Green synthesis of coffee bean and xylitol mediated Zinc oxide nanoparticles and the assessment of its antioxidant activity

Main Article Content

Khushali K Shah
Vaishnavi Rajaraman
Rajeshkumar Shanmugam

Keywords

Zinc oxide nanoparticles, Coffee bean extract, Xylitol, antioxidant, DPPH assay

Abstract

Aim: The aim of this study is to synthesize ZnO NPs from coffee beans and xylitol and evaluate their antioxidant efficacy utilizing the DPHH test.
Materials and method: DPPH assay was used to test the antioxidant activity of biogenic synthesized zinc oxide nanoparticles. Diverse concentrations (10-50 μg/ml) of coffee bean and xylitol extract interceded zinc oxide nanoparticle was mixed with 1 ml of 0.1 mM DPPH in methanol and 450 μl of 50 mM Tris HCl buffer (pH 7.4) and incubated. The percentage of inhibition was determined from the following equation, % inhibition= Absorbance of control- Absorbance of test sample × 100/ Absorbance of control
Result: Inhibition rates for zinc oxide nanoparticles made derived from a formulation of coffee bean and xylitol were 47.9% for 10 μL 60.21,% for 20 μL, 72.3% for 30 μL, 78.3% for 40 μL, and 85.7% for 50 μL. The standard showed 76.56% inhibition at 10 μL, 78.52% at 20 μL, 85.63% at 30 μL, 88.68% at 40 μL, and 93.15% at 50 μL. As a result, the highest level of inhibition, or 50 L, was found to be at higher concentration.
Conclusion: The nanoparticles exhibited substantial antioxidant activity, which could be attributed to the presence of phenolic chemicals in coffee beans. The utilization of green synthesis technologies to create nanoparticles is a viable approach to developing effective and long-lasting antioxidant medications. Further research into the potential applications and efficacy of these nanoparticles in various fields is required.

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References

1. Abdelmigid, H.M. et al. (2021) ‘Comparative analysis of nanosilver particles synthesized by different approaches and their antimicrobial efficacy’, Journal of nanomaterials, 2021, pp. 1–12.
2. ‘Anti-inflammatory activity of silver nanoparticles synthesised using hing oil: An in vitro study’ (2020) International journal of pharmaceutical research, 12(sp1). Available at: https://doi.org/10.31838/ijpr/2020.sp1.422.
3. Bildir, B., Çobanoğlu, D.N. and Kaya, B. (2023) ‘Antioxidant, Antimicrobial, and Neurotoxicity (in Sh-Sy5y Cell Line) Properties of Mg Nanoparticle System (BP@MgNPs) Prepared by Green Synthesis with Bee Pollen’, Chemistry & biodiversity, p. e202201093.
4. Caprioli, G. et al. (2015) ‘The influence of different types of preparation (espresso and brew) on coffee aroma and main bioactive constituents’, International journal of food sciences and nutrition, 66(5), pp. 505–513.
5. Chu, Y.-F. et al. (2009) ‘Roasted coffees high in lipophilic antioxidants and chlorogenic acid lactones are more neuroprotective than green coffees’, Journal of agricultural and food chemistry, 57(20), pp. 9801–9808.
6. Ganapathy, D. and Professor & Head of Department, Department of Prosthodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, India. (2021a) ‘Awareness about medicinal application of gold nanoparticles among dental students’, International journal of dentistry and oral science, pp. 4355–4358.
7. Ganapathy, D. and Professor & Head of Department, Department of Prosthodontics, Saveetha Dental College and Hospitals, Saveetha
Institute of Medical and Technical Sciences, Chennai, India. (2021b) ‘Awareness on medicinal applications of silver nanoparticles among dental students’, International journal of dentistry and oral science, pp. 4376–4379.
8. Ganesh, B. and Senior Lecturer, White Lab- Material Research Centre, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences [SIMATS], Saveetha University, Chennai- 77, India. (2021) ‘Effects of black tea and coffee on the colour stability of glass ionomer cement - an in vitro study’, International journal of dentistry and oral science, pp. 4642–4647.
9. Ghorbanpour, M. et al. (2020) Biogenic Nano-Particles and their Use in Agro-ecosystems. Springer Nature.
10. Kad, A. et al. (2022) ‘Meta-analysis of in-vitro cytotoxicity evaluation studies of zinc oxide nanoparticles: Paving way for safer innovations’, Toxicology in vitro: an international journal published in association with BIBRA, 83, p. 105418.
11. Kar, S. et al. (2022) ‘and study of mixtures cytotoxicity of metal oxide nanoparticles to : a mechanistic approach’, Nanotoxicology, 16(5), pp. 566–579.
12. Koupaei, M.H. et al. (2016) ‘Green synthesis of zinc oxide nanoparticles and their effect on the stability and activity of proteinase K’, RSC advances, 6(48), pp. 42313–42323.
13. Lakshmi and Dean -International Affairs, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (2021) ‘Green synthesis of copper nanoparticles synthesized using black tea and its antibacterial activity against oral pathogens’, International journal of dentistry and oral science, pp. 4156–4159.
14. Nardini, M. et al. (2002) ‘Absorption of phenolic acids in humans after coffee consumption’, Journal of agricultural and food chemistry, 50(20), pp. 5735–5741.
15. Poole, C.P., Jr and Owens, F.J. (2003) Introduction to Nanotechnology. John Wiley & Sons.
16. Rai, M. and Posten, C. (2013) Green Biosynthesis of Nanoparticles: Mechanisms and Applications. CABI.
17. Shukla, A.K. and Iravani, S. (2018) Green Synthesis, Characterization and Applications of Nanoparticles. Elsevier.
18. Shunmugam, R. et al. (2021) ‘Biosynthesis of gold nanoparticles using marine microbe (Vibrio alginolyticus) and its anticancer and antioxidant analysis’, Journal of King Saud University. Science, 33(1), p. 101260.
19. Swathy, S., Roy, A. and Rajeshkumar, S. (2020) ‘Anti-inflammatory activity of Ginger oleoresinmediated Silver nanoparticles’, Journal of advanced pharmaceutical technology & research, 13(10), p. 4591.
20. View of Anti inflammatory and antifungal activity of zinc oxide nanoparticle using red sandalwood extract (no date). Available at: https://doi.org/10.47750/jptcp.2023.30.06.023 (Accessed: 5 May 2023).
21. Yang, H. et al. (2009) ‘Comparative study of cytotoxicity, oxidative stress and genotoxicity induced by four typical nanomaterials: the role of particle size, shape and composition’, Journal of applied toxicology: JAT, 29(1), pp. 69–78.
22. Zhao, Z. et al. (2008) ‘5-caffeoylquinic acid and caffeic acid down-regulate the oxidative stress- and TNF-alpha-induced secretion of interleukin-8 from Caco-2 cells’, Journal of agricultural and food chemistry, 56(10), pp. 3863–3868.

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