Anti-inflammatory activity of Euphorbia Tirucalli Mediated Selenium nanoparticles: An in vitro study
Main Article Content
Keywords
Anti-inflammatory activity, Aqueous extract of Euphorbia Tirucalli, Selenium Nanoparticle
Abstract
Nanomaterials have become a promising commodity in numerous industries during the past decade, including cosmetics, healthcare, biomedicine, and food. Selenium, a "drug nanocarrier," has potent antibacterial, antioxidant, anti-cancer, and anti-inflammatory effects. This study is aimed at synthesizing selenium nanoparticles using Euphorbia tirucalli to characterize and evaluate the anti-inflammatory activity.
Aim: This study aims to evaluate the anti-inflammatory activity of the Euphorbia tirucalli stem extract mediated by selenium nanoparticles.
Materials and methods: E.tirucalli extract was prepared. The biosynthesis of selenium nanoparticles was measured using UV vis spectrometry at wavelengths of 300-600 nm and the anti-inflammatory activity was determined using Bovine Serum Albumin at 10, 20, 30, 40, and 50 μl.
Results: The inhibition percentage was maximum at 81.2 % at IC50 concentration. The percentage of inhibitions was higher than the standard values in all the concentrations.
Conclusion: Selenium nanoparticles induced aqueous stem extract of Euphorbia tirucalli showed a higher percentage of inhibition when compared to standard. Hence, this can be used in commercial medical products to reduce inflammation and swelling.
References
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21. Sushanthi and Department of Oral and Maxillofacial Surgery, Saveetha Dental College, SIMATS, Saveetha University, Chennai (2021) ‘Vernonia amygdalina mediated copper nanoparticles and its characterization and antimicrobial activity - an in vitro study’, International journal of dentistry and oral science, pp. 3330–3334.
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26. Website (no date e). Available at: https://ui.adsabs.harvard.edu/link_gateway/2019MaTec..34..490T/doi:10.1080/10667857.2019.1583408.
27. Website (no date f). Available at: Prashant Y. Mali, Shital S. Panchal, Euphorbia tirucalli L.: Review on morphology, medicinal uses, phytochemistry and pharmacological activities, Asian Pacific Journal of Tropical Biomedicine, Volume 7, Issue 7, 2017, Pages 603-613, ISSN 2221-1691, https://doi.org/10.1016/j.apjtb.2017.06.002.
28. Website (no date g). Available at: Benjamaa Rania, Moujanni Abdelkarim, Kaushik Neha, Choi Eun Ha, Essamadi Abdel Khalid, Kaushik Nagendra Kumar TITLE=Euphorbia species latex: A comprehensive review on phytochemistry and biological activities JOURNAL=Frontiers in Plant Science VOLUME=13 YEAR=2022 URL=https://www.frontiersin.org/articles/10.3389/fpls.2022.1008881 DOI=10.3389/fpls.2022.1008881.
29. Website (no date h). Available at: Munro, B., Vuong, Q. V., Chalmers, A. C., Goldsmith, C. D., Bowyer, M. C., & Scarlett, C. J. (2015). Phytochemical, Antioxidant and Anti-Cancer Properties of Euphorbia tirucalli Methanolic and Aqueous Extracts. Antioxidants (Basel, Switzerland), 4(4), 647–661. https://doi.org/10.3390/antiox4040647.
30. Website (no date i). Available at: Anu Rana, Krishna Yadav, Sheeja Jagadevan, A comprehensive review on green synthesis of nature-inspired metal nanoparticles: Mechanism, application and toxicity, Journal of Cleaner Production, Volume 272, 2020, 122880, ISSN 0959-6526, https://doi.org/10.1016/j.jclepro.2020.122880. (https://www.sciencedirect.com/science/article/pii/S0959652620329255).
31. Website (no date j). Available at: Sharma, G.; Sharma, A.R.; Bhavesh, R.; Park, J.; Ganbold, B.; Nam, J.-S.; Lee, S.-S. Biomolecule-Mediated Synthesis of Selenium Nanoparticles using Dried Vitis vinifera (Raisin) Extract. Molecules 2014, 19, 2761-2770. https://doi.org/10.3390/molecules19032761.
32. Website (no date k). Available at: El-Batal AI, Zaid OA, Noaman E, Effat SI. In vivo and in vitro antitumor activity of modified citrus pectin in combination with selenium nanoparticles against Ehrlich carcinoma cells. [Last accessed on 2020 Dec];Int Pharm Sci Health Care. 2012 6:23–47. Available from: http://www.rspublication.com/ijphc/index.html . [Google Scholar] [Ref list].
33. Website (no date l). Available at: Menon, S., Ks, S. D., R, S., S, R., & S, V. K. (2018). Selenium nanoparticles: A potent chemotherapeutic agent and an elucidation of its mechanism. Colloids and surfaces. B, Biointerfaces, 170, 280–292. https://doi.org/10.1016/j.colsurfb.2018.06.006.
34. Zambonino, M.C. et al. (2021) ‘Green Synthesis of Selenium and Tellurium Nanoparticles: Current Trends, Biological Properties and Biomedical Applications’, International journal of molecular sciences, 22(3). Available at: https://doi.org/10.3390/ijms22030989.
35. Menon, S., Ks, S. D., R, S., S, R., & S, V. K. (2018). Selenium nanoparticles: A potent chemotherapeutic agent and an elucidation of its mechanism. Colloids and surfaces. B, Biointerfaces, 170, 280–292. https://doi.org/10.1016/j.colsurfb.2018.06.006
2. Antony, J.V.M. et al. (2021) ‘Particle size penetration rate and effects of smoke and smokeless tobacco products - An invitro analysis’, Heliyon, 7(3), p. e06455.
3. Arjunkumar, R. (2018) ‘Nanomaterials for theManagement of Periodontal Diseases’, Dental Applications of Nanotechnology, pp. 203–215.
4. Balasooriya, E.R. et al. (2017) ‘Honey Mediated Green Synthesis of Nanoparticles: New Era of Safe Nanotechnology’, Journal of nanomaterials, 2017. Available at: https://doi.org/10.1155/2017/5919836.
5. Benjamaa, R. et al. (2022) ‘Euphorbia species latex: A comprehensive review on phytochemistry and biological activities’, Frontiers in plant science, 13. Available at: https://doi.org/10.3389/fpls.2022.1008881.
6. Bhonsle, A.S.R. et al. (2021) ‘Anti-inflammatory Activity of Selenium Nanoparticles Synthesised Using Pterocarpus santalinus’, Journal of pharmaceutical research international, pp. 363–371.
7. Binckley, S. and Zahra, F. (2022) ‘Euphorbia Tirucalli’, in StatPearls. Treasure Island (FL): StatPearls Publishing.
8. Francis, T. et al. (2020) ‘Anti-inflammatory and Cytotoxic Effect of Arrow Root Mediated Selenium Nanoparticles’, Pharmacognosy Journal, pp. 1363–1367. Available at: https://doi.org/10.5530/pj.2020.12.188.
9. Jadoun, S. et al. (2020) ‘Green synthesis of nanoparticles using plant extracts: a review’, Environmental chemistry letters, 19(1), pp. 355–374.
10. Kirupagaran*, R., Saritha, A. and Bhuvaneswari, S. (2016) ‘Green Synthesis of Selenium Nanoparticles from Leaf and Stem Extract of Leucas lavandulifolia Sm. and Their Application’, Journal of Nanoscience and Technology, pp. 224–226.
11. Madhumitha, B. et al. (2021) ‘Green Synthesis of Selenium Nanoparticle using Capparis decidua fruit extract and its Characterization using Transmission Electron Microscopy And UV- Visible Spectroscopy’, Journal of advanced pharmaceutical technology & research, pp. 2129–2132.
12. Mathew, M.G. et al. (2020) ‘Evaluation of adhesion of Streptococcus mutans, plaque accumulation on zirconia and stainless steel crowns, and surrounding gingival inflammation in primary molars: randomized controlled trial’, Clinical oral investigations, 24(9), pp. 3275–3280.
13. ‘Nanoparticles: Properties, applications and toxicities’ (2019) Arabian Journal of Chemistry, 12(7), pp. 908–931.
14. Raj Preeth, D. et al. (2021) ‘Bioactive Zinc(II) complex incorporated PCL/gelatin electrospun nanofiber enhanced bone tissue regeneration’, European journal of pharmaceutical sciences: official journal of the European Federation for Pharmaceutical Sciences, 160, p. 105768.
15. Ramamurthy, J. and Professor and Head, Department of Periodontics, Saveetha dental
college and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, 162, PH Road, Chennai 600077, India. (2021) ‘Green Synthesis of Copper oxide Nanoparticles Using Aqueous Extract of Ocimum sanctum and Analysis of Antimicrobial, Anti-inflammatory and Cytotoxic activity of Ocimum sanctum Copper Oxide Nanoparticles - An in vitro study’, International journal of dentistry and oral science, pp. 2848–2852.
16. Rehman, A., John, P. and Bhatti, A. (2021) ‘Biogenic Selenium Nanoparticles: Potential Solution to Oxidative Stress Mediated Inflammation in Rheumatoid Arthritis and Associated Complications’, Nanomaterials, 11(8), p. 2005.
17. Saivarshine, S. et al. (2021) ‘Anti-inflammatory and Antioxidant Activity of White Pepper Oleoresin Mediated Selenium Nanoparticles’, Journal of Pharmaceutical Research International, pp. 312–320. Available at: https://doi.org/10.9734/jpri/2021/v33i62b35585.
18. Sakthi, S. and Department of Public Health Dentistry, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University (2021) ‘Thymus vulgaris mediated selenium nanoparticles, characterization and its antimicrobial activity - an in vitro study’, International journal of dentistry and oral science, pp. 3516–3521.
19. ‘Selenium nanoparticles: A potent chemotherapeutic agent and an elucidation of its mechanism’ (2018) Colloids and surfaces. B, Biointerfaces, 170, pp. 280–292.
20. Sundaram, R., Nandhakumar, E. and Haseena Banu, H. (2019) ‘Hesperidin, a citrus flavonoid ameliorates hyperglycemia by regulating key enzymes of carbohydrate metabolism in streptozotocin-induced diabetic rats’, Toxicology mechanisms and methods, 29(9), pp. 644–653.
21. Sushanthi and Department of Oral and Maxillofacial Surgery, Saveetha Dental College, SIMATS, Saveetha University, Chennai (2021) ‘Vernonia amygdalina mediated copper nanoparticles and its characterization and antimicrobial activity - an in vitro study’, International journal of dentistry and oral science, pp. 3330–3334.
22. Website (no date a). Available at: Singh, J., Dutta, T., Kim, KH. et al. ‘Green’ synthesis of metals and their oxide nanoparticles: applications for environmental remediation. J Nanobiotechnol 16, 84 (2018). https://doi.org/10.1186/s12951-018-0408-4.
23. Website (no date b). Available at: Gopika M. Nair, T. Sajini, Beena Mathew, Advanced green approaches for metal and metal oxide nanoparticles synthesis and their environmental applications, Talanta Open, Volume 5, 2022,100080, ISSN 2666-8319, https://doi.org/10.1016/j.talo.2021.100080.
24. Website (no date c). Available at: Johnson, J., Shanmugam, R., & Lakshmi, T. (2022). A review on plant-mediated selenium nanoparticles and its applications. Journal of population therapeutics and clinical pharmacology = Journal de la therapeutique des populations et de la pharmacologie clinique, 28(2), e29–e40. https://doi.org/10.47750/jptcp.2022.870.
25. Website (no date d). Available at: Cremonini, E., Zonaro, E., Donini, M., Lampis, S., Boaretti, M., Dusi, S., Melotti, P., Lleo, M. M., & Vallini, G. (2016). Biogenic selenium nanoparticles: characterization, antimicrobial activity and effects on human dendritic cells and fibroblasts. Microbial biotechnology, 9(6), 758–771. https://doi.org/10.1111/1751-7915.12374.
26. Website (no date e). Available at: https://ui.adsabs.harvard.edu/link_gateway/2019MaTec..34..490T/doi:10.1080/10667857.2019.1583408.
27. Website (no date f). Available at: Prashant Y. Mali, Shital S. Panchal, Euphorbia tirucalli L.: Review on morphology, medicinal uses, phytochemistry and pharmacological activities, Asian Pacific Journal of Tropical Biomedicine, Volume 7, Issue 7, 2017, Pages 603-613, ISSN 2221-1691, https://doi.org/10.1016/j.apjtb.2017.06.002.
28. Website (no date g). Available at: Benjamaa Rania, Moujanni Abdelkarim, Kaushik Neha, Choi Eun Ha, Essamadi Abdel Khalid, Kaushik Nagendra Kumar TITLE=Euphorbia species latex: A comprehensive review on phytochemistry and biological activities JOURNAL=Frontiers in Plant Science VOLUME=13 YEAR=2022 URL=https://www.frontiersin.org/articles/10.3389/fpls.2022.1008881 DOI=10.3389/fpls.2022.1008881.
29. Website (no date h). Available at: Munro, B., Vuong, Q. V., Chalmers, A. C., Goldsmith, C. D., Bowyer, M. C., & Scarlett, C. J. (2015). Phytochemical, Antioxidant and Anti-Cancer Properties of Euphorbia tirucalli Methanolic and Aqueous Extracts. Antioxidants (Basel, Switzerland), 4(4), 647–661. https://doi.org/10.3390/antiox4040647.
30. Website (no date i). Available at: Anu Rana, Krishna Yadav, Sheeja Jagadevan, A comprehensive review on green synthesis of nature-inspired metal nanoparticles: Mechanism, application and toxicity, Journal of Cleaner Production, Volume 272, 2020, 122880, ISSN 0959-6526, https://doi.org/10.1016/j.jclepro.2020.122880. (https://www.sciencedirect.com/science/article/pii/S0959652620329255).
31. Website (no date j). Available at: Sharma, G.; Sharma, A.R.; Bhavesh, R.; Park, J.; Ganbold, B.; Nam, J.-S.; Lee, S.-S. Biomolecule-Mediated Synthesis of Selenium Nanoparticles using Dried Vitis vinifera (Raisin) Extract. Molecules 2014, 19, 2761-2770. https://doi.org/10.3390/molecules19032761.
32. Website (no date k). Available at: El-Batal AI, Zaid OA, Noaman E, Effat SI. In vivo and in vitro antitumor activity of modified citrus pectin in combination with selenium nanoparticles against Ehrlich carcinoma cells. [Last accessed on 2020 Dec];Int Pharm Sci Health Care. 2012 6:23–47. Available from: http://www.rspublication.com/ijphc/index.html . [Google Scholar] [Ref list].
33. Website (no date l). Available at: Menon, S., Ks, S. D., R, S., S, R., & S, V. K. (2018). Selenium nanoparticles: A potent chemotherapeutic agent and an elucidation of its mechanism. Colloids and surfaces. B, Biointerfaces, 170, 280–292. https://doi.org/10.1016/j.colsurfb.2018.06.006.
34. Zambonino, M.C. et al. (2021) ‘Green Synthesis of Selenium and Tellurium Nanoparticles: Current Trends, Biological Properties and Biomedical Applications’, International journal of molecular sciences, 22(3). Available at: https://doi.org/10.3390/ijms22030989.
35. Menon, S., Ks, S. D., R, S., S, R., & S, V. K. (2018). Selenium nanoparticles: A potent chemotherapeutic agent and an elucidation of its mechanism. Colloids and surfaces. B, Biointerfaces, 170, 280–292. https://doi.org/10.1016/j.colsurfb.2018.06.006
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Jun 26, 2023
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Georgia Benitha, Rajesh Kumar, Pratibha Ramani, & Karthikeyan Ramalingam. (2023). Anti-inflammatory activity of Euphorbia Tirucalli Mediated Selenium nanoparticles: An in vitro study. Journal of Population Therapeutics and Clinical Pharmacology, 30(16), 275–281. https://doi.org/10.47750/jptcp.2023.30.16.037
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