ISOLATION AND MOLECULAR CHARACTERIZATION OF ETMIC2 GENE OF EIMERIA TENELLA CAUSING COCCIDIOSIS IN CHICKENS
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Keywords
Eimeria, Poultry, Coccidiosis, Polymerase Chain Reaction, Molecular characterization, Phylogeny
Abstract
The purpose of this study was to isolate the gene of Eimeria tenella species for molecular characterization and analysis. In this study, the evolutionary relationship of microneme gene2 of Eimeria tenella was confirmed by isolation, molecular characterization and phylogenetic analysis. The birds reared for this purpose were infected with oocyst of Eimeria tenella. Samples were collected from caecal lesions that appeared at the postmortem of birds. Oocysts were isolated using different egg floatation techniques. Analysis of EtMic gene 2 was performed by polymerase chain reaction (PCR). All the samples collected from the caecal lesion showed positive results for EtMic2 gene. The samples showing the best result for the EtMic2 gene were sent for sequencing at Macrogen ® Laboratory Korea. It was revealed from the phylogenetic analysis that its similarity index was 97.9% to all worldwide related sequences of the EtMic2 gene. It was revealed from the analysis that isolated gene of EtMic 2 will provide better opportunities for the development of DNA vaccine against different strains of Eimeria tenella in Pakistan.
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Muhammad Arif Khan
Department of Parasitology and Microbiology, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture University Rawalpindi, Pakistan.
Muhammad Ali Abdullah Shah
Department of Parasitology and Microbiology, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture University Rawalpindi, Pakistan.
Murtaz-ul-Hasan
Department of Parasitology and Microbiology, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture University Rawalpindi, Pakistan
Aayesha Riaz
Department of Parasitology and Microbiology, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture University Rawalpindi, Pakistan
Muhammad Shoaib
Department of Parasitology and Microbiology, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture University Rawalpindi, Pakistan.
Abdul Asim Farooq
Department of Clinical Sciences, Bahauddin Zakriya University, Multan, Pakistan.
Muhammad Kamran
Department of Parasitology and Microbiology, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture University Rawalpindi, Pakistan
Saif-ur-Rehman
Department of Parasitology and Microbiology, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture University Rawalpindi, Pakistan.
Zahid Manzoor
Department of Parasitology and Microbiology, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture University Rawalpindi, Pakistan.