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Mubina Tariq
Barkat Ullah
Mansoor Ahmad
Mian Majid Shah
Abid Ali
Wajeeha Wajid
Saqib Ali
Javeria Sajjad
Muhammad Atif Ur Rehman
Tooba Wajid


Skimmia lareoula, Caspase-3, PARP-1, NF-kB, neurotoxicity


The study explored the efficacy of Skimmia lareoula leaf extracts in ameliorating ethanol-induced neurotoxicity in postnatal day 7 rat pups. Utilizing various fractions and nanoparticles derived from the leaves, including oil, crude extract, chloroform, methyl acetate, silver nitrate, and copper sulphate, researchers administered ethanol intraperitoneally at a dose of 5 g/kg. Following a four-hour period, the animals were euthanized, and their brain tissues were subjected to western blotting analysis. Significantly, all four fractions and both nanoparticles exhibited pronounced reductions in neurotoxicity, as indicated by lowered levels of neuroinflammatory markers like NF-kB, along with decreased expression of neurodegenerative markers such as Caspase-3 and PARP-1. These results underscore the promising therapeutic potential of Skimmia lareoula leaf fractions and nanoparticles in mitigating ethanol-induced neurotoxicity. The findings suggest a multifaceted neuroprotective role for Skimmia lareoula, offering insights into its potential application as a therapeutic agent against ethanol-induced neuronal damage. By targeting pivotal pathways implicated in neuroinflammation and neurodegeneration, these extracts and nanoparticles provide a promising avenue for the development of innovative interventions aimed at mitigating ethanol-induced neurotoxicity. Further exploration of the underlying mechanisms governing the neuroprotective effects of Skimmia lareoula constituents could potentially lead to the development of effective therapeutic strategies for ethanol-induced neurotoxicity and associated disorders.

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