COMPREHENSIVE ANALYSIS OF CDCA8 IN COLON ADENOCARCINOMA INCLUDING EXPRESSION, METHYLATION, MUTATIONS, AND PROGNOSTIC SIGNIFICANCE

Main Article Content

Muhammad Imran
Imtiaz Ali Soomro
Kiran Nazish
Naheed Bano
Muhammad Abbas
Muhammad Khizer Hayat
Habibullah Janyaro
Usama Ahmed
Hafeez Ullah
Muhammad Salman Hameed

Keywords

Colon adenocarcinoma, Diagnosis, Treatment, Biomarker

Abstract

In the current study, the role of cell division cycle-associated 8 (CDCA8) in colon adenocarcinoma (COAD) was analyzed through comprehensive expression and methylation analysis, genetic mutation inquiry, and prognostic assessment. Utilizing the UALCAN database, CDCA8 expression analysis revealed significant overexpression in carcinogenic cells compared to normal control samples, suggesting its involvement in COAD proliferation. Further examination of CDCA8 expression across various clinical parameters showed significant upregulation in different cancer development stages, racial groups, genders, and age classes within COAD patients, highlighting its critical role in cancer proliferation. Validation using the GEPIA2.0 tool confirmed that CDCA8 was highly expressed in COAD compared to normal controls. Additionally, analysis of CDCA8 expression across different cancer stages revealed dysregulation in all four stages, with the highest expression in stage I and the lowest in stage III. The study also investigated the promoter methylation level of CDCA8, finding a significant association between COAD samples and normal controls. Analysis of promoter methylation across various clinical parameters showed significant variations, with distinct methylation patterns observed across cancer stages, racial groups, genders, and age groups. Overall survival (OS) and disease-free survival (DFS) analyses using the KM plotter tool demonstrated that low CDCA8 expression was associated with shorter OS compared to high CDCA8 expression. In terms of DFS, COAD patients with higher CDCA8 expression experienced better DFS than those with low CDCA8 expression. Further validation of CDCA8 expression against survival data indicated that high CDCA8 expression was associated with better OS and DFS in COAD. Lastly, mutational assessment using the cBioPortal platform showed no significant mutations in COAD samples. Overall, these findings highlight the complex role of CDCA8 in COAD pathogenesis, underscoring its potential as a prognostic biomarker and therapeutic target in COAD management.

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