IN VITRO EVALUATION OF SESUVIUM SESUVIOIDES (FENZL) VERDC. (AIZOACEAE) OF CHOLISTAN DESERT FOR ANTI-OXIDANT, ANTIMICROBIAL AND CYTOTOXIC POTENTIAL

Main Article Content

Maria Pervaiz
Sabira Sultana
Naheed Akhtar
Aisha Sethi
Muhammad Khawar Abbas
Rida Siddique
Faheem Hadi
Rimsha Khan
Abdul Wadood Chishti

Keywords

Antimicrobial activity, Cytotoxic Potential, Plant extract, antioxidant activity

Abstract

The aim of current study was to explore the antioxidant, antimicrobial and cytotoxic potential of different extracts (Sesuvium sesuvioides ethanol (SS E), Sesuvium sesuvioides n-hexane (SS nh, Sesuvium sesuvioides distilled water (SS DW).  Antioxidant activity of extracts of selected plant were determined by using 2, 2-diphenyl-1-picrylhydrazyl radical scavenging assay as designated by researchers. Folin-Ciocaletu reagent method was used to determine total phenolic contents (TPC). The total flavonoid content (TFC) of the extracts were determined by aluminum chloride complex forming assay. The agar well diffusion method was used, against gram positive and negative bacterial strains. The hemolytic activity was determined by Spectro-photometric method for different extracts against red blood cells and PBS were be used as negative control and Triton-X-100 as positive control. The Sesuvium sesuvioides ethanol extract showed maximum antioxidant (radical scavenging) effects 66.667% with IC50 value (81.48±1.22) μg/mL which was significant (P<0.01). Maximum effect of TPC (mg GAE/g) was exhibited by n-hexane extract 172.4±1.22 mg GAE/g. Similarly, the n-hexane extract showed maximum TFC activity 21.428±2.02mg QE/g. Among three extracts of Sesuvium sesuvioides, maximum zone of inhibition against E. coli and B. subtilis was shown by distilled water extract [(18±0.333) mm], [(19±0.577) mm] respectively. n-hexane extract showed higher hemolytic activity than other extracts (32.525%). Thus, results of study showed that extract of Sesuvium sesuvioides exhibit antimicrobial, cytotoxic and antioxidant potential.

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References

1. Ahmad, S., Alam, K., Wariss, H., Anjum, S., & Mukhtar, M. (2014). Ethnobotanical studies of plant resources of Cholistan desert, Pakistan. Int J Sci Res, 3(6), 1782-1788.
2. Ali, I., Chaudhry, M. S., & Farooq, U. (2009). Camel rearing in Cholistan Desert of Pakistan. Pakistan Veterinary Journal, 29(2).
3. Biharee, A., Sharma, A., Kumar, A., & Jaitak, V. (2020). Antimicrobial flavonoids as a potential substitute for overcoming antimicrobial resistance. Fitoterapia, 146, 104720.
4. Dewan, S. M. R., Amin, M. N., Adnan, T., Uddin, S. N., Shahid-Ud-Daula, A., Sarwar, G., & Hossain, M. S. (2013). Investigation of analgesic potential and in vitro antioxidant activity of two plants of Asteraceae family growing in Bangladesh. Journal of pharmacy research, 6(6), 599-603.
5. El Shaer, H., & Attia-Ismail, S. (2015). Halophytic and salt tolerant feedstuffs in the Mediterranean basin and Arab region: an overview. Halophytic and salt-tolerant feedstuffs impact on nutrition, physiology and reproduction of livestock. Boca Raton: CRC Press Taylor & Francis Group, 21-36.
6. Fatima, T., Mehnaz, S., Wang, M., Yang, J., Sajid, M. S., Shen, B., & Zhao, J. (2019). Seroprevalence of Toxoplasma gondii in one-humped camels (Camelus dromedarius) of Thal and Cholistan deserts, Punjab, Pakistan. Parasitology research, 118(1), 307-316.
7. Hameed, M., Ashraf, M., Al-Quriany, F., Nawaz, T., Ahmad, M. S. A., Younis, A., & Naz, N. (2011). Medicinal flora of the Cholistan desert: a review. Pak. J. Bot, 43(2), 39-50.
8. Hanasaki, Y., Ogawa, S., & Fukui, S. (1994). The correlation between active oxygens scavenging and antioxidative effects of flavonoids. Free Radical Biology and Medicine, 16(6), 845-850.
9. Joshi, A., Kanthaliya, B., & Arora, J. (2018). Halophytes of Thar Desert: Potential source of nutrition and feedstuff. International Journal of Bioassays, 8, 5674-5683.
10. Rafay, M., Ghaffar, M. U., Abid, M., Malik, Z., & Madnee, M. (2021). Phytochemicals analysis and antimicrobial activities of Echinops echinatus from Cholistan Desert, Pakistan. Agrobiological Records, 5, 21-27.
11. Raza, M., Younas, M., & Schlecht, E. (2014). Prevalence of gastrointestinal helminths in pastoral sheep and goat flocks in the Cholistan desert of Pakistan. JAPS: Journal of Animal & Plant Sciences, 24(1).
12. Sajid-Ur-Rehman, M., Ishtiaq, S., Khan, M. A., Alshamrani, M., Younus, M., Shaheen, G., . . . Javed, F. (2021). Phytochemical profiling, in vitro and in vivo anti-inflammatory, analgesic and antipyretic potential of Sesuvium sesuvioides (Fenzl) Verdc.(Aizoaceae). Inflammo pharmacology, 29(3), 789-800.
13. Shabbir, M., Khan, M. R., & Saeed, N. (2013). Assessment of phytochemicals, antioxidant, anti-lipid peroxidation and anti-hemolytic activity of extract and various fractions of Maytenus royleanus leaves. BMC complementary and alternative medicine, 13(1), 1-13.
14. Xie, Y., Yang, W., Tang, F., Chen, X., & Ren, L. (2015). Antibacterial activities of flavonoids: structure-activity relationship and mechanism. Current medicinal chemistry, 22(1), 132-149.
15. Zubair, M., Anwar, F., & Shahid, S. A. (2012). Effect of extraction solvents on phenolics and antioxidant activity of selected varieties of Pakistani rice (Oryza sativa). International Journal of Agriculture and Biology, 14(6).

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