A comparison of the efficiency of silver diamine fluoride liquid and gel against organisms causing dental caries - An in vitro study
Main Article Content
Keywords
efficiency, silver, organisms, dental
Abstract
Minimally invasive dentistry is gaining its momentum in recent years as it requires less patient cooperation and less operator efforts. Silver diamine fluoride application is one of the recently introduced approaches in controlling caries progression, especially in early childhood caries (ECC). The chief objective of this study is to correlate the antimicrobial activity of SDF liquid of two different commercial brands and also with SDF gel against the cariogenic microorganisms S. mutans, L. acidophilus and A. comitans. Three similar aliquots of 107 CFU/mL of every organism were incubated after mixing, in a brain-heart infusion broth anaerobically at 37°C f. For S. mutans and L. acidophilus the broths were incubated for 24 hours and A. naeslundii was incubated for 48 hours. The agar plates used for Streptococci, Lactobacilli and Actinomycetes identification were Mitis Salivarius, Rogosa and Actinomyces. The zone of inhibition of each plate were measured for different SDF preparations in millimeters.The anticariogenic activity of all the three SDF preparations were similar and not statistically significant. Overall performance of FAgamin SDF solution was better than the other two preparations.
References
1. Fejerskov O. Changing paradigms in concepts on dental caries: consequences for oral health care. Caries Res. 2004 May;38(3):182–91.
2. Margolis HC, Moreno EC. Composition and cariogenic potential of dental plaque fluid. Crit Rev Oral Biol Med. 1994;5(1):1–25.
3. de Almeida L de FD, Cavalcanti YW, Valença AMG. In vitro antibacterial activity of silver diamine fluoride in different concentrations. Acta Odontol Latinoam. 2011;24(2):127–31.
4. Horst JA, Ellenikiotis H, Milgrom PM. UCSF Protocol for Caries Arrest Using Silver Diamine Fluoride: Rationale, Indications and Consent. Pa Dent J . 2017 Jan;84(1):14, 16–26.
5. Heymann H, Swift EJ, Ritter AV. Sturdevant’s Art and Science of Operative Dentistry. Elsevier/Mosby; 2013. 548 p.
6. Russell AD, Hugo WB. Antimicrobial activity and action of silver. Prog Med Chem. 1994;31:351–70.
7. White RJ. An historical overview of the use of silver in wound management. Br J Community Nurs. 2001 Aug 1;6(Sup1):3–8.
8. Lansdown ABG. Silver in health care: antimicrobial effects and safety in use. Curr Probl Dermatol. 2006;33:17–34.
9. Darouiche RO, Meade R, Mansouri M, Raad II. In vivo efficacy of antimicrobial-coated fabric from prosthetic heart valve sewing rings. J Heart Valve Dis. 1998 Nov;7(6):639–46.
10. Coward JE, Carr HS, Rosenkranz HS. Silver sulfadiazine: effect on the ultrastructure of Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1973 May;3(5):621–4.
11. Richards RM. Antimicrobial action of silver nitrate. Microbios. 1981;31(124):83–91.
12. Fuhrmann GF, Rothstein A. The mechanism of the partial inhibition of fermentation in yeast by nickel ions. Biochim Biophys Acta. 1968 Nov 5;163(3):331–8.
13. Liu BY, Lo ECM, Li CMT. Effect of silver and fluoride ions on enamel demineralization: a quantitative study using micro-computed tomography: Effect of silver and fluoride ions on demineralization. Aust Dent J. 2012 Mar;57(1):65–70.
14. YAMAGA, R. Diamine silver fluoride and its clinical application. J Osaka Univ Dent Sch. 1972;12:1–20.
15. Ahad, Mohammed, and S. Gheena. 2016. “Awareness, Attitude and Knowledge about Evidence Based Dentistry among the Dental Practitioner in Chennai City.” Journal of Advanced Pharmaceutical Technology & Research 9 (11): 1863.
16. Girija, Smiline As, Vijayashree Priyadharsini Jayaseelan, and Paramasivam Arumugam. 2018. “Prevalence of VIM- and GIM-Producing Acinetobacter Baumannii from Patients with Severe Urinary Tract Infection.” Acta Microbiologica et Immunologica Hungarica 65 (4): 539–50.
17. Jangid, Kritika, Aurelian Jovita Alexander, Nadathur Doraiswamy Jayakumar, Sheeja Varghese, and Pratibha Ramani. 2015. “Ankyloglossia with Cleft Lip: A Rare Case Report.” Journal of Indian Society of Periodontology 19 (6): 690–93.
18. Kumar, Anoop, Herald J. Sherlin, Pratibha Ramani, Anuja Natesan, and Priya Premkumar. 2015. “Expression of CD 68, CD 45 and Human Leukocyte Antigen-DR in Central and Peripheral Giant Cell Granuloma, Giant Cell Tumor of Long Bones, and Tuberculous Granuloma: An Immunohistochemical Study.” Indian Journal of Dental Research: Official Publication of Indian Society for Dental Research 26 (3): 295–303.
19. Maheswari, T. N. Uma, Archana Venugopal, Nivedhita Malli Sureshbabu, and Prathiba Ramani. 2018. “Salivary Micro RNA as a Potential Biomarker in Oral Potentially Malignant Disorders: A Systematic Review.” Ci Ji Yi Xue Za Zhi = Tzu-Chi Medical Journal 30 (2): 55–60.
20. Malli Sureshbabu, Nivedhitha, Kathiravan Selvarasu, Jayanth Kumar V, Mahalakshmi Nandakumar, and Deepak Selvam. 2019. “Concentrated Growth Factors as an Ingenious Biomaterial in Regeneration of Bony Defects after Periapical Surgery: A Report of Two Cases.” Case Reports in Dentistry 2019 (January): 7046203.
21. Manohar, Janhvi, and R. Abilasha. 2019. “A Study on the Knowledge of Causes and Prevalance of Pigmentation of Gingiva among Dental Students.” Indian Journal of Public Health Research & Development. https://doi.org/10.5958/0976-5506.2019.01859.x.
22. PradeepKumar, Angambakkam Rajasekaran, Hagay Shemesh, Sundaramurthy Jothilatha, Rangarajan Vijayabharathi, Somasundaram Jayalakshmi, and Anil Kishen. 2016. “Diagnosis of Vertical Root Fractures in RestoredEndodontically Treated Teeth: A Time-Dependent Retrospective Cohort Study.” Journal of Endodontia 42 (8): 1175–80.
23. Sekar, Durairaj, Panagal Mani, M. Biruntha, P. Sivagurunathan, and M. Karthigeyan. 2019. “Dissecting the Functional Role of microRNA 21 in Osteosarcoma.” Cancer Gene Therapy 26 (7-8): 179–82.
24. Subashri, A., and T. N. Uma Maheshwari. 2016. “Knowledge and Attitude of Oral Hygiene Practice among Dental Students.” Journal of Advanced Pharmaceutical Technology & Research 9 (11): 1840
25. Berg JH, Slayton RL. Early Childhood Oral Health. John Wiley & Sons; 2015. 344 p.
26. Kutsch VK, Young DA. New directions in the etiology of dental caries disease. J Calif Dent Assoc. 2011 Oct;39(10):716–21.
27. Christensen GJ. The advantages of minimally invasive dentistry. J Am Dent Assoc. 2005 Nov;136(11):1563–5.
28. Mei ML, Li QL, Chu CH, Yiu CKY, Lo ECM. The inhibitory effects of silver diamine fluoride at different concentrations on matrix metalloproteinases. Dent Mater. 2012 Aug;28(8):903–8.
29. Knight GM, McIntyre JM, Craig GG, Mulyani, Zilm PS, Gully NJ. An in vitro model to measure the effect of a silver fluoride and potassium iodide treatment on the permeability of demineralized dentine to Streptococcus mutans. Aust Dent J. 2005 Dec;50(4):242–5.
30. Chu CH, Mei L, Seneviratne CJ, Lo ECM. Effects of silver diamine fluoride on dentine carious lesions induced by Streptococcus mutans and
Actinomyces naeslundii biofilms. Int J Paediatr Dent. 2012 Jan;22(1):2–10.
31. LI, YJ. Effect of a silver ammonia fluoride solution on the prevention and inhibition of caries. Zhonghua Kou Qiang Ke Za Zhi. 1984;19:97–100.
32. Mei ML, Li Q-L, Chu C-H, Lo EC-M, Samaranayake LP. Antibacterial effects of silver diamine fluoride on multi-species cariogenic biofilm on caries. Ann Clin Microbiol Antimicrob. 2013 Feb 26;12:4.
33. Rosas SGP, Téllez MÁA, Espinoza EV. In vitro efficiency of fluoride-containing compounds on remineralization of carious enamel lesions under cyclic pH conditions. Revista Odontologica Mexicana. 2014;18(2):96–104.
34. Mei M-L, Chu C-H, Low K-H, Che C-M, Lo E-C-M. Caries arresting effect of silver diamine fluoride on dentine carious lesion with S. mutans and L. acidophilus dual-species cariogenic biofilm. Med Oral Patol Oral Cir Bucal. 2013 Nov 1;18(6):e824–31.
35. Ryan KJ, Ray CG. Medical microbiology. McGraw Hill. 2004;4:370.
36. Frank RM, Guillo B, Llory H. Caries dentaires chez le rat gnotobiote inoculé avec Actinomyces viscosus et Actinomyces naeslundii. Arch Oral Biol. 1972 Sep 1;17(9):1249–IN1.
37. Jordan HV, Keyes Ph. Aerobic, Gram-Positive, Filamentous Bacteria As Etiologic Agents Of Experimental Periodontal Disease In Hamsters. Arch Oral Biol. 1964 Jul;9:401–14.
38. Bowden GH. Does assessment of microbial composition of plaque/saliva allow for diagnosis of disease activity of individuals? Community Dent Oral Epidemiol. 1997 Feb;25(1):76–81.
2. Margolis HC, Moreno EC. Composition and cariogenic potential of dental plaque fluid. Crit Rev Oral Biol Med. 1994;5(1):1–25.
3. de Almeida L de FD, Cavalcanti YW, Valença AMG. In vitro antibacterial activity of silver diamine fluoride in different concentrations. Acta Odontol Latinoam. 2011;24(2):127–31.
4. Horst JA, Ellenikiotis H, Milgrom PM. UCSF Protocol for Caries Arrest Using Silver Diamine Fluoride: Rationale, Indications and Consent. Pa Dent J . 2017 Jan;84(1):14, 16–26.
5. Heymann H, Swift EJ, Ritter AV. Sturdevant’s Art and Science of Operative Dentistry. Elsevier/Mosby; 2013. 548 p.
6. Russell AD, Hugo WB. Antimicrobial activity and action of silver. Prog Med Chem. 1994;31:351–70.
7. White RJ. An historical overview of the use of silver in wound management. Br J Community Nurs. 2001 Aug 1;6(Sup1):3–8.
8. Lansdown ABG. Silver in health care: antimicrobial effects and safety in use. Curr Probl Dermatol. 2006;33:17–34.
9. Darouiche RO, Meade R, Mansouri M, Raad II. In vivo efficacy of antimicrobial-coated fabric from prosthetic heart valve sewing rings. J Heart Valve Dis. 1998 Nov;7(6):639–46.
10. Coward JE, Carr HS, Rosenkranz HS. Silver sulfadiazine: effect on the ultrastructure of Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1973 May;3(5):621–4.
11. Richards RM. Antimicrobial action of silver nitrate. Microbios. 1981;31(124):83–91.
12. Fuhrmann GF, Rothstein A. The mechanism of the partial inhibition of fermentation in yeast by nickel ions. Biochim Biophys Acta. 1968 Nov 5;163(3):331–8.
13. Liu BY, Lo ECM, Li CMT. Effect of silver and fluoride ions on enamel demineralization: a quantitative study using micro-computed tomography: Effect of silver and fluoride ions on demineralization. Aust Dent J. 2012 Mar;57(1):65–70.
14. YAMAGA, R. Diamine silver fluoride and its clinical application. J Osaka Univ Dent Sch. 1972;12:1–20.
15. Ahad, Mohammed, and S. Gheena. 2016. “Awareness, Attitude and Knowledge about Evidence Based Dentistry among the Dental Practitioner in Chennai City.” Journal of Advanced Pharmaceutical Technology & Research 9 (11): 1863.
16. Girija, Smiline As, Vijayashree Priyadharsini Jayaseelan, and Paramasivam Arumugam. 2018. “Prevalence of VIM- and GIM-Producing Acinetobacter Baumannii from Patients with Severe Urinary Tract Infection.” Acta Microbiologica et Immunologica Hungarica 65 (4): 539–50.
17. Jangid, Kritika, Aurelian Jovita Alexander, Nadathur Doraiswamy Jayakumar, Sheeja Varghese, and Pratibha Ramani. 2015. “Ankyloglossia with Cleft Lip: A Rare Case Report.” Journal of Indian Society of Periodontology 19 (6): 690–93.
18. Kumar, Anoop, Herald J. Sherlin, Pratibha Ramani, Anuja Natesan, and Priya Premkumar. 2015. “Expression of CD 68, CD 45 and Human Leukocyte Antigen-DR in Central and Peripheral Giant Cell Granuloma, Giant Cell Tumor of Long Bones, and Tuberculous Granuloma: An Immunohistochemical Study.” Indian Journal of Dental Research: Official Publication of Indian Society for Dental Research 26 (3): 295–303.
19. Maheswari, T. N. Uma, Archana Venugopal, Nivedhita Malli Sureshbabu, and Prathiba Ramani. 2018. “Salivary Micro RNA as a Potential Biomarker in Oral Potentially Malignant Disorders: A Systematic Review.” Ci Ji Yi Xue Za Zhi = Tzu-Chi Medical Journal 30 (2): 55–60.
20. Malli Sureshbabu, Nivedhitha, Kathiravan Selvarasu, Jayanth Kumar V, Mahalakshmi Nandakumar, and Deepak Selvam. 2019. “Concentrated Growth Factors as an Ingenious Biomaterial in Regeneration of Bony Defects after Periapical Surgery: A Report of Two Cases.” Case Reports in Dentistry 2019 (January): 7046203.
21. Manohar, Janhvi, and R. Abilasha. 2019. “A Study on the Knowledge of Causes and Prevalance of Pigmentation of Gingiva among Dental Students.” Indian Journal of Public Health Research & Development. https://doi.org/10.5958/0976-5506.2019.01859.x.
22. PradeepKumar, Angambakkam Rajasekaran, Hagay Shemesh, Sundaramurthy Jothilatha, Rangarajan Vijayabharathi, Somasundaram Jayalakshmi, and Anil Kishen. 2016. “Diagnosis of Vertical Root Fractures in RestoredEndodontically Treated Teeth: A Time-Dependent Retrospective Cohort Study.” Journal of Endodontia 42 (8): 1175–80.
23. Sekar, Durairaj, Panagal Mani, M. Biruntha, P. Sivagurunathan, and M. Karthigeyan. 2019. “Dissecting the Functional Role of microRNA 21 in Osteosarcoma.” Cancer Gene Therapy 26 (7-8): 179–82.
24. Subashri, A., and T. N. Uma Maheshwari. 2016. “Knowledge and Attitude of Oral Hygiene Practice among Dental Students.” Journal of Advanced Pharmaceutical Technology & Research 9 (11): 1840
25. Berg JH, Slayton RL. Early Childhood Oral Health. John Wiley & Sons; 2015. 344 p.
26. Kutsch VK, Young DA. New directions in the etiology of dental caries disease. J Calif Dent Assoc. 2011 Oct;39(10):716–21.
27. Christensen GJ. The advantages of minimally invasive dentistry. J Am Dent Assoc. 2005 Nov;136(11):1563–5.
28. Mei ML, Li QL, Chu CH, Yiu CKY, Lo ECM. The inhibitory effects of silver diamine fluoride at different concentrations on matrix metalloproteinases. Dent Mater. 2012 Aug;28(8):903–8.
29. Knight GM, McIntyre JM, Craig GG, Mulyani, Zilm PS, Gully NJ. An in vitro model to measure the effect of a silver fluoride and potassium iodide treatment on the permeability of demineralized dentine to Streptococcus mutans. Aust Dent J. 2005 Dec;50(4):242–5.
30. Chu CH, Mei L, Seneviratne CJ, Lo ECM. Effects of silver diamine fluoride on dentine carious lesions induced by Streptococcus mutans and
Actinomyces naeslundii biofilms. Int J Paediatr Dent. 2012 Jan;22(1):2–10.
31. LI, YJ. Effect of a silver ammonia fluoride solution on the prevention and inhibition of caries. Zhonghua Kou Qiang Ke Za Zhi. 1984;19:97–100.
32. Mei ML, Li Q-L, Chu C-H, Lo EC-M, Samaranayake LP. Antibacterial effects of silver diamine fluoride on multi-species cariogenic biofilm on caries. Ann Clin Microbiol Antimicrob. 2013 Feb 26;12:4.
33. Rosas SGP, Téllez MÁA, Espinoza EV. In vitro efficiency of fluoride-containing compounds on remineralization of carious enamel lesions under cyclic pH conditions. Revista Odontologica Mexicana. 2014;18(2):96–104.
34. Mei M-L, Chu C-H, Low K-H, Che C-M, Lo E-C-M. Caries arresting effect of silver diamine fluoride on dentine carious lesion with S. mutans and L. acidophilus dual-species cariogenic biofilm. Med Oral Patol Oral Cir Bucal. 2013 Nov 1;18(6):e824–31.
35. Ryan KJ, Ray CG. Medical microbiology. McGraw Hill. 2004;4:370.
36. Frank RM, Guillo B, Llory H. Caries dentaires chez le rat gnotobiote inoculé avec Actinomyces viscosus et Actinomyces naeslundii. Arch Oral Biol. 1972 Sep 1;17(9):1249–IN1.
37. Jordan HV, Keyes Ph. Aerobic, Gram-Positive, Filamentous Bacteria As Etiologic Agents Of Experimental Periodontal Disease In Hamsters. Arch Oral Biol. 1964 Jul;9:401–14.
38. Bowden GH. Does assessment of microbial composition of plaque/saliva allow for diagnosis of disease activity of individuals? Community Dent Oral Epidemiol. 1997 Feb;25(1):76–81.
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Apr 11, 2023
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Ivan Obadiah, Ganesh Jeevanandan, & S Rajeshkumar. (2023). A comparison of the efficiency of silver diamine fluoride liquid and gel against organisms causing dental caries - An in vitro study. Journal of Population Therapeutics and Clinical Pharmacology, 30(6), 157–162. https://doi.org/10.47750/jptcp.2023.30.06.021
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