SYNERGISTIC EFFECT OF SILVER NANOPARTICLES AND ANTIBIOTICS AGAINST METHICILLIN RESISTANCE STAPHYLOCOCCUS AUREUS

Abstract

Silver nanoparticles are the emerging Therapeutic molecules for life and medical field which have many characteristics in their nature act as drug carrier, have antibacterial, antifungal and antiviral activity.  Green technology used for the synthesis of silver nanoparticles (AgNPs) gained significant importance in recent year within research community due to their simple, nontoxic, less time consumption, cost effective nature and also due to their effectiveness in large scale production. The present research study was focused on biosynthesis of AgNPs from the extract of Azadirachta indica and then different analytical techniques such as UV visible spectroscopy, Scanning Electron Microscopy (SEM), and Fourier Transform Infrared Spectroscopy (FTIR) were used for the characterization of AgNPs. In addition, the capability of AgNPs was also determined to enhance the activity of commercially available antibiotics Oxacillin 1µg, ceftazidime 30µg, ceftriaxone 30µg, cefotaxime 30µg, and meropenem 10µg, against bacterial isolates i.e.  Staphylococcus aureus isolated from urine and wounds. In the current study, it was observed that the UV-visible spectroscopy gave absorption peak at 450 nm which was in the prescribed range and confirmed the synthesis of AgNPs. The SEM micrograph demonstrated the morphology of AgNPs. FTIR analysis gave confirmation about capping of AgNPs. Furthermore, it was suggested that AgNPs synthesized from the extract of Azadirachta indica could be of great importance in the pharmaceutical and medical science for their bio catalytic activities while, the combination of AgNPs and antibiotics have great effect against MDR Bacterial species.