POTENTIAL EFFECT OF SKIMMIA LAREOULA LEAVES EXTRACTED DIFFERENT FRACTIONS AND NANOPARTICLES RESCUES YOUNG RATS AGAINST ETHANOL INDUCED NEUROTOXICITY
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Keywords
Skimmia lareoula, Caspase-3, PARP-1, NF-kB, neurotoxicity
Abstract
The study explored the efficacy of Skimmia lareoula leaf extracts in ameliorating ethanol-induced neurotoxicity in postnatal day 7 rat pups. Utilizing various fractions and nanoparticles derived from the leaves, including oil, crude extract, chloroform, methyl acetate, silver nitrate, and copper sulphate, researchers administered ethanol intraperitoneally at a dose of 5 g/kg. Following a four-hour period, the animals were euthanized, and their brain tissues were subjected to western blotting analysis. Significantly, all four fractions and both nanoparticles exhibited pronounced reductions in neurotoxicity, as indicated by lowered levels of neuroinflammatory markers like NF-kB, along with decreased expression of neurodegenerative markers such as Caspase-3 and PARP-1. These results underscore the promising therapeutic potential of Skimmia lareoula leaf fractions and nanoparticles in mitigating ethanol-induced neurotoxicity. The findings suggest a multifaceted neuroprotective role for Skimmia lareoula, offering insights into its potential application as a therapeutic agent against ethanol-induced neuronal damage. By targeting pivotal pathways implicated in neuroinflammation and neurodegeneration, these extracts and nanoparticles provide a promising avenue for the development of innovative interventions aimed at mitigating ethanol-induced neurotoxicity. Further exploration of the underlying mechanisms governing the neuroprotective effects of Skimmia lareoula constituents could potentially lead to the development of effective therapeutic strategies for ethanol-induced neurotoxicity and associated disorders.
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Mubina Tariq
Department of Botany, Islamia College University Peshawar, 25120, Pakistan
Barkat Ullah
Neuro Molecular Medicine Research Center, Ring Road Peshawar, Pakistan
Mansoor Ahmad
Department of Botany, Islamia College University Peshawar, 25120, Pakistan
Mian Majid Shah
Department of Botany, Islamia College University Peshawar, 25120, Pakistan
Abid Ali
Department of Botany bacha khan university charsadda, KPK, Pakistan
Wajeeha Wajid
Department of Zoology, PMAS Arid Agriculture University, Rawalpindi, Pakistan
Saqib Ali
Department of Chemistry, Hazara University Mansehra, KPK, Pakistan
Javeria Sajjad
Department of Microbiology, Hazara University Mansehra, KPK, Pakistan
Muhammad Atif Ur Rehman
Department of Botany bacha khan university charsadda, KPK, Pakistan
Tooba Wajid
Departemt of Biosciences, COMSATS University Islamabad, Pakistan