RAPD BASED DETECTION OF STAPHYLOCOCCUS AUREUS ISOLATED FROM POULTRY MEAT AND WORKERS IN THE LOCAL MARKET OF PESHAWAR
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Keywords
S. aureus, Antibiotics, PCR, Poultry Mea, Zoonotic
Abstract
Staphylococcus aureus, a significant pathogen in humans and animals, was analyzed for genetic similarities and distances among isolates from poultry meat and workers. Fifty-one samples were collected from various poultry parts (chest, liver, gizzard, and cloaca) and workers at VRI Peshawar. Worker samples were collected via nasal swabs. Out of the 51 samples, 31 (61%) tested positive for S. aureus, with the highest isolation frequency from the gizzard (73%). Antibiotic susceptibility testing revealed 100% resistance to oxacillin and roxithromycin, followed by 93.5% resistance to tetracycline. Linezolid resistance was observed in 48.4% of the isolates. Genetic analysis using RAPD-PCR with primers OLP6, OLP11, and OLP13 resulted in the amplification of 130 alleles. The dendrogram based on these alleles grouped the isolates into three clusters, consisting of ten populations from chickens (C-1 to C-6) and workers (W1 to W4). Primer OLP-13 amplified the most alleles (55), while OLP-06 amplified the fewest (32). The study found varying genetic distances among and between the isolates, with maximum genetic diversity observed between certain chicken and worker isolates. The genetic relatedness of isolates from chicken meat and humans indicates potential zoonotic transmission. This research demonstrates that RAPD-PCR is an effective method for determining genetic distances and similarities among S. aureus isolates from poultry meat and workers, underscoring the importance of monitoring such pathogens to prevent cross-species transmission.
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Shumaila Jamshed
Department of Health and Biological Sciences, Abasyn University Peshawar, KPK, Pakistan
Shaista Jabeen
Khyber Medical University, Institute of Health Sciences, Islamabad
Huma Mir
Department of Biosciences, COMSATS University, Islamabad
Abdul Hannan Yousufi
Department of Medical Laboratory Technology, NCS Institute of Sciences Capital, Campus Islamabad
Hammad Ahmad
Department of Pharmacy, Bashir Institute of Health Science, Islamabad
Musfira Waqar
College of Veterinary Sciences, The University of Agriculture, Peshawar
Ihsan Ullah
Department of Animal Nutrition, The University of Agriculture, Peshawar
Muhammad Akbar
Deparment of Microbiology, Hazara University, Mansehra
Wajeeha Wajid
Department of Biology, University of Haripur, KPK, Pakistan
Mudasser1
Departments of Pharmacy, Abasyn University Peshawar, KPK, Pakistan