Anti Inflammatory Activity of Lemon Grass Mint Herbal Formulation and Its Mediated Zinc Oxide Nanoparticles
Main Article Content
Keywords
lemon, grass, consumption, time, cancer, proliferation
Abstract
Introduction: For thousands of years natural products, especially plants and vegetables, have been used to fight against various diseases such as cancer, microbial infections and even neurodegenerative diseases. It has been shown that consumption of plants and vegetables have a direct influence on the proliferation, angiogenesis and metastasis of cancer cells.
Materials and methods: The anti-inflammatory activity in lemongrass mint herbal formulation was evaluated by Bovine serum albumin denaturation and egg albumin denaturation assays using standardized protocols.
Results: The anti-inflammatory of lemongrass and mint extract in zinc oxide nanoparticles was assessed. It was treated with different concentrations (10-50μl) of lemongrass and mint extract for 24h. Lemon grass and mint ZnO NPs treatment significantly decreased the viability compared to control at 24 h time point.
Conclusion: The present data demonstrate that extracts of lemon grass and mint ZnO NPs may inhibit the proliferation of cancer cells and induce apoptosis and could provide protection from oxidative stress diseases due to their high antioxidant molecules content.
References
1. Chaudhari LKD, Jawale BA, Sharma S, Sharma H, Kumar CDM, Kulkarni PA. Antimicrobial activity of commercially available essential oils against Streptococcus mutans. J Contemp Dent Pract. 2012 Jan 1;13(1):71–4.
2. Charles DJ. Antioxidant Properties of Spices, Herbs and Other Sources. Springer Science & Business Media; 2012. 612 p.
3. Altavilla C, Ciliberto E. Inorganic Nanoparticles: Synthesis, Applications, and Perspectives. CRC Press; 2017. 832 p.
4. Alamgir ANM. Therapeutic Use of Medicinal Plants and their Extracts: Volume 2:Phytochemistry and Bioactive Compounds. Springer; 2018. 826 p.
5. Akhtar MS, Swamy MK, Sinniah UR. Natural Bio-active Compounds: Volume 1: Production and Applications. Springer Nature; 2019. 608 p.
6. Bayala B, Bassole IHN, Maqdasy S, Baron S, Simpore J, Lobaccaro JMA. Cymbopogon citratus and Cymbopogon giganteus essential oils have cytotoxic effects on tumor cell cultures. Identification of citral as a new putative anti-proliferative molecule. Biochimie. 2018 Oct;153:162–70.
7. Peter KV. Handbook of Herbs and Spices. Elsevier; 2012. 640 p.
8. Shukla AK, Iravani S. Green Synthesis, Characterization and Applications of Nanoparticles. Elsevier; 2018. 548 p.
9. Ranjbar M, Khakdan F, Mukherjee A. In vitro analysis of green synthesized CuO nanoparticles using Tanacetum parthenium extract for multifunctional applications. Environ Sci Pollut Res Int [Internet]. 2023 Apr 5; Available from: http://dx.doi.org/10.1007/s11356-023-26706-x
10. Asghari-Paskiabi F, Imani M, Rafii-Tabar H, Nojoumi SA, Razzaghi-Abyaneh M. Shortening the sulfur cell cycle by a green approach for bio-production of extracellular metalloid-sulfide nanoparticles. Sci Rep. 2023 Mar 23;13(1):4723.
11. Saquib Q, Faisal M, Al-Khedhairy AA, Alatar AA. Green Synthesis of Nanoparticles: Applications and Prospects. Springer Nature; 2020. 316 p.
12. Ahmad K, Asif HM, Afzal T, Khan MA, Younus M, Khurshid U, et al. Green synthesis and characterization of silver nanoparticles through the ethanolic extract and their enzyme inhibitory activities. Front Chem. 2023 Feb 22;11:1065986.
13. Chota A, George BP, Abrahamse H. Recent Advances in Green Metallic Nanoparticles for Enhanced Drug Delivery in Photodynamic Therapy: A Therapeutic Approach. Int J Mol Sci [Internet]. 2023 Mar 2;24(5). Available from: http://dx.doi.org/10.3390/ijms24054808
14. Issa MAS, Hanan ZK. The biofabrication of ZnO nanoparticles using the green soft technique reduction of Zincum Gluconicum (ZNG) by extracellular mycofiltrate of Pit-L6. J Med Life. 2022 Dec;15(12):1476–87.
15. Ali IAM, Ahmed AB, Al-Ahmed HI. Green synthesis and characterization of silver nanoparticles for reducing the damage to sperm parameters in diabetic compared to metformin. Sci Rep. 2023 Feb 8;13(1):2256.
16. S. Rajeshkumar, S. Venkat Kumar, Arunachalam Ramaiah, Happy Agarwal, T. Lakshmi, Selvaraj Mohana Roopan Biosynthesis of zinc oxide nanoparticles using Mangifera indica leaves and evaluation of their antioxidant and cytotoxic properties in lung cancer (A549) Cells Enzyme and Microbial Technology 117 (2018) 91–95.
17. Rajeshkumar S, Synthesis of Zinc oxide nanoparticles using algal formulation (Padina tetrastromatica and Turbinaria conoides) and their antibacterial activity against fish pathogens Research Journal of Biotechnology, (2018), 13:9, 15-19.
18. H. Agarwal, S. Menon, S.V. Kumar, S. Rajeshkumar, Mechanistic study on antibacterial action of zinc oxide nanoparticles synthesized using green route, Chemico-Biological Interactions 286 (2018): 60-70., doi: 10.1016/j.cbi.2018.03.008.
19. Harini, B., Rajeshkumar, S. & Roy, A. Biomedical Application of Chitosan and Piper Longum-assisted Nano Zinc Oxide–based Dental Varnish. Appl Biochem Biotechnol (2021). https://doi.org/10.1007/s12010-021-03712-8.
20. Shanmugam Rajeshkumar, Jayakodí Santhoshkumar, R. P. Parameswari, S. Saravanan, Sri Renukadevi Balusamy, Kalirajan Arunachalam, "Degradation of Toxic Dye and Antimicrobial and Free Radical Potential of Environmental Benign Zinc Oxide Nanoparticles", Bioinorganic Chemistry and Applications, vol. 2022, Article ID 4513208, 10 pages, 2022. https://doi.org/10.1155/2022/4513208.
21. Abel Saka, Jule Leta Tesfaye, Lamessa Gudata, R. Shanmugam, L. Priyanka Dwarampudi, N. Nagaprasad, Ramaswamy Krishnaraj, S. Rajeshkumar, "Synthesis, Characterization, and Antibacterial Activity of ZnO Nanoparticles from Fresh Leaf Extracts of Apocynaceae, Carissa spinarum L. (Hagamsa)", Journal of Nanomaterials, vol. 2022, Article ID 6230298, 6 pages, 2022. https://doi.org/10.1155/2022/6230298.
22. Happy Agarwal, S Venkat Kumar, and S Rajeshkumar A review on green synthesis of Zinc Oxide nanoparticles – An eco-friendly approach Resource Efficient technologies (2017) 3(4), 406-413. DOI: 10.1016/j.reffit.2017.03.002.
23. Santhoshkumar J, Venkat Kumar S Rajeshkumar S, Synthesis of zinc oxide nanoparticles using plant leaf extract againsturinary tract infection pathogen Resource efficient technologies (2017) http://dx.doi.org/10.1016/j.reffit.2017.05.001.
24. S. Rajeshkumar, Happy Agarwal, S. Venkat Kumar, T. Lakshmi, One-Pot Synthesis of Zinc Oxide Nanoparticles Using Orange Peel Extract and Its Potential Anti-Bacterial Activity International Journal of Pharmaceutical Research, 2018, 10, 3: 574-578
25. J. Sujatha, S. Asokan S. Rajeshkumar Antidermatophytic activity of green synthesised zinc oxide nanoparticles using Cassia alata leaves Journal of Microbiology, Biotechnology and Food sciences (2018) 7 (4) 348-352. doi: 10.15414/jmbfs.2018.7.4.348-352.
26. S. Rajeshkumar, Happy Agarwal, S. Venkat Kumar, T. Lakshmi, Brassica oleracea Mediated Synthesis of Zinc Oxide Nanoparticlesand its Antibacterial Activity against Pathogenic Bacteria Asian Journal of Chemistry; 30,(12) (2018), 2711-2715 https://doi.org/10.14233/ajchem.2018.21562.
27. Agarwal Happy, Menon Soumya, S. Venkat Kumar, S. Rajeshkumar⁎, R. David Sheba,T. Lakshmi, V. Deepak Nallaswamy Phyto-assisted synthesis of zinc oxide nanoparticles using Cassia alata and its antibacterial activity against Escherichia coli Biochemistry and Biophysics Reports 17 (2019) 208–211.
2. Charles DJ. Antioxidant Properties of Spices, Herbs and Other Sources. Springer Science & Business Media; 2012. 612 p.
3. Altavilla C, Ciliberto E. Inorganic Nanoparticles: Synthesis, Applications, and Perspectives. CRC Press; 2017. 832 p.
4. Alamgir ANM. Therapeutic Use of Medicinal Plants and their Extracts: Volume 2:Phytochemistry and Bioactive Compounds. Springer; 2018. 826 p.
5. Akhtar MS, Swamy MK, Sinniah UR. Natural Bio-active Compounds: Volume 1: Production and Applications. Springer Nature; 2019. 608 p.
6. Bayala B, Bassole IHN, Maqdasy S, Baron S, Simpore J, Lobaccaro JMA. Cymbopogon citratus and Cymbopogon giganteus essential oils have cytotoxic effects on tumor cell cultures. Identification of citral as a new putative anti-proliferative molecule. Biochimie. 2018 Oct;153:162–70.
7. Peter KV. Handbook of Herbs and Spices. Elsevier; 2012. 640 p.
8. Shukla AK, Iravani S. Green Synthesis, Characterization and Applications of Nanoparticles. Elsevier; 2018. 548 p.
9. Ranjbar M, Khakdan F, Mukherjee A. In vitro analysis of green synthesized CuO nanoparticles using Tanacetum parthenium extract for multifunctional applications. Environ Sci Pollut Res Int [Internet]. 2023 Apr 5; Available from: http://dx.doi.org/10.1007/s11356-023-26706-x
10. Asghari-Paskiabi F, Imani M, Rafii-Tabar H, Nojoumi SA, Razzaghi-Abyaneh M. Shortening the sulfur cell cycle by a green approach for bio-production of extracellular metalloid-sulfide nanoparticles. Sci Rep. 2023 Mar 23;13(1):4723.
11. Saquib Q, Faisal M, Al-Khedhairy AA, Alatar AA. Green Synthesis of Nanoparticles: Applications and Prospects. Springer Nature; 2020. 316 p.
12. Ahmad K, Asif HM, Afzal T, Khan MA, Younus M, Khurshid U, et al. Green synthesis and characterization of silver nanoparticles through the ethanolic extract and their enzyme inhibitory activities. Front Chem. 2023 Feb 22;11:1065986.
13. Chota A, George BP, Abrahamse H. Recent Advances in Green Metallic Nanoparticles for Enhanced Drug Delivery in Photodynamic Therapy: A Therapeutic Approach. Int J Mol Sci [Internet]. 2023 Mar 2;24(5). Available from: http://dx.doi.org/10.3390/ijms24054808
14. Issa MAS, Hanan ZK. The biofabrication of ZnO nanoparticles using the green soft technique reduction of Zincum Gluconicum (ZNG) by extracellular mycofiltrate of Pit-L6. J Med Life. 2022 Dec;15(12):1476–87.
15. Ali IAM, Ahmed AB, Al-Ahmed HI. Green synthesis and characterization of silver nanoparticles for reducing the damage to sperm parameters in diabetic compared to metformin. Sci Rep. 2023 Feb 8;13(1):2256.
16. S. Rajeshkumar, S. Venkat Kumar, Arunachalam Ramaiah, Happy Agarwal, T. Lakshmi, Selvaraj Mohana Roopan Biosynthesis of zinc oxide nanoparticles using Mangifera indica leaves and evaluation of their antioxidant and cytotoxic properties in lung cancer (A549) Cells Enzyme and Microbial Technology 117 (2018) 91–95.
17. Rajeshkumar S, Synthesis of Zinc oxide nanoparticles using algal formulation (Padina tetrastromatica and Turbinaria conoides) and their antibacterial activity against fish pathogens Research Journal of Biotechnology, (2018), 13:9, 15-19.
18. H. Agarwal, S. Menon, S.V. Kumar, S. Rajeshkumar, Mechanistic study on antibacterial action of zinc oxide nanoparticles synthesized using green route, Chemico-Biological Interactions 286 (2018): 60-70., doi: 10.1016/j.cbi.2018.03.008.
19. Harini, B., Rajeshkumar, S. & Roy, A. Biomedical Application of Chitosan and Piper Longum-assisted Nano Zinc Oxide–based Dental Varnish. Appl Biochem Biotechnol (2021). https://doi.org/10.1007/s12010-021-03712-8.
20. Shanmugam Rajeshkumar, Jayakodí Santhoshkumar, R. P. Parameswari, S. Saravanan, Sri Renukadevi Balusamy, Kalirajan Arunachalam, "Degradation of Toxic Dye and Antimicrobial and Free Radical Potential of Environmental Benign Zinc Oxide Nanoparticles", Bioinorganic Chemistry and Applications, vol. 2022, Article ID 4513208, 10 pages, 2022. https://doi.org/10.1155/2022/4513208.
21. Abel Saka, Jule Leta Tesfaye, Lamessa Gudata, R. Shanmugam, L. Priyanka Dwarampudi, N. Nagaprasad, Ramaswamy Krishnaraj, S. Rajeshkumar, "Synthesis, Characterization, and Antibacterial Activity of ZnO Nanoparticles from Fresh Leaf Extracts of Apocynaceae, Carissa spinarum L. (Hagamsa)", Journal of Nanomaterials, vol. 2022, Article ID 6230298, 6 pages, 2022. https://doi.org/10.1155/2022/6230298.
22. Happy Agarwal, S Venkat Kumar, and S Rajeshkumar A review on green synthesis of Zinc Oxide nanoparticles – An eco-friendly approach Resource Efficient technologies (2017) 3(4), 406-413. DOI: 10.1016/j.reffit.2017.03.002.
23. Santhoshkumar J, Venkat Kumar S Rajeshkumar S, Synthesis of zinc oxide nanoparticles using plant leaf extract againsturinary tract infection pathogen Resource efficient technologies (2017) http://dx.doi.org/10.1016/j.reffit.2017.05.001.
24. S. Rajeshkumar, Happy Agarwal, S. Venkat Kumar, T. Lakshmi, One-Pot Synthesis of Zinc Oxide Nanoparticles Using Orange Peel Extract and Its Potential Anti-Bacterial Activity International Journal of Pharmaceutical Research, 2018, 10, 3: 574-578
25. J. Sujatha, S. Asokan S. Rajeshkumar Antidermatophytic activity of green synthesised zinc oxide nanoparticles using Cassia alata leaves Journal of Microbiology, Biotechnology and Food sciences (2018) 7 (4) 348-352. doi: 10.15414/jmbfs.2018.7.4.348-352.
26. S. Rajeshkumar, Happy Agarwal, S. Venkat Kumar, T. Lakshmi, Brassica oleracea Mediated Synthesis of Zinc Oxide Nanoparticlesand its Antibacterial Activity against Pathogenic Bacteria Asian Journal of Chemistry; 30,(12) (2018), 2711-2715 https://doi.org/10.14233/ajchem.2018.21562.
27. Agarwal Happy, Menon Soumya, S. Venkat Kumar, S. Rajeshkumar⁎, R. David Sheba,T. Lakshmi, V. Deepak Nallaswamy Phyto-assisted synthesis of zinc oxide nanoparticles using Cassia alata and its antibacterial activity against Escherichia coli Biochemistry and Biophysics Reports 17 (2019) 208–211.
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Published
Jun 1, 2023
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How to Cite
Preethi Raj, S Rajeshkumar, & T Lakshmi. (2023). Anti Inflammatory Activity of Lemon Grass Mint Herbal Formulation and Its Mediated Zinc Oxide Nanoparticles. Journal of Population Therapeutics and Clinical Pharmacology, 30(13), 125–129. https://doi.org/10.47750/jptcp.2023.30.13.012
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