FORMULATION AND CHARACTERIZATION OF ALKANNA TINCTORIA LOADED EMULGEL AND ITS IN VITRO AND IN VIVO EVALUATION AS A COSMECEUTICAL PRODUCT
Main Article Content
Keywords
Alkannatinctoria, DPPH, Elastometer, Total phenolic contents, total flavonoids contents, skin pores
Abstract
Background: Herbs and plants with enormous levels of phenolic and flavonoid constituents are commonly utilized as antioxidant agents to reduce the impact of UV radiation on the skin and play a significant role in skin treatments.
Aims: Alkanna tinctoria extract's potential cosmetic benefits including reduction in melanin, erythema, sebum, anti-aging and sun protection effectswere investigated in this study, which also aimed to create a stable emulgel that contained 4% Alkanna tinctoria extract.
Objective: Alkanna tinctoria extract was examined and quantified for its phenol and flavonoid content in order to ascertain its cosmetic effects on human skin.
Methods: Alkanna tinctoria (AT) loaded o/w emulsion were combined with gel containing carbopol-940 as gelling agent to create the emulgel formulation. Same procedure and formulation was adopted for control emulgel without extract. For the duration of a 12-week storage period, an in vitro investigation was conducted to assess how color, liquefaction, pH and hardness changed. 13 healthy human volunteers' cheeks were treated with emulgel in order to assess its cosmetic benefits in comparison to control formulations. Utilizing the Mexameter, Elastometer, Sebumeter and Corneometer, and over the duration of the 12-week trial, facial parameters such as skin pigmentation, moisture content, redness, sebum, and skin elasticity were assessed.
Results: Alkanna tinctoria extract found to have 48.04 ± 0.0033 mg GAE/g of total phenolics, and flavonoids were 8.64 ± 0.0046 mg QE/g. Alkanna tinctoria emulgel significantly efficient at minimizing the sebum, small pores andlarge pores (p ≤ 0.05) while efficient to maximize skin’s elasticity and hydration as compared to control emulgel. Antioxidant activity by DPPH activity was 94.23±0.028.
Conclusion: The flavonoid and phenolic concentration of Alkanna tinctoria extract makes it a potent antioxidant that works to protect skin from photodamage and enhance skin conditions.
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Muhammad Shahzad Khan
Abbas Institute of Medical Sciences, GC. University, Layyah Campus, Layyah.
Mudassar Mazher
Department of Pharmacy, The University of Chenab, Gujrat.
Qazi Adnan
Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
Zafar Iqbal
College of Pharmacy, Bahawalpur medical And Dental College, Bahawalpur, Pakistan.
Umer Shaukat
Rashid Latif College of Pharmacy, Lahore, Pakistan.
Hosh Muhammad Lashari
Department of Pharmacognosy, Faculty of Pharmacy, University of Sindh, Jamshoro Pakistan.
Abdul Quddoos
Department of Pharmacy, The Sahara University, Narowal.
Mehran Khan
College of Pharmacy, Bahawalpur medical And Dental College, Bahawalpur, Pakistan.
Shahzada Khurram
School of Health sciences, University of Management and Technology, Lahore, Pakistan
Umair Khurshid
Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
Naheed Akhtar
Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
Fahad Mehmood Khan
Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
Marwah Rehman
Abbas Institute of Medical Sciences, GC. University, Layyah Campus, Layyah.