CHARACTERIZATION AND ANTI-HEPATIC FIBROSIS ACTIVITY OF AgNPs PRODUCED THROUGH GREEN SYNTHESIS METHOD USING CITRULLUS COLOCYNTHIS PLANT EXTRACT

Main Article Content

Muhammad Ayaz
Ikram Ullah
Nazia Imran
Maryam Iqbal
Sapna Quraish
Yusra Rasheed
Huma Rafiq
Ome Kalsoom Afridi
Sulaiman Shams

Keywords

Silver, Nanoparticles, Liver, Mice, Characterization, Hepatic

Abstract

Nanoparticles have intriguing properties that could assist the study of regenerative medicine. Silver Nanoparticles (AgNPs) have gained a lot of interest in a variety of industries, particularly pharmaceutics, agriculture, water purification, filtration of air, the textile industry, and as a catalyst in oxidation reactions. The aim of the current study was characterized the biosynthesized AgNPs and to evaluate their anti-hepatic activities through in-vivo studies. Biosynthesized AgNPs were characterized using various techniques including UV–VIS spectrophotometry, XRD, FESEM and FTIR analysis. The in-vivo anti-hepatic efficacy of AgNPs were assessed through morphological, biochemical and histological studies. For in-vivo studies, 12 Male Balb/C albino-mice were randomly divided into 3 groups (n = 4) including normal group, disease control group (Liver fibrotic model), and treatment group (liver fibrosis treated with AgNPs). Further, biochemistry markers including bilirubin level was assessed for all treatment groups. In CCl4-induced fibrotic mice, a considerable increased in glycogen was observed after treatment with AgNPs. Biochemical investigations revealed that all biochemical parameters for liver function such as bilrubin, was significantly lowered in AgNPs treated animals. Our findings indicate a preliminary multi-target efficacy for AgNPs against liver fibrotic and thus require further detailed investigation.

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