HEPATOPROTECTIVE ACTIVITY OF RHODIOLA ROSEA ON RATS

Main Article Content

Anand R Hiremath
Roshan. S

Keywords

R.rosea. INH+RF, Liver injury and CAT

Abstract

Rhodiola rosea has been used as food and folk medicine to recover several diseases. In the present study were evaluated effect of ethanolic extract of R.rosea on Isoniazid and Rifampicin induced liver injury on rats. Isoniazid and Rifampicin was administered a 100 and 50 mg/kg bw dose to induced hepatotoxicity. R.rosea (400 and 600mg/kg, p.o ) and Silymarin 100mg/kg, p.o. were administered once daily for 28 days. The liver injury was estimated by liver function function parameters ALP,AST,ALT,TP andTB. Oxidative stress markers GSH, MPO,MDA,SOD CAT as well histopathology of liver. Isoniazid and Rifampicin drugs significantly reduction in liver weight,  increases in serum liver  biochemical, decreses in Oxidative stress and changes in histopathology of liver. Treatment with R.rosea in a dose depended manner significantly changing in weight liver, liver function estimation , lipid peroxidation and histopathology of liver compare to control and Silymarin group. The present studies evidence that the ethanolic extract of R.rosea has shown hepatoprotective activity

Abstract 38 | pdf Downloads 14

References

1. Organization WH, Latent TJG, WHO: infection: updated and consolidated guidelines for programmatic management. 2018.
2. Kumar R, Shalimar BV, Khanal S, Sreenivas V, Gupta SD, Panda SK, Acharya SK. Antituberculosis therapy-induced acute liver failure: magnitude, profile, prognosis, and predictors of outcome. Hepatology. 2010;51(5):1665–74.
3. Makhlouf HA, Helmy A, Fawzy E, El-Attar M, Rashed HA. A prospective study of antituberculous drug-induced hepatotoxicity in an area endemic for liver diseases. Hepatol Int. 2008;2(3):353–60.
4. Pal R, Vaiphei K, Sikander A, Singh K, Rana SV. Effect of garlic on isoniazid and rifampicin-induced hepatic injury in rats. World J Gastroenterol: WJG. 2006;12(4):636.
5. Kelly GS Rhodiola rosea: a possible plant adapatogen. Autern Med Rev. 2001: 6: 293–302.
6. Kurmukov AG, Aizikov MI, Rakhimov SS: Pharmacology of the plant polyphenol epigalokhin. FarmakolToksikol .1986;49: 45–48.
7. Furmanowa M, Skopinska-Rozewska E, Rogala E, Hartwich M: Rhodiola rosea in vitro culture-phytochemical analysis and antioxidant action. Acta Soci Bota Pol. 1998;67: 69–73.
8. Maslov LN, Lishmanov YB, Maimessulova LA, Krashov EA: A mechanism of antiarrhythmic effect of Rhodiola rosea. Bull Exper Biol Med .1998;125: 374–376.
9. Bawa AS, Khanum F. Anti-inflammatory activity of Rhodiolaroseae" a second-generation adaptogen.Phytother Res2009;23:1099e102.
10. Chen TS, Liou SY, Chang YL..Antioxidant evaluation of three adaptogen extracts. Am J Chin Med. 2008;36:1209e17.
11. Skopinska-Rozewska E, Malinowski M, Wasiutynski A, Sommer E, Furmanowa M, Mazurkiewicz M, Siwicki AK.The influence of Rhodiolaquadrifida 50% hydro-alcoholic extract and salidroside on tumor-induced angiogenesis in mice. Pol J Vet Sci; 2008.11:97e104.
12. Sodhi CP, Rana SV, Mehta SK, Vaiphei K, Attari S, Mehta S. Study of oxidative-stress in isoniazid-rifampicin induced hepatic injury in young rats. Drug Chem Toxicol 1997;20:255-69.4.
13. Tostmann A, Boeree M, Aarnoutse R, de Lange W, van der Ven A, Dekhuijzen R. Antituberculosis drug-induced hepatotoxicity: concise up-to-date review. J Gastroenterol Hepatol 2008; 23(2): 192-202.
14. Dillarid GJ et al. Effect of lipid peroxidation. J. Applied physics. 1998; 45: 927
15. Rao GM, Rao CV, Pushpangadan P, Shirwaikar A. Hepatoprotective effects of rubiadin, a major constituent of Rubia cordifolia Linn. J Ethnopharmacol. 2006;103(3):484-490.
16. Sahin E, Gümüşlü S. Stress-dependent induction of protein oxidation, lipid peroxidation and anti-oxidants in peripheral tissues of rats: comparison of three stress models (immobilization, cold and immobilization-cold). Clin Exp PharmacolPhysiol 2007; 34(5-6):425-431.
17. Mahmood DN, Mamat SS, Kamisan HF , Yahya F, Kamarolzaman FFM, Nasir N , Mohtarrudin N, Tohid, and Zakaria AZ . Amelioration of Paracetamol-Induced Hepatotoxicity in Rat by the Administration of Methanol Extract of Muntingia calabura L. Leaves. BioMed Research International. 2014 , 1 -10.
18. Saleem HT , El-Maali AN, Hassan HM , Mohamed AN, Mostafa MAN , Kahaar AE , and Tammam SA. Comparative Protective Effects of N-Acetylcysteine, N-Acetyl Methionine, and N-Acetyl Glucosamine against Paracetamol and Phenacetin Therapeutic Doses–Induced Hepatotoxicity in Rats. International Journal of Hepatology. 2018 , 1-8.
19. R. Pal, K. Vaiphei, A. Sikander, K. Singh, S.V. Rana Effect of garlic on isoniazid and rifampicin-induced hepatic injury in rats World J. Gastroenterol., 12 ;2006; p. 636
20. Tostmann A, Boeree MJ, Peters WH, Roelofs HM, Aarnoutse RE, van der Ven AJ, Dekhuijzen PN. Isoniazid and its toxic metabolite hydrazine induce in vitro pyrazinamide toxicity. Int J Antimicrob Agents. 2008;31(6):577–80.
21. Dillarid GJ et al. Effect of lipid peroxidation. J. Applied physics. 1998; 45: 927
22. Yue J, Peng R, Chen J, Liu Y, Dong G. Effects of rifampin on CYP2E1-dependent hepatotoxicity of isoniazid in rats. Pharmacol Res. 2009;59(2):112–9.
23. Shih TY, Pai CY, Yang P, Chang WL, Wang NC, Hu OY. A novel mechanism underlies the hepatotoxicity of pyrazinamide. Antimicrob Agents Chemother. 2013;57(4):1685–90.
24. Ramappa V, Aithal GP. Hepatotoxicity related to anti-tuberculosis drugs: mechanisms and management. J Clin Exp Hepatol. 2013;3(1):37–49.
25. Tasduq SA, Peerzada K, Koul S, Bhat R, Johri RK. Biochemical manifestations of anti-tuberculosis drugs induced hepatotoxicity and the effect of silymarin. Hepatol Res. 2005;31(3):132–5.
26. Shabbir M, Khan MR, Saeed N. Assessment of phytochemicals, antioxidant, anti-lipid peroxidation and anti-hemolytic activity of extract and various fractions of Maytenus royleanus leaves. BMC Complement Altern Med. 2013;13:143