Quantitative Characterisation of Inorganic and Organic Content of Sharpey’s Fibres

Main Article Content

Jeshurun.J
Ramya Ramadoss
Sandhya Sundar
Suganya Panneer Selvam
Pratibha Ramani

Keywords

Sharpey’s fibres, cementum, alveolar bone, periodontal regeneration, mineral composition

Abstract

Introduction: The periodontal ligament's main responsibilities include supporting the teeth, producing the force necessary for tooth eruption, and providing sensory data about the location of the teeth as well as forces that will help the reflex jaw movement that occurs during chewing motions. Sharpey’s fibres are a matrix of connective tissue consisting of bundles of collagen fibre connecting PDL to bone and cementum. Periodontal regeneration is very crucial as it involves reviving the hard and soft tissue interface in the presence of infection and inflammation. Understanding the organic and inorganic composition of these fibres present at the hard and soft tissue interface is crucial in deriving effective regeneration strategies.
Materials and methods: The tooth sections on the glass coverslip were seen using a field emission scanning electron microscope. Sections were dried with nitrogen gas after being dehydrated with 70 percentage ethyl alcohol. The pieces were sputter coated with platinum to induce conductivity at a critical point drying. The images were recorded.
Results: The results revealed that the interface was very different at the cemental surface and the alveolar bone interface. So this will help in terms of formulating newer agents because we have very clearly formed out the percentages of the minerals present in it.
Conclusion: These varied chemical gradients throw inputs about the composition of sharpey’s fibres which exhibited presence of some organic and inorganic components.

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References

1. Beube, F.E. (1949) ‘Factors in the repair of alveolar bone and cementum’, Oral Surgery, Oral Medicine, Oral Pathology, pp. 379–403. Available at: https://doi.org/10.1016/0030-4220(49)90367-9.
2. Chantarawaratit, P. et al. (2014) ‘Acemannan sponges stimulate alveolar bone, cementum and periodontal ligament regeneration in a canine class II furcation defect model’, Journal of Periodontal Research, pp. 164–178. Available at: https://doi.org/10.1111/jre.12090.
3. chellappa, L.R., Pradeep, K.R. And Prabakar, J. (2020) ‘Association Between Non Nutritive Oral Behaviour With Severity Of Malocclusion In Patients Attending A Dental Hospital In Chennai’, PalArch’s Journal of Archaeology of Egypt / Egyptology, 17(7), pp. 3233–3243.
4. Colard, T. et al. (2016) ‘New Insights on the Composition and the Structure of the Acellular Extrinsic Fiber Cementum by Raman Analysis’, PloS one, 11(12), p. e0167316.
5. Foster, B.L. et al. (2015) ‘Mineralization defects in cementum and craniofacial bone from loss of bone sialoprotein’, Bone, 78, pp. 150–164.
6. Ganapathy, D. et al. (2022) ‘Recent Breakthrough of Bismuth-Based Nanostructured Materials for Multimodal Theranostic Applications’, Journal of nanomaterials, 2022. Available at: https://doi.org/10.1155/2022/4944320.
7. Grandfield, K. et al. (2015) ‘Strain-guided mineralization in the bone-PDL-cementum complex of a rat periodontium’, Bone reports, 3. Available at: https://doi.org/10.1016/j.bonr.2015.04.002.
8. Hirashima, S. et al. (2020) ‘Three-dimensional ultrastructural imaging and quantitative analysis of the periodontal ligament’, Anatomical science international, 95(1). Available at: https://doi.org/10.1007/s12565-019-00502-5.
9. Hirashima, S. et al. (2022) ‘3D mesoscopic architecture of a heterogeneous cellular network in the cementum–periodontal ligament–alveolar bone complex’, Microscopy, pp. 22–33. Available at: https://doi.org/10.1093/jmicro/dfab051.
10. Kato, K. et al. (1990) ‘Distribution of Fluoride across Cementum, Dentine and Alveolar Bone in Rats’, Caries Research, pp. 117–120. Available at: https://doi.org/10.1159/000261251.
11. Li, C., Fan, M. and Tang, Z. (1997) ‘[Detection of types I, III and IV collagen in human cementum, periodontal ligament and alveolar bone]’, Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology, 32(2). Available at: https://pubmed.ncbi.nlm.nih.gov/10677951/ (Accessed: 2 March 2023).
12. Maria, R. et al. (2019) ‘An unusual disordered alveolar bone material in the upper furcation region of minipig mandibles: A 3D hierarchical structural study’, Journal of structural biology, 206(1). Available at: https://doi.org/10.1016/j.jsb.2019.02.010.
13. Mestriner, G. et al. (2022) ‘Histological analysis of ankylothecodonty in Silesauridae (Archosauria: Dinosauriformes) and its implications for the evolution of dinosaur tooth attachment’, Anatomical record , 305(2). Available at: https://doi.org/10.1002/ar.24679.
14. Momose, T. et al. (2016) ‘Collagen Hydrogel Scaffold and Fibroblast Growth Factor-2 Accelerate Periodontal Healing of Class II Furcation Defects in Dog’, The open dentistry journal, 10, pp. 347–359.
15. Rameshkumar, D. et al. (2021) ‘A Correlation and path analysis studies on yield and yield components in brinjal (Solanum melongena L.)’, Electronic Journal of Plant Breeding, 12(1), pp. 249–252.
16. Ramesh Kumar, K. and Anbazhagan, V. (2018) ‘Analysis and assessment of heavy metals in soils around the industrial areas in Mettur, Tamilnadu, India’, Environmental monitoring and assessment, 190(9), p. 519.
17. Ripamonti, U., Herbst, N.N. and Ramoshebi, L.N. (2005) ‘Bone morphogenetic proteins in craniofacial and periodontal tissue engineering: experimental studies in the non-human primate Papio ursinus’, Cytokine & growth factor reviews, 16(3). Available at: https://doi.org/10.1016/j.cytogfr.2005.02.006.
18. Shah, M.P. (2020) Removal of Emerging Contaminants Through Microbial Processes. Springer Nature.
19. Smith, K.C.A. and Oatley, C.W. (2004) ‘2.2B The Scanning Electron Microscope and its Fields of Application’, Advances in Imaging and Electron Physics, pp. 111–125. Available at: https://doi.org/10.1016/s1076-5670(04)33006-5.
20. Sunar, S., Sumathi Felicita, A. and Prasanna Arvind T.R (2022) ‘Assessment Of Micro Esthetics In Patients Reporting For Orthodontic Treatment’, Journal of Pharmaceutical Negative Results, pp. 2687–2694.
21. Sykes, A.H. (2001) Sharpey’s Fibres: The Life of William Sharpey, the Father of Modern Physiology in England.
22. Vishnu Prasad, S. et al. (2018) ‘Report on oral health status and treatment needs of 5-15 years old children with sensory deficits in Chennai, India’, Special care in dentistry: official publication of the American Association of Hospital Dentists, the Academy of Dentistry for the Handicapped, and the American Society for Geriatric Dentistry, 38(1), pp. 58–59.

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