Assessment of anti-inflammatory effect of novel Zinc oxide nanoparticles synthesized through a formulation of coffee bean and xylitol

Main Article Content

Khushali K Shah
Subhabrata Maiti
Rajeshkumar Shanmugam
Varun Wadhwani

Keywords

Zinc oxide nanoparticles, coffee bean extract, Xylitol, Anti-inflammatory

Abstract

Aim: The aim of this study is to assess the anti-inflammatory effect of novel zinc oxide nanoparticles synthesized through a formulation of coffee bean and xylitol.
Materials and Method: 10 μL, 20 μL, 30 μL, 40 μL and 50 μL of the nanoparticle solution was taken in 5 test tubes respectively. To each test tube 2 ml of 1% Bovine Serum Albumin (BSA) was added. 390 μL, 380 μL, 370 μL, 360 μL and 350 μL of distilled water was then added to the test tube containing nanoparticles to reach a volume of 400 μL in total in each test tube. Similarly, a standard group with 10 μL, 20 μL, 30 μL, 40 μL and 50 μL of Diclofenac Sodium was taken in 5 test tubes. To each test tube 2 mL of 1% Bovine Serum Albumin (BSA) was added. The test tubes were incubated at room temperature for 10 minutes. Then they were incubated in a water bath at 55°C for around 10 minutes. Absorbance was measured at 660 nm in the UV Spectrophotometer.
% Inhibition was calculated using the following formula: = (Control OD-Sample OD) / (Sample OD x 100)
Result: Anti-inflammatory properties of novel zinc oxide nanoparticles were found to be higher than that of the standard values at 40μL and 50 μL concentration. Percentage of inhibition was found to be the highest at 40 μL (67.89%) as well as 50 μL (72.43%) as compared to that of the standard at 40 μL (88.79%) as well as 50 μL (94.51%)
Conclusion: Coffee bean phytochemicals can boost the bioactivity of ZnO NPs, whereas xylitol can lower inflammation even further. Natural ingredients like coffee beans and xylitol, when combined with ZnO NPs, can provide a safe and effective alternative to traditional anti-inflammatory medications with fewer adverse effects.

Abstract 136 | PDF Downloads 104

References

1. Abdel Razak, F.S., AlJoofy, I.K. and Zaki, N.H. (2022) ‘Evaluation of Immunomodulatory Activity of Lipopolysaccharide Zinc Oxide Nanoparticles (LPS-ZnNPS)’, Archives of Razi Institute, 77(5), pp. 1821–1829.
2. Ahmed, S. et al. (2017) ‘A review on biogenic synthesis of ZnO nanoparticles using plant extracts and microbes: A prospect towards green chemistry’, Journal of photochemistry and photobiology. B, Biology, 166, pp. 272–284.
3. ‘Caffeine-loaded gold nanoparticles conjugated with PLA-PEG-PLA copolymer for in vitro cytotoxicity and anti-inflammatory activity’ (2017) Journal of Industrial and Engineering Chemistry , 51, pp. 113–121.
4. ‘Coffee by-products in topical formulations: A review’ (2021) Trends in Food Science & Technology, 111, pp. 280–291.
5. Ganesh, B. and Senior Lecturer, White Lab- Material Research Centre, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences [SIMATS], Saveetha University, Chennai- 77, India. (2021) ‘Effects of black tea and coffee on the colour stability of glass ionomer cement - an in vitro study’, International journal of dentistry and oral science, pp. 4642–4647.
6. Gang, F. (2020) Nanoparticles in Analytical and Medical Devices. Elsevier.
7. ‘Green synthesis of copper nanoparticles using green coffee bean and their applications for efficient reduction of organic dyes’ (2021) Journal of Environmental Chemical Engineering, 9(4), p. 105331.
8. Kamath, K.A., Nasim, I. and Rajeshkumar, S. (2020) ‘Evaluation of the re-mineralization capacity of a gold nanoparticle-based dental varnish: An study’, Journal of conservative dentistry: JCD, 23(4), pp. 390–394.
9. Lakshmi and Dean -International Affairs, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (2021) ‘Biomedical potential of zinc oxide nanoparticles synthesized using plant extracts’, International journal of dentistry and oral science, pp. 4160–4163.
10. LeBel, G. et al. (2020) ‘Antimicrobial Activity, Biocompatibility and Anti-inflammatory Properties of Cetylpyridinium Chloride-based Mouthwash Containing Sodium Fluoride and Xylitol: An In Vitro Study’, Oral health & preventive dentistry, 18(1), pp. 1069–1076.
11. Makhlouf, A.S.H. and Barhoum, A. (2018) Fundamentals of Nanoparticles: Classifications, Synthesis Methods, Properties and Characterization. William Andrew.
12. Pergolizzi, S. et al. (2020) ‘Evaluation of antioxidant and anti-inflammatory activity of green coffee beans methanolic extract in rat skin’, Natural product research, 34(11), pp. 1535–1541.
13. Raghu, S. and Reader, Department of Conservative Dentistry and Endodontics, Saveetha Dental college and Hospital, Saveetha Institute of Medical and Technical Sciences, Saveetha University (2021) ‘Anticariogenic activity of novel herbal formulations (amla , neem ) mediated silver nanoparticles - an in vitro study’, International journal of dentistry and oral science, pp. 3240–3245.
14. Reddy, A.K. et al. (2018) ‘Comparative evaluation of antimicrobial efficacy of silver, titanium dioxide and zinc oxide nanoparticles against streptococcus mutans’, Pesquisa brasileira em odontopediatria e clinica integrada, 18(1), p. e4150.
15. Shukla, A.K. and Iravani, S. (2018) Green Synthesis, Characterization and Applications of Nanoparticles. Elsevier.
16. Willander, M. (2014) Zinc Oxide Nanostructures: Advances and Applications. CRC Press.
17. Zahoor, S. et al. (2023) ‘Biosynthesis and Anti-inflammatory Activity of Zinc Oxide Nanoparticles Using Leaf Extract of’, BioMed research international, 2023, p. 3280708.
18. Menon VD, Muthusekhar MR. Effectiveness of trypsin-chymotrypsin as an anti inflammatory in maxillofacial trauma-a double blinded randomised clinical trial. Int J Dent Oral Sci 2021;8(7):3196-3200.

Most read articles by the same author(s)

1 2 > >>