COMPREHENSIVE ANALYSIS OF COL1A1 EXPRESSION, METHYLATION, AND GENETIC ALTERATIONS IN LIVER HEPATOCELLULAR CARCINOMA
Main Article Content
Keywords
LIHC, COL1A1, Diagnosis, Treatment
Abstract
Liver hepatocellular carcinoma (LIHC) is a prevalent and deadly form of liver cancer characterized by significant genetic and epigenetic alterations. This study focuses on the role of COL1A1, a gene implicated in various cancers, by analyzing its expression, promoter methylation, and genetic mutations in LIHC. Using the UALCAN database, we observed a significant upregulation of COL1A1 in LIHC samples compared to normal controls, suggesting its involvement in cancer progression. This finding was corroborated by GEPIA2 analysis, which also showed elevated COL1A1 expression in LIHC. Further analysis using UALCAN revealed that COL1A1 expression was consistently upregulated across different cancer stages, races, ages, and genders of LIHC patients, indicating its broad role in tumor development. Validation with GEPIA2 confirmed these observations at individual cancer stages. Methylation analysis showed that COL1A1 was hypomethylated in LIHC samples relative to normal controls, a factor known to enhance tumor development. Interestingly, stage-specific analysis indicated hypermethylation of COL1A1 in stage 4 LIHC, reflecting its complex regulatory mechanisms. Survival analysis using KM plotter and GEPIA2 indicated that higher COL1A1 expression was associated with lower overall survival (OS) rates in LIHC patients, although the results were not statistically significant. Genetic alteration analysis via cBioPortal identified a low mutation frequency (3%) in COL1A1, suggesting limited impact on LIHC through genetic mutations alone. In conclusion, our comprehensive analysis highlights COL1A1 as a potentially significant player in LIHC progression through its aberrant expression and methylation, although its direct genetic mutations appear to have minimal effect. These findings underscore the need for further research to fully elucidate COL1A1's role and therapeutic potential in LIHC.
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Zainab lanjar
Department of Veterinary Microbiology, SBBUVAS, Pakistan
Rohma Fatima
Shahida Islam Medical College Lodhran, Pakistan
Maryam Saddiqa
Department of Livestock & Dairy Development Narowal, Pakistan
Muhammad Khizer Hayat
Center for Animal Diagnostics, Chughtai Lab, Lahore, Pakistan
Imtiaz Ali Soomro
Department of Surgery, Peoples University of Medical and Health Sciences Nawabshah, Pakistan
Tayyaba Riaz
Faculty of Biosciences, Cholistan University of Veterinary and Animal Sciences (CUVAS), Bahawalpur, Pakistan
Umair Younas
Department of Livestock Management, Faculty of Animal Production and Technology (FAPT), Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan
Qaiser Akram
Department of Pathobiology (Microbiology), University of Veterinary & Animal Sciences, Lahore, Pakistan (Narowal Campus 51600), Pakistan
Muhammad Ahsan Naeem
Department of Basic Sciences (Pharmacology), University of Veterinary and Animal Sciences, Lahore, Pakistan (Narowal Campus 51600)
Muhammad Adnan Aslam
Deputy Director Oric, UVAS, Lahore, Pakistan
Rizwana Sultan
Department of Pathology, Faculty of Veterinary and Animal Sciences, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan.