ISOLATION, CHARACTERIZATION, AND MOLECULAR DOCKING STUDIES OF CHLOROFORM FRACTIONATE OF PSAMMOGETON BITERNATUM EDGEW
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Keywords
Medicinal plants, Psammogeton biternatum Edgew, NMR, HMBC, molecular docking, and α-glucosidase
Abstract
Medicinal plants have been widely used in the treatment of various diseases for human health. The present aim of this study was to investigate and identify the bioactive compounds from crude extracts of Psammogeton biternatum Edgew. It belongs to the family Apiaceae. There are numerous advanced techniques were used like NMR and HMBC. Three new pure compounds i.e., compound 1, compound 2 and compound 3 were isolated from the chloroform fraction of the plant. These compounds were screened for enzyme inhibition which was found more potent than the standard. In addition, an in silico molecular docking analysis was also performed with the target enzyme α-glucosidase which potential target for diabetic patients. Among all the three compounds, compound 3 showed the best docking score of -7.6 kcal/mol with the target enzyme. Moreover, in the future, this study can shed light on the development of plant therapeutics for diabetic patients.
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Nida Munir
Centre of Biotechnology and Microbiology, University of Peshawar
Bashir Ahmad
Centre of Biotechnology and Microbiology, University of Peshawar
Aishma Khattak
Department of Bioinformatics, Shaheed Benazir Bhutto Women University Peshawar
Nida Gul
Deptartmemt of Environmental Sciences University of Swabi.
Muhammad Idrees
Department of Health and biological sciences, Abasyn university Peshawar.
Sudhair Abbas Bangash
Faculty of Life Science, department of pharmacy sarhad University of science and information technology, Peshawar.