PHYTOSYNTHESIS, CHARACTERIZATION AND PHARMACOLOGICAL INVESTIGATION OF GOLD NANOPARTICLES USING LEAVES OF ACACIA MODESTA WALL

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Taj Muhammad
Aamir Aziz
Awais Khalid
Bader S. Alotaibi
Nain Taara Bukhari
Iqbal Nisa
Fawad Khan
Nabila Qayum
Yosra Modafer
Farah Shireen
Muhammad Saqib Khalil

Keywords

Phytosynthesis, A. modesta Wall, Characterization, AuNPs, Antimicrobial, Antileshmanial, Cytotoxic

Abstract

Aqueous leaves extracts of Acacia modesta Wall. was utilized to phyto-synthesize green AuNPs. The leaves of A. modesta were investigated to possess potential phytochemicals such as alkaloids, flavonoids, polyphenols, reducing sugars, tannins, saponins and steroids, which help in the bioconversion of Au+3 to Auo. The synthesized AuNPs were analyzed to be stable nano-crystalline spherical, having a size span of 20 – 100 nm. The λmax peak for AuNPs was observed at 550 nm, which manifests the precise fabrication of AuNPs. The sharp Auo peak, along with other organic elements, i.e., C, N, O, Ca++, Mg++, Na+ and Cl- at variable intensities in EDX analysis, manifest phytochemicals in plant extracts have effectively reduced the ionic Au by acting as efficient biocapping and bioreducing agent. The pharmacological activities of these AuNPs in comparison to aqueous leaves extract manifest that AuNPs possess excellent antifungal activity against F. solani (92%), F. oxysporum (91%), M. fur fur (90%), Penecillium and C. albicans (89%). Moderate to good antibacterial activity was analyzed against S. aureus (62%) and K. pnuemoniae (53%). Less cytotoxic activity was observed against brine shrimp nauplii i.e. 6.67% at higest sample concentration i.e. 1000 µg/mL. Good anti-leshmanial activity was manifested by green AuNPs against L. major, showing IC50 value of 50.2 + 0.01 respectively.

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