DEVELOPMENT, CHARACTERIZATION, STABILITY AND TOXICITY EVALUATION OF TOPICAL ETHOSOMAL GEL LOADED WITH BERBERIS VULGARIS L. FRUIT EXTRACT AS A COSMECEUTICAL PRODUCT”
Main Article Content
Keywords
Cosmetic, Berberis vulgaris L., Antioxidant, Nano Ethosomes, Stability testing, Non-invasive in vivo study
Abstract
Berberis vulgaris L. being copious with phenols, flavonoids, alkaloids, and vitamin C along with its value-added organic properties is thought to be a good contender of the cosmetic industry. Study was thus aimed to develop a Berberis vulgaris L. (BV) extract based optimized nano-ethosomal gel and its investigation for skin rejuvenation, acne and hydration effects aided by the increased solubility and trapping of various medicinal agents and plant extract by nanostructured vesicles.
Tyrosinase enzyme inhibition test, phenolic and flavonoid content, and in-vitro SPF (sun protection effect) evaluation of the BV extract were all studied. Zeta size, zeta potential, encapsulation efficiency (%EE), and SEM (scanning electron microscopy) were used to analyse formulations of 09 ethosomal formulations based on BV extract. After being included into the gel system, the skin effects of the improved ethosomal formulation were investigated using minimally invasive in vivo methods. A research on the acute oral toxicity of Berberis vulgaris L. was also conducted to examine any potential harmful effects.
The optimized nano ethosomal formulation (F9) was spherical in shape, 116.5nm in size, −28.5 surface charge, and showed EE 97.38%. The BV loaded ethosomal topical gel was stable physicochemically throughout the study and rheological analysis revealed non-Newtonian behavior. Ex vivo permeation of ethosomal gel exhibited 69.88% release. Statistically ANOVA indicated that in vivo parameter skin effects were significant (P˂.05).
A stable BV loaded nano ethosomal gel system is an inspiring approach for phytoextracts than conventional formulations as a cosmeceutical application that has substantial effects on skin.
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14. Cichorek, Mirosława, Wachulska, Małgorzata, Stasiewicz, Aneta, & Tymińska, Agata. (2013). Skin melanocytes: biology and development. Advances in Dermatology and Allergology/Postȩpy Dermatologii I Alergologii, 30(1), 30.
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16. D’Mello, Stacey AN, Finlay, Graeme J, Baguley, Bruce C, & Askarian-Amiri, Marjan E. (2016). Signaling pathways in melanogenesis. International journal of molecular sciences, 17(7), 1144.
17. Dave, Vivek, Kumar, Dhirendra, Lewis, Shaila, & Paliwal, Sarvesh. (2010). Ethosome for enhanced transdermal drug delivery of aceclofenac. International journal of drug delivery, 2(1).
18. Di Mambro, Valéria M, & Fonseca, Maria JV. (2005). Assays of physical stability and antioxidant activity of a topical formulation added with different plant extracts. Journal of pharmaceutical and biomedical analysis, 37(2), 287-295.
19. Eghbali-Feriz, Samira, Taleghani, Akram, Al-Najjar, Hadi, Emami, Seyed Ahmad, Rahimi, Homa, Asili, Javad, . . . Tayarani-Najaran, Zahra. (2018). Anti-melanogenesis and anti-tyrosinase properties of Pistacia atlantica subsp. mutica extracts on B16F10 murine melanoma cells. Research in pharmaceutical sciences, 13(6), 533.
20. Elsayed, Mustafa MA, Abdallah, Ossama Y, Naggar, Viviane F, & Khalafallah, Nawal M. (2007). Lipid vesicles for skin delivery of drugs: reviewing three decades of research. International journal of pharmaceutics, 332(1-2), 1-16.
21. Espinosa-Leal, Claudia A, & Garcia-Lara, Silverio. (2019). Current methods for the discovery of new active ingredients from natural products for cosmeceutical applications. Planta médica, 85(07), 535-551.
22. Fouladi, Rohollah F. (2012). Aqueous extract of dried fruit of Berberis vulgaris L. in acne vulgaris, a clinical trial. Journal of dietary supplements, 9(4), 253-261.
23. Fu, Rao, Zhang, Yuting, Guo, Yiran, & Chen, Fang. (2014). Antioxidant and tyrosinase inhibition activities of the ethanol-insoluble fraction of water extract of Sapium sebiferum (L.) Roxb. leaves. South African Journal of Botany, 93, 98-104.
24. Gajardo, Sandra, Aguilar, Marilú, Stowhas, Tamara, Salas, Felipe, Lopez, José, Quispe, Cristina, . . . Benites, Julio. (2016). Determination of sun protection factor and antioxidant properties of six Chilean Altiplano plants. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, 15(5), 352-363.
25. Gaspar, LR, & Campos, PMBG Maia. (2003). Rheological behavior and the SPF of sunscreens. International journal of pharmaceutics, 250(1), 35-44.
26. Ghribia, Lotfi, Ghouilaa, Hatem, Omrib, Amel, Besbesb, Malek, & Janneta, Hichem Ben. (2014). Antioxidant and anti–acetylcholinesterase activities of extracts and secondary metabolites from Acacia cyanophylla. Asian Pacific journal of tropical biomedicine, 4, S417-S423.
27. Gilbert, Laura, Picard, Céline, Savary, Géraldine, & Grisel, Michel. (2013). Rheological and textural characterization of cosmetic emulsions containing natural and synthetic polymers: relationships between both data. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 421, 150-163.
28. Hadaruga, Daniel I, Hadaruga, Nicoleta G, Bandur, Geza N, Rivis, Adrian, Costescu, Corina, Ordodi, Valentin L, & Ardelean, Aurel. (2010). Berberis vulgaris extract/β cyclodextrin nanoparticles synthesis and characterization. Rev Chim (Bucharest), 61, 669-675.
29. Hameed, Abdul, Akhtar, Naveed, Khan, Haji Muhammad Shoaib, & Asrar, Muhammad. (2019). Skin sebum and skin elasticity: Major influencing factors for facial pores. Journal of cosmetic dermatology, 18(6), 1968-1974.
30. Iizhar, Syed Ahmed, Syed, Ismail Ahmed, Satar, Rukhsana, & Ansari, Shakeel Ahmed. (2016). In vitro assessment of pharmaceutical potential of ethosomes entrapped with terbinafine hydrochloride. Journal of advanced research, 7(3), 453-461.
31. Ijaz, Musarrat, & Akhtar, Naveed. (2020). Fatty acids based α‐Tocopherol loaded nanostructured lipid carrier gel: In vitro and in vivo evaluation for moisturizing and anti‐aging effects. Journal of cosmetic dermatology, 19(11), 3067-3076.
32. Ijaz, Shakeel, Khan, Haji Muhammad Shoaib, Anwar, Zeeshan, Talbot, Brian, & Walsh, John J. (2019). HPLC profiling of Mimosa pudica polyphenols and their non-invasive biophysical investigations for anti-dermatoheliotic and skin reinstating potential. Biomedicine & Pharmacotherapy, 109, 865-875.
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Feb 1, 2024
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Nayla Javed, Haji Muhammad Shoaib Khan, Naheed Akhtar, Umair Khurshid, Umer Shaukat, Marwah Rehman, Hafiz Arfat Idrees, Muhammad Younus, Muhammad Saleem Qureshi, Zafar Iqbal, Wajeeha Almas, Muhammad Naeem, Rihana Dilshad, Rizwana Dilshad, Muhammad Wajahat, Khadija Karim, & Muhammad Shahzad Khan. (2024). DEVELOPMENT, CHARACTERIZATION, STABILITY AND TOXICITY EVALUATION OF TOPICAL ETHOSOMAL GEL LOADED WITH BERBERIS VULGARIS L. FRUIT EXTRACT AS A COSMECEUTICAL PRODUCT”. Journal of Population Therapeutics and Clinical Pharmacology, 31(1), 1887–1910. https://doi.org/10.53555/jptcp.v31i1.4271
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Author Biographies
Nayla Javed
Department of Pharmaceutics, The Islamia University of Bahawalpur, Bahawalpur 63100, Punjab - Pakistan
Haji Muhammad Shoaib Khan
Department of Pharmaceutics, The Islamia University of Bahawalpur, Bahawalpur 63100, Punjab - Pakistan
Naheed Akhtar
Department of Pharmaceutics, The Islamia University of Bahawalpur, Bahawalpur 63100, Punjab - Pakistan
Umair Khurshid
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur, 63100, Punjab - Pakistan
Umer Shaukat
Rashid Latif College of Pharmacy, Rashid Latif Medical Complex, Lahore, Punjab - Pakistan
Marwah Rehman
Abbas Institute of Medical Sciences, GC University Faisalabad, Layyah Campus, Layyah 31200, Punjab - Pakistan
Hafiz Arfat Idrees
Faculty of Pharmacy, The University of Lahore, Punjab - Pakistan
Muhammad Younus
Department of Pharmacognosy, Faculty of Pharmacy, The Islamia University of Bahawalpur - Pakistan
Muhammad Saleem Qureshi
Akbar Institute of Medical Sciences, Bahawalpur, Punjab - Pakistan
Zafar Iqbal
Bahawalpur College of Pharmacy, BMC Complex, Bahawalpur - Pakistan
Wajeeha Almas
IBADAT International University, Islamabad - Pakistan
Muhammad Naeem
Department of Pharmacy, Shah Abdul Latif University, Khairpur, Sindh - Pakistan
Rihana Dilshad
Sheikh Zayed Medical College, Rahim Yar Khan, Punjab - Pakistan
Rizwana Dilshad
Swedish College of Pharmacy and Allied Health Sciences, Rahim Yar Khan – Pakistan
Muhammad Wajahat
Department of Pharmaceutics, Faculty of Pharmacy, Bahauddin Zakariya University, Multan 60800, Punjab - Pakistan
Khadija Karim
Department of Pharmaceutics, Faculty of Pharmacy, Bahauddin Zakariya University, Multan 60800, Punjab - Pakistan
Muhammad Shahzad Khan
Abbas Institute of Medical Sciences, GC University Faisalabad, Layyah Campus, Layyah 31200, Punjab - Pakistan