Main Article Content

Muhammad Asif Abbas Tahir
Muhammad Naeem


Sperata seenghala, Crude protein, Growth, Feed conversion ratio, live weight gain, red blood cells, haemoglobin, white blood cells, haematology


Present research work was conducted to evaluate the effects of various crude protein (CP) diets on the growth and haematology of catfish, Sperata seenghala. Experimental trial was conducted in Aquaria each having water capacity of 70 L (In triplicate) for 90-days. Fish was fed at 5% of wet body weight, twice a day. Five feeds were formulated comprising of 30%, 35%, 40%, 45% and 50% crude proteins, were designated as T1, T2, T3, T4 and T5. However, T6 was designated as Control group containing fish meal (100%). Sperata seenghala fed with T4 (45% CP) has shown higher average final body weight (47.12±3.38) representing significantly (P<0.01) higher growth than T1 (30% CP), T2 (35% CP) T3 (40% CP) and T5 (50% CP). While lowest average final body weight was observed with fish feed T6 (F.M). Live weight gain (LWG) was observed maximum (31.74±0.98) in feeding group T4 (45% CP) while least live weight gain (LWG) 10.33±1.88 was detected in feeding group T6 (F.M). Similarly, percent live weight Gain (%LWG) was determined maximum 206.37 in T4 (45%CP) representing better growth in T4 (45%CP). Fish efficiently assimilated feed of T4 (45% CP) and displayed least feed conversion ratio (FCR) as 4.10 (p<0.01) while maximum values of FCR were determined in T6 (9.19) and T1 (6.60). Numerous haematological parameters such as WBCs, LYM, MON, GRA, RBCs, HGB, RDW, MCV, MCH, MCHC, PLT and MPV were studied which showed significant effect (p<0.01). Fish with T4 (45%) CP represented maximum haemoglobin Hbg (8.25±0.21) showing highly significant values (p<0.01). Maximum RBCs were observed 2.39±0.09 (106/µL) in T6 while minimum RBC value was observed as 1.69±0.13 (106/µL) in T4. Minimum WBCs (106.64±1.38) were observed with CP T4 (45%) showed highly significant relation (p<0.05), representing unstressed conditions and good health status of fish. The order of WBC’s values observed was T1> T6> T5> T2> T3>T4.

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