. Available from: http://dx.doi.org/10.3390/ijms13089649
17. Website [Internet]. Available from: https://scholar.google.co.in/scholar?q=L.+Patrick,+Selenium+biochemistry+and+cancer:+A+review+of+the+literature.+Alternative+Medicine+Review&hl=en&as_sdt=0&as_vis=1&oi=scholart
18. Fazal, S., Jayasree, A., Sasidharan, S., Koyakutty, M., Nair, S. V., & Menon, D. (2014). Green synthesis of anisotropic gold nanoparti- cles for photothermal therapy of cancer. ACS Applied Materials & Interfaces, 6(11), 8080–8089. https://doi.org/10.1021/am500302t.
19. Genevieve, A., Kahrilas Laura, M., Wally, S. J., Fredrick Michael, H., Amy, L., Prieto, J. E., & Owens. (2014). Microwave-assisted green synthesis of silver nanoparticles using orange peel extract. ACS Sustainable Chemistry & Engineering, 2(3), 367–376. https://doi.org/10.1021/sc4003664.
20. Ramamurthy, C. H., Sampath, K. S., Arunkumar, P., Suresh kumar, M., Sujatha, V., Premkumar, K., & Thirunavukkarasu, C. (2013). Green synthesis and characterization of selenium nanoparticles and its augmented cytotoxicity with doxorubicin on cancer cells. Bioprocess and Biosystems Engineering, 36(8), 1131–1139. https://doi.org/10.1007/s00449-012-0867-1.
21. Suresh D, Nethravathi PC, Udayabhanu, Rajanaika H, Nagabhushana H, Sharma SC. Green synthesis of multifunctional zinc oxide (ZnO) nanoparticles using Cassia fistula plant extract and their photodegradative, antioxidant and antibacterial activities [Internet]. Vol. 31, Materials Science in Semiconductor Processing. 2015. p. 446–54. Available from: http://dx.doi.org/10.1016/j.mssp.2014.12.023
22. Raj Preeth D, Saravanan S, Shairam M, Selvakumar N, Selestin Raja I, Dhanasekaran A, et al. Bioactive Zinc(II) complex incorporated PCL/gelatin electrospun nanofiber enhanced bone tissue regeneration. Eur J Pharm Sci. 2021 May 1;160:105768.
23. Bañuelos G, Lin ZQ, Liang D, Yin XB. Selenium Research for Environment and Human Health: Perspectives, Technologies and Advancements: Proceedings of the 6th International Conference on Selenium in the Environment and Human Health (ICSEHH 2019), October 27-30, 2019, Yangling, Xi’an, China. CRC Press; 2019. 228 p.
24. Bajpai S, Shukla PS, Adil M, Asiedu S, Pruski K, Prithiviraj B. First Report of Bacterial Leaf Blight of Strawberry Caused by Pantoea ananatis in Nova Scotia, Canada [Internet]. Vol. 104, Plant Disease. 2020. p. 276–276. Available from: http://dx.doi.org/10.1094/pdis-05-19-1042-pdn
25. Prithiviraj M, Muralikannan R. Investigation of Optimal Alkali-treated Perotis indica Plant Fibers on Physical, Chemical, and Morphological Properties [Internet]. Vol. 19, Journal of Natural Fibers. 2022. p. 2730–43. Available from: http://dx.doi.org/10.1080/15440478.2020.1821291
26. Moongkarndi P, Kosem N, Kaslungka S, Luanratana O, Pongpan N, Neungton N. Antiproliferation, antioxidation and induction of apoptosis by Garcinia mangostana (mangosteen) on SKBR3 human breast cancer cell line. J Ethnopharmacol. 2004 Jan;90(1):161–6.
27. Kalick LS, Khan HA, Maung E, Baez Y, Atkinson AN, Wallace CE, et al. Mangosteen for malignancy prevention and intervention: Current evidence, molecular mechanisms, and future perspectives. Pharmacol Res. 2022 Dec 26;188:106630.
28. Sharma G, Sharma A, Bhavesh R, Park J, Ganbold B, Nam JS, et al. Biomolecule-Mediated Synthesis of Selenium Nanoparticles using Dried Vitis vinifera (Raisin) Extract [Internet]. Vol. 19, Molecules. 2014. p. 2761–70. Available from: http://dx.doi.org/10.3390/molecules19032761
29. Angamuthu A, Venkidusamy K, Muthuswami RR. Synthesis and Characterization of Nano-selenium and its Antibacterial Response on Some Important Human Pathogens [Internet]. Vol. 116, Current Science. 2019. p. 285. Available from: http://dx.doi.org/10.18520/cs/v116/i2/285-290
30. Synthesis of Monodisperse Selenium Nanospheres and Self-assembled Monolayers Using Poly(vinyl pyrrolidone) as Dual Reductant/colloid Stabilizer [Internet]. Vol. 29, Bulletin of the Korean Chemical Society. 2008. p. 689–92. Available from: http://dx.doi.org/10.5012/bkcs.2008.29.3.689 31. Chen T, Wong YS, Zheng W, Bai Y, Huang L. Selenium nanoparticles fabricated in Undaria pinnatifida polysaccharide solutions induce mitochondria-mediated apoptosis in A375 human melanoma cells [Internet]. Vol. 67, Colloids and Surfaces B: Biointerfaces. 2008. p. 26–31. Available from: http://dx.doi.org/10.1016/j.colsurfb.2008.07.010
32. Akao Y, Nakagawa Y, Nozawa Y. Anti-cancer effects of xanthones from pericarps of mangosteen. Int J Mol Sci. 2008 Mar;9(3):355–70.
33. Robotin MC, Kansil M, Howard K, George J, Tipper S, Dore GJ, et al. Antiviral therapy for hepatitis B-related liver cancer prevention is more cost-effective than cancer screening [Internet]. Vol. 50, Journal of Hepatology. 2009. p. 990–8. Available from: http://dx.doi.org/10.1016/j.jhep.2008.12.022
34. Nauman MC, Johnson JJ. The purple mangosteen (Garcinia mangostana): Defining the anticancer potential of selected xanthones. Pharmacol Res. 2022 Jan;175:106032.
35. McDougall GJ, Ross HA, Ikeji M, Stewart D. Berry Extracts Exert Different Antiproliferative Effects against Cervical and Colon Cancer Cells Grown in Vitro [Internet]. Vol. 56, Journal of Agricultural and Food Chemistry. 2008. p. 3016–23. Available from: http://dx.doi.org/10.1021/jf073469n
36. Wei T, Chen C, Hou J, Xin W, Mori A. Nitric oxide induces oxidative stress and apoptosis in neuronal cells [Internet]. Vol. 1498, Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 2000. p. 72–9. Available from: http://dx.doi.org/10.1016/s0167-4889(00)00078-1
37. Menasria F, Azebaze AGB, Billard C, Faussat AM, Nkengfack AE, Meyer M, et al. Apoptotic effects on B-cell chronic lymphocytic leukemia (B-CLL) cells of heterocyclic compounds isolated from Guttiferaes. Leuk Res. 2008 Dec;32(12):1914–26.
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Apr 11, 2023
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Georgia BenithaJ, Gheena S, Pratibha Ramani, Rajesh Kumar S, Kartikeyan Ramalingam, & Abilasha Ramasubramaniam. (2023). Anticancer activity of Green synthesized selenium nanoparticles from Garcinia Mangostana Crude extract against MCF-7 Breast cancer cells. Journal of Population Therapeutics and Clinical Pharmacology, 30(6), 74–82. https://doi.org/10.47750/jptcp.2023.30.06.011
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