ANTIOXIDANT AND ANTIGLYCATION POTENTIAL OF DEVELOPED POLYHERBAL FORMULATIONS AND EXTRACTED PHYTOCHEMICALS

Taj Muhammad; Zahida Parveen; Saira Farman; Beenish Khurshid; Muhammad Assad; Nosheen Faiz; Naeema Begum

doi:10.53555/jptcp.v30i18.2794

Taj Muhammad
Zahida Parveen
Saira Farman
Beenish Khurshid
Muhammad Assad
Nosheen Faiz
Naeema Begum

Keywords

Diabetes Mellitus, antioxidant, AGEs, polyherbal formulations, antiglycation

Abstract

Diabetes mellitus is a chronic metabolic disorder characterized by insulin deficiency or its ineffectiveness though produced by the body. More than 90% of patients affected by diabetes are of type II. Advanced Glycated End products (AGEs) in correlation with oxidative stress are mainly responsible for the long-term complications of diabetes. The present study was designed to evaluate antioxidant and antiglycation properties of three individual plants including Trillidium goanum, Centorium tenuiflorum and Rubinia pseudoacacia as well as polyherbal formulations (n=11). Results of crude extracts revealed highest antioxidant and antiglycation activity of polyherbal formulation S22 (Centorium tenuiflorum+Morchella conica) with IC₅₀ values of 21.63±0.15µg/mL and 10.63±1.72µg/mL respectively. When the data was compared for phytochemicals, it was noticed that phenols and tannins from S28 (Trillidium goanum+Centorium tenuiflorum+Morchella conica+Rubinia pseudoacacia) showed the potent antioxidant activity by showing IC₅₀ values of 7.6±0.36µg/mL and 8.17± 1.69 µg/mL respectively. However, for antiglycation properties phenols and tannins for S28 and S29 were found to be potent. The IC50 values were 7.046±3.19µg/mL (phenols, S28), 7.33±2.2µg/mL (tannins, S28), 5.34±1.86µg/mL (Phenols, S29) and 58.07±1.59µg/mL (tannins, S29). Additionally, quantification data indicated highest concentrations of phenols, flavonoids, and tannins in polyherbal formulation S28 which gives an indication of highest antioxidant and antiglycation potential of formulation S28.

Abstract 270 | pdf Downloads 169

References

1. Bukhari, O. M., Ahmed, A. A., Al Kabany, M. H., & Afifi, I. K. (2021). Journal of Umm Al-Qura University for Medical Sciences. Journal of Umm Al-Qura University for Medical Sciences, 7(1), 1-5.
2. Faruk, E. J. E. J. o. H. (2022). Pancreatic protection elicited by platelet-rich plasma and cinnamon combination in a rat model of type 1 diabetes: is it a new era in islet cell regeneration and insulin signaling genes 45(3), 720-737.
3. Akhtar, S., Nasir, J. A., Abbas, T., & Sarwar, A. (2019). Diabetes in Pakistan: A systematic review and meta-analysis. Pakistan journal of medical sciences, 35(4), 1173.
4. Baron, E. J., Miller, J. M., Weinstein, M. P., Richter, S. S., Gilligan, P. H., Thomson Jr, R. B., . . . Dunne, W. M. J. C. i. d. (2013). A guide to utilization of the microbiology laboratory for diagnosis of infectious diseases: 2013 recommendations by the Infectious Diseases Society of America (IDSA) and the American Society for Microbiology (ASM) a. 57(4), e22-e121.
5. Guariguata, L., Whiting, D. R., Hambleton, I., Beagley, J., Linnenkamp, U., Shaw, J. E. J. D. r., & practice, c. (2014). Global estimates of diabetes prevalence for 2013 and projections for 2035. 103(2), 137-149.
6. Inzucchi, S. E., Bergenstal, R. M., Buse, J. B., Diamant, M., Ferrannini, E., Nauck, M., . . . Matthews, D. R. J. D. c. (2015). Management of hyperglycemia in type 2 diabetes, 2015: a patient-centered approach: update to a position statement of the American Diabetes Association and the European Association for the Study of Diabetes. 38(1), 140-149.
7. Nishikawa, T., Edelstein, D., Du, X. L., Yamagishi, S.-i., Matsumura, T., Kaneda, Y., . . . Hammes, H.-P. J. N. (2000). Normalizing mitochondrial superoxide production blocks three pathways of hyperglycaemic damage. 404(6779), 787-790.
8. Pasupathi, P., Chandrasekar, V., Kumar, U. S. J. D., Research, M. S. C., & Reviews. (2009). Evaluation of oxidative stress, enzymatic and non-enzymatic antioxidants and metabolic thyroid hormone status in patients with diabetes mellitus. 3(3), 160-165.
9. Al-Rawi, N. H. J. O. S. D. (2012). Diabetes, oxidative stress, antioxidants and saliva: a review. 8, 303-310.
10. Halliwell, B., & Gutteridge, J. M. (2015). Free radicals in biology and medicine: Oxford university press, USA.
11. Rangarajan, H., Elumalai, A., & Chidanand, D. V. J. J. o. F. B. (2021). Traditional fruits of South India: Bioactive components and their potential health implications in chronic diseases. 45(3), e13266.
12. Kapali, J. (2021). Biological Studies of Some Selected Medicinal Plants from Kathmandu Valley and Isolation of Chemical Compounds. Department of Chemestry
13. Nathan, D. M., Balkau, B., Bonora, E., Borch-Johnsen, K., Buse, J. B., Colagiuri, S., . . . Holman, R. R. J. C. B. C. B. (2010). International expert committee report on the role of the A1C assay in the diagnosis of diabetes. 10(1), 25-33.
14. Thomson, I. J. I. J. o. P. (2017). Mohd Iqbal Yatoo, Archana Saxena, Arumugam Gopalakrishnan, Mahmoud Alagawany and Kuldeep Dhama. 13(7), 732-745.
15. Sun, J., & Buys, N. J. J. B. J. o. N. (2016). Glucose-and glycaemic factor-lowering effects of probiotics on diabetes: a meta-analysis of randomised placebo-controlled trials. 115(7), 1167-1177.
16. Bordoloi, R., & Dutta, K. N. J. J. P. C. B. S. (2014). A review on herbs used in the treatment of diabetes mellitus. 2, 86-92.
17. Kumar, K., Fateh, V., Verma, B., & Pandey, S. J. I. J. R. D. P. L. S. (2014). Some herbal drugs used for treatment of diabetes. 3(5), 1116-1120.
18. Shah, S. B., Parveen, Z., Bilal, M., Sartaj, L., Bibi, S., Nasir, A., & Mahmood, A. J. P. J. o. P. S. (2018). Assessment of antimicrobial, antioxidant and cytotoxicity properties of Camellia sinensis L. 31(4).
19. Begum, N., Nasir, A., Parveen, Z., Muhammad, T., Ahmed, A., Farman, S., . . . Khurshid, A. J. F. i. p. (2021). Evaluation of the hypoglycemic activity of Morchella conica by targeting protein tyrosine phosphatase 1B. 12, 661803.
20. Akindahunsi, A., Oyetayo, F. J. L.-F. S., & Technology. (2006). Nutrient and antinutrient distribution of edible mushroom, Pleurotus tuber-regium (fries) singer. 39(5), 548-553.
21. Brand-Williams, W., Cuvelier, M.-E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food science and Technology, 28(1), 25-30.
22. Nakagawa, T., Yokozawa, T., Terasawa, K., Shu, S., & Juneja, L. R. (2002). Protective activity of green tea against free radical-and glucose-mediated protein damage. Journal of Agricultural and Food Chemistry, 50(8), 2418-2422.
23. Piero, M., Nzaro, G., Njagi, J. J. A. j. o. b., & sciences, p. (2015). Diabetes mellitus-a devastating metabolic disorder. 5(40), 1.
24. Nguyen, T., Locke, S., & Reddy, K. J. I. R. o. F. A. (2015). Ownership concentration and corporate performance from a dynamic perspective: Does national governance quality matter? , 41, 148-161.
25. Pandit, S., Kanjilal, S., Awasthi, A., Chaudhary, A., Banerjee, D., Bhatt, B., . . . Jaggi, M. J. J. o. e. (2017). Evaluation of herb-drug interaction of a polyherbal Ayurvedic formulation through high throughput cytochrome P450 enzyme inhibition assay. 197, 165-172.
26. Telapolu, S., Kalachavedu, M., Punnoose, A. M., Bilikere, D. J. B. c., & medicine, a. (2018). MD-1, a poly herbal formulation indicated in diabetes mellitus ameliorates glucose uptake and inhibits adipogenesis–an in vitro study. 18(1), 1-11.
27. Fernando, C. D., Karunaratne, D. T., Gunasinghe, S. D., Cooray, M., Kanchana, P., Udawatte, C., . . . medicine, a. (2016). Inhibitory action on the production of advanced glycation end products (AGEs) and suppression of free radicals in vitro by a Sri Lankan polyherbal formulation Nawarathne Kalka. 16(1), 1-8.
28. Gupta, M., Karmakar, N., Sasmal, S. J. I. J. o. P., & Research, P. (2017). In vitro antioxidant activity of aqueous and alcoholic extracts of polyherbal formulation consisting of Ficus glomerata Roxb. and Symplocos racemosa Roxb. stem bark assessed in free radical scavenging assays. 9(2).
29. Firuzi, O., Miri, R., Tavakkoli, M., & Saso, L. J. C. m. c. (2011). Antioxidant therapy: current status and future prospects. 18(25), 3871-3888.
30. Aslam, M. S., Ahmad, M. S., Mamat, A. S., Ahmad, M. Z., & Salam, F. (2016). Antioxidant and wound healing activity of polyherbal fractions of Clinacanthus nutans and Elephantopus scaber. Evidence-based Complementary and Alternative Medicine.
31. Raphael, B. J., Hruban, R. H., Aguirre, A. J., Moffitt, R. A., Yeh, J. J., Stewart, C., . . . Getz, G. J. C. c. (2017). Integrated genomic characterization of pancreatic ductal adenocarcinoma. 32(2), 185-203. e113.
32. Ouyang, J., Xu, Q., Chu, C.-W., Yang, Y., Li, G., & Shinar, J. J. P. (2004). On the mechanism of conductivity enhancement in poly (3, 4-ethylenedioxythiophene): poly (styrene sulfonate) film through solvent treatment. 45(25), 8443-8450.

pdf

Published

Oct 19, 2023

DOI https://doi.org/10.53555/jptcp.v30i18.2794

How to Cite

Taj Muhammad, Zahida Parveen, Saira Farman, Beenish Khurshid, Muhammad Assad, Nosheen Faiz, & Naeema Begum. (2023). ANTIOXIDANT AND ANTIGLYCATION POTENTIAL OF DEVELOPED POLYHERBAL FORMULATIONS AND EXTRACTED PHYTOCHEMICALS. Journal of Population Therapeutics and Clinical Pharmacology, 30(18), 748–761. https://doi.org/10.53555/jptcp.v30i18.2794

Issue

Vol. 30 No. 18 (2023): JPTCP

Section

Articles

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

All articles published in JPTCP are licensed under Copyright Creative Commons Attribution-NonCommercial 4.0 International License.

Author Biographies