MELILOTUS INDICUS TOXICITY PROFILE, HISTOPATHOLOGY AND SCREENING OF HYPOTENSIVE ACTIVITY THROUGH NIBP METHOD ON RATS
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Keywords
Melilotus indicus, Toxicity, Histopathology, Screening, Hypotensive activity
Abstract
The present investigation was carried out to evaluate the effect of Melilotus indicus (L.) All. in normotensive and fructose induced hypertensive rats. Aqueous-ethanolic extract of Melilotus indicus in 250, 500 and 750 mg/kg doses was studied in normotensive and fructose induced hypertensive rats using the non-invasive technique. The results obtained showed that the extract has significantly (p < 0.001) decreased the blood pressure and heart rate in dose dependent manner. The dose 750 mg/kg of the extract produced the maximum antihypertensive effect and was selected for further experiments. The extract was found to prevent the rise in blood pressure of fructose fed rats as compared to control group in 08 weeks study. The LD50 of the plant extract was more than 2000mg/kg b.w. in rats and acute toxicity study showed that there was no significant alteration in the blood chemistry of the extract treated rats. Furthermore, histopathological study revealed no injury to heart, liver and kidney tissues. It is conceivable, therefore, that the aqueous-ethanolic extract of Melilotus indicus has exerted considerable antihypertensive activity in rats and has duly supported traditional medicinal use of plant in hypertension.
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Huma Naz
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Government College University Faisalabad 38000 – Pakistan.
Mohammad Saleem
University College of Pharmacy, University of The Punjab, Lahore 54590 – Pakistan.
Alamgeer
University College of Pharmacy, University of The Punjab Lahore - Pakistan.
Taha Muhammad
Shalamar Medical and Dental College, Lahore - Pakistan.
Rizwana Dilshad
Swedish College of Pharmacy and Allied Health Sciences, Rahim Yar Khan - Pakistan.