Evaluation Of Antibacterial Efficacy Of Carica Papaya Leaf and Seed Extract Against Enterococcus faecalis

Main Article Content

Nishitha Arun
Sindhu Ramesh
Anjali Sankar

Keywords

Carica papaya leaf extract, seed extract, calcium hydroxide, E.faecalis, disk diffusion, zone of inhibition, medical, health

Abstract

Introduction: Carica papaya is a well known medicinal plant. Its medicinal value is especially attributed to the presence of secondary metabolites such as tannins, flavonoids, and saponins. Various parts of papaya are used as a cure for various diseases.
Aim: To determine the antimicrobial activity of papaya leaf and seed extract against endodontic pathogens.
Materials and Method: The antibacterial efficacy of C.papaya leaf and seed extract was determined using the zone of inhibition agar disk diffusion method. The Minimum Inhibitory Concentration was determined using broth dilution method.
Results: E.faecalis showed sensitivity for Papaya leaf, seed extract and calcium hydroxide. The zone of inhibition was significantly greater for Papaya leaf extract when compared to papaya seed extract and calcium hydroxide at 25μg/mL, 50 μg/mL and 100 μg/mL (p<0.05). Both papaya leaf and seed showed the highest zone of inhibition at 100μg/mL. The test results showed potent effects of the Papaya leaf extract with MIC at 25μg/mL and For papaya seed extract MBC at 50μg/mL for E.faecalis.
Conclusion: Carica papaya leaf and seed showed prominent antibacterial efficacy against E.faecalis. The minimum inhibitory concentration of C.papaya leaf extract was 25μg/mL whereas for C.papaya seed extract was 50μg/mL. C.papaya leaf extract showed significant antibacterial efficacy against E.faecalis when compared to C.papaya seed extract and Calcium hydroxide. There was no significant difference in antibacterial efficacy between papaya seed extract and calcium hydroxide against E.faecalis.

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