Quantitative Characterisation of Inorganic and Organic Content of Sharpey’s Fibres

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Ramya Ramadoss
Sandhya Sundar
Suganya Panneer Selvam
Pratibha Ramani


Sharpey’s fibres, cementum, alveolar bone, periodontal regeneration, mineral composition


Introduction: The periodontal ligament's main responsibilities include supporting the teeth, producing the force necessary for tooth eruption, and providing sensory data about the location of the teeth as well as forces that will help the reflex jaw movement that occurs during chewing motions. Sharpey’s fibres are a matrix of connective tissue consisting of bundles of collagen fibre connecting PDL to bone and cementum. Periodontal regeneration is very crucial as it involves reviving the hard and soft tissue interface in the presence of infection and inflammation. Understanding the organic and inorganic composition of these fibres present at the hard and soft tissue interface is crucial in deriving effective regeneration strategies.
Materials and methods: The tooth sections on the glass coverslip were seen using a field emission scanning electron microscope. Sections were dried with nitrogen gas after being dehydrated with 70 percentage ethyl alcohol. The pieces were sputter coated with platinum to induce conductivity at a critical point drying. The images were recorded.
Results: The results revealed that the interface was very different at the cemental surface and the alveolar bone interface. So this will help in terms of formulating newer agents because we have very clearly formed out the percentages of the minerals present in it.
Conclusion: These varied chemical gradients throw inputs about the composition of sharpey’s fibres which exhibited presence of some organic and inorganic components.

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