Anticancer activity of Green synthesized selenium nanoparticles from Garcinia Mangostana Crude extract against MCF-7 Breast cancer cells

Main Article Content

Georgia BenithaJ
Gheena S
Pratibha Ramani
Rajesh Kumar S
Kartikeyan Ramalingam
Abilasha Ramasubramaniam

Keywords

garcinia mangostana, selenium nanoparticle, MTT assay, antitumor activity, green synthesis

Abstract

Background: Nano-biotechnology has evolved and plays a crucial role in the detection and treatment of many diseases in the current scenario. An eco-friendly and efficient method (photoinduced) has been used for green synthesis of stable selenium nanoparticles (SeNPs) using aqueous extract as a reducing and stabilizing agent, derived from fruit extract of garcinia mangostana.
Aim: The aim of this study was to evaluate the anticancer activity of green synthesized GM selenium nanoparticles (Se-NPs) against breast cancer cells (MCF-7).
Methods And Materials: The preparation of selenium-induced Garcinia Mangostana nanoparticles were examined by ultraviolet-visible spectroscopy with a wavelength of range from 25-650nm. The selenium nanoparticle pellet was stored in sealed vials after being calcined for two hours at 70°C in a hot air oven.In-vitro antitumor activity of Se-NPs was evaluated by MTT assay. Inverted microscope was used to evaluate the morphology of the cells.
Results: The synthesized Selenium Nanoparticles have anticancer against MCF-7 cell lines. The cytotoxicity study exhibited a dose-dependent effect against breast cancer cells (MCF-7) using MTT assay, the inhibitory concentration (IC50) was found to be 50μg/mL . Inverted phase contrast microscope showed low viability of cell at a concentration of 60ul in the breast cancer cell line.
Conclusion: In conclusion, the results of this study demonstrated that Garcinia Mangostana induced selenium Nanoparticles extract may be a potential therapeutic agent for human breast cancer treatment.

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