PHYTOCHEMICAL AND PHARMACOLOGICAL SCREENING OF MOMORDICA DIOICA FOR HEPATOPROTECTIVE ACTIVITY IN RATS

Main Article Content

Kowti Rajesh
Roshan. S

Keywords

Isoniazid and Rifampicin, M. dioica and Silymarin

Abstract

The present study was designed to evaluate hepatoprotective activity of Momordica dioica (M. dioica) in Wistar albino rats using Anti Tuberculosis drugs  induced hepatic damaged experimental animals. Isoniazid and Rifampicin-induced was administered a 100 and 50 mg/kg bw dose to induce hepatotoxicity. M. dioica (300 and 500mg/kg, p.o ) and Silymarin 100mg/kg, p.o. were administered once daily for 30 days. The degree of hepatoprotection was measured using morphological parameters of changing in weight of liver, Biochemical parameters in serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), serum alkaline phosphatase (SALP), bilirubin. The histopathological parameters of histological changes in the liver architecture like architecture of hepatic lobules, swelling of liver cell, fatty changes, focal necrosis, inflammatory cell infiltration around portal areas, kupffer cell hyperplasia etc. The result of this study indicated that the M. dioica has got a hepatoprotective action against Isoniazid and Rifampicin induced hepatic damage in experimental animals.

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References

1. World Health Organization/IUATLD Global project on anti-tuberculous drug Resistance Surveillance. Anti-tuberculous Drug Resistance in the World. Third global report. WHO/HTM/TB/2004.343. Geneva: World Health Organization, 2004.
2. García A, Bocanegra-García V, Palma-Nicolas JP, Rivera G. ´ Recent advances in antitubercular natural products. Eur J Med Chem 2012; 49(1): 1-23.
3. Gunther G. Multidrug-resistant and extensively drug-resistant ¨ tuberculosis: a review of current concepts and future challenges. Clin Med (London) 2014; 14(3): 279-285.
4. Tost JR, Vidal R, Cayla J, Diaz-Cabanela D, Jimenez A, Broquetas JM. Severe hepatotoxicity due to anti-tuberculosis drugs in Spain. Int. J. Tuberc. Lung Dis. 2005; 9: 534–40.
5. Joseph JK, Antony VT. Ethnobotanical investigations in the genus Momordica L.in the Southern Western Ghats of India. Genet Resour Crop Evol. 2008;55:713–21.
6. Kirtikar KR, Basu BD. Vol. 2. Dehradun: International Book Distributors; 1999. Indian Medicinal Plants.
7. Jain A, Soni M, Deb L, Jain A, Rout SP, Gupta VB, et al. Antioxidant and hepatoprotective activity of ethanolic and aqueous extracts of Momordica dioica Roxb. leaves. J Ethnopharmacol. 2008;115:61–6.
8. Jain A, Singhai AK. Nephroprotective activity of Momordica dioica Roxb.in cisplatin-induced nephrotoxicity. Nat Prod Res. 2010;24:846–54.
9. Jain A, Singhai AK. Effect of Momordica dioica Roxb on gentamicin model of acute renal failure. Nat Prod Res. 2010;24:1379–89.
10. Anbarasu C, Rajkapoor B, Kalpana J. Protective effect of Pisonia aculeata on rifampicin and isoniazid induced hepatotoxicity in rats. Int J Phytomed 2011; 3(1): 75-83.
11. Shreedhara CS, Pai KS, Vaidya VP. Postcoital antifertility activity of the roots of Momordica dioica Roxb. Indian J Pharm Sci. 2001;63:528–31.
12. Turner P, Granville-Grossman K, Smart J. Effect of adrenergic receptor blockade on the tachycardia of thyrotoxicosis and anxiety state. Lancet. 1965;286(7426):1316–8.
13. R. Pal, K. Vaiphei, A. Sikander, K. Singh, S.V. Rana Effect of garlic on isoniazid and rifampicin-induced hepatic injury in rats World J. Gastroenterol., 12 ;2006; p. 636
14. T. Uehara, M. Hirode, A. Ono, N. Kiyosawa, K. Omura, T. Shimizu, Y. Mizukawa, T. Miyagishima, T. Nagao, T. Urushidani A toxicogenomics approach for early assessment of potential non-genotoxic hepatocarcinogenicity of chemicals in rats Toxicology, 250 (2008), pp. 15-26
15. Sodhi CP, Rana SV, Mehta SK, Vaiphei K, Attari S, Mehta S. Study of oxidative-stress in isoniazid-rifampicin induced hepatic injury in young rats. Drug Chem Toxicol 1997;20:255-69.4.
16. Sherlock S, Dooley J, editors. Drugs and Liver. In: Diseases of the Liver and Biliary System, 11th Edition. Blackwell Science: Oxford, UK; Malden, MA; 2002:335-63