Cytotoxicity And Antimicrobial Activity Of Papain And Bromelain Gel Against Oral Microbes

Main Article Content

S B Divya
S.Delphine Priscilla Antony S
M.S.Nivedhitha
S Rajeshkumar

Keywords

Papain, Bromelain, cytotoxicity, antimicrobial,Tree species, Children

Abstract

Background: Traditionally caries removal has been always done using dental burs. There are other methods of caries removal as well. Chemico mechanical method of caries removal is one of the well known methods. This study is papain gel and bromelain gel which have been self prepared and used as caries removal agent.
Materials and methods: Two separate caries removal gels were prepared using papain and bromelain as their key ingredients. The cytotoxicity of the gels were tested by Brine Shrimp Lethality Assay. Antimicrobial activity was evaluated against S mutans, E faecalis, S aureus and C albicans oral bacteria.
Results: Antimicrobial properties of bromelain gel for Candida albicans, Enterococcus faecalis and Streptococcus mutans was comparable with that of control group antibiotic amoxicillin. Antimicrobial properties of papain gel for all the tested four oral microbes was not very much comparable with that of the control amoxicillin antibiotic group.
Bromelain showed a lesser cytotoxic effect with 80% 0f nauplii being alive after 24 hours compared to papain gel as 70% of the nauplii were alive after 24 hours.
Conclusion: Bromelain gel showed better antimicrobial and lesser cytotoxic effects compared to Papain gel. 

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