Molecular study on gene and protein expression analysis of TGF beta1 - An in vitro model to develop drugs towards dentin tissue remodelling
Main Article Content
Keywords
Gene expression,Protein expression,TGF beta1, Dentin tissue remodelling
Abstract
Introduction: TGF-1–3 are one-of-a-kind multifunctional growth factors.TGF-beta1 is active in the reconstruction of the dental pulp tissue, according to recent reports. Furthermore, dental pulp cells can divide into odontoblast-like cells, which will then develop reparative dentine.
Materials and Methods: DEMM were used to culture and preserve 3T3 cells. The cells were incubated with LPS after differentiation. 3t3 Cells were used to extract total RNA. It was necessary to amplify cDNA. Using specific reagents, Rt PCR was used to measure the genes of interest.
Results: TGF-beta1 mRNA has a role in protein expression in lipopolysaccharide (LPS) induced macrophage-like cells.TGF-β signals are largely related to the magnitude and duration and careful regulation of their ligand activation, both temporally and spatially. If appropriately activated, TGF-β signalling plays an important function in tissue remodelling.
Conclusion: TGF-beta1 mRNA has a role in protein expression in lipopolysaccharide (LPS) induced macrophage-like cells.TGF-β signals are largely related to the magnitude and duration and careful regulation of their ligand activation, both temporally and spatially. If appropriately activated, TGF-β signalling plays an important function in tissue remodelling. The evidence suggests that hVEGF has a therapeutic potential in regeneration and other disorders of the pulp.
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12. Zadeh G, Koushan K, Pillo L, Shannon P, Guha A. Role of Ang1 and Its Interaction with VEGF-A in Astrocytomas [Internet]. Vol. 63, Journal of Neuropathology & Experimental Neurology. 2004. p. 978–89. Available from: http://dx.doi.org/10.1093/jnen/63.9.978
13. Nishanthine C, Miglani R, R I, Poorni S, Srinivasan MR, Robaian A, et al. Evaluation of Fluoride Release in Chitosan-Modified Glass Ionomer Cements. Int Dent J. 2022 Dec;72(6):785–91.
14. Pandiyan I, Sri SD, Indiran MA, Rathinavelu PK, Prabakar J, Rajeshkumar S. Antioxidant, anti-inflammatory activity of -mediated selenium nanoparticles: An study. J Conserv Dent. 2022 Jun 13;25(3):241–5.
15. Janani K, Teja KV, Ajitha P. Cytotoxicity of oregano essential oil and calcium hydroxide on L929 fibroblast cell: A molecular level study. J Conserv Dent. 2021 Sep-Oct;24(5):457–63.
16. Ramamurthy S, Thiagarajan K, Varghese S, Kumar R, Karthick BP, Varadarajan S, et al. Assessing the Antioxidant and Anti-inflammatory Activity of Crude Extract. J Contemp Dent Pract. 2022 Apr 1;23(4):437–42.
17. Shukla AK, Iravani S. Green Synthesis, Characterization and Applications of Nanoparticles. Elsevier; 2018. 548 p.
18. Nandakumar M, Nasim I. Effect of intracanal cryotreated sodium hypochlorite on postoperative pain after root canal treatment - A randomized controlled clinical trial. J Conserv Dent. 2020 Nov 5;23(2):131–6.
19. Veerayutthwilai O, Byers MR, Pham TTT, Darveau RP, Dale BA. Differential regulation of immune responses by odontoblasts [Internet]. Vol. 22, Oral Microbiology and Immunology. 2007. p. 5–13. Available from: http://dx.doi.org/10.1111/j.1399-302x.2007.00310.x
20. Arana-Chavez VE, Massa LF. Odontoblasts: the cells forming and maintaining dentine [Internet]. Vol. 36, The International Journal of Biochemistry & Cell Biology. 2004. p. 1367–73. Available from: http://dx.doi.org/10.1016/j.biocel.2004.01.006
21. Smith AJ, Murray PE, Sloan AJ, Matthews JB, Zhao S. Trans-dentinal Stimulation of Tertiary Dentinogenesis [Internet]. Vol. 15, Advances in Dental Research. 2001. p. 51–4. Available from: http://dx.doi.org/10.1177/08959374010150011301
22. Duncan HF, Cooper PR. Clinical Approaches in Endodontic Regeneration: Current and Emerging Therapeutic Perspectives. Springer; 2018. 194 p.
23. Vries IG de, de Vries IG, Wisse E. Ultrastructural localization of dentine phosphoprotein in rat tooth germs by immunogold staining [Internet]. Vol. 91, Histochemistry. 1989. p. 69–75. Available from: http://dx.doi.org/10.1007/bf00501914
24. Sloan AJ, Matthews JB, Smith AJ. TGF-beta receptor expression in human odontoblasts and pulpal cells. Histochem J. 1999 Aug;31(8):565–9.
25. McLachlan JL, Smith AJ, Sloan AJ, Cooper PR. Gene expression analysis in cells of the dentine–pulp complex in healthy and carious teeth [Internet]. Vol. 48, Archives of Oral Biology. 2003. p. 273–83. Available from: http://dx.doi.org/10.1016/s0003-9969(03)00003-7
26. Goldberg M. Phosphorylated Extracellular Matrix Proteins of Bone and Dentin. Bentham Science Publishers; 2012. 464 p.
27. Lucchini M, Romeas A, Couble ML, Bleicher F, Magloire H, Farges JC. TGF beta 1 signaling and stimulation of osteoadherin in human odontoblasts in vitro. Connect Tissue Res. 2002;43(2-3):345–53.
28. He WX, Niu ZY, Zhao SL, Jin WL, Gao J, Smith AJ. TGF-β activated Smad signalling leads to a Smad3-mediated down-regulation of DSPP in an odontoblast cell line [Internet]. Vol. 49, Archives of Oral Biology. 2004. p. 911–8. Available from: http://dx.doi.org/10.1016/j.archoralbio.2004.05.005
29. Wieder R. The Roles of FGF-2 TGF Beta and TGF Beta Receptor 2 in Breast Cancer Dormancy [Internet]. 2003. Available from: http://dx.doi.org/10.21236/ada418963
30. Yan Q, Chen W, Song H, Long X, Zhang Z, Tang X, et al. Tofacitinib Ameliorates Lupus Through Suppression of T Cell Activation Mediated by TGF-Beta Type I Receptor. Front Immunol. 2021 Jul 29;12:675542.
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May 13, 2023
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Anjali Sankar, Sindhu Ramesh, Selvaraj Jayaraman, & Nishitha Arun. (2023). Molecular study on gene and protein expression analysis of TGF beta1 - An in vitro model to develop drugs towards dentin tissue remodelling. Journal of Population Therapeutics and Clinical Pharmacology, 30(10), 413–424. https://doi.org/10.47750/jptcp.2023.30.10.046
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