PHYTOCOMPOUND-BASED DRUG DISCOVERY APPROACH TO EXPLORE THE FROSTBITE HEALING POTENTIAL OF ABIETADIENE ISOLATED FROM PINUS ROXBURGHII
Main Article Content
Keywords
Abstract
Exposure to subzero temperatures usually leads to vascular damage causing severe ischemic injury known as frostbite, one of the prominent cold weather injuries that can lead to devastating consequences such as amputation of the extremities. Although rate of amputation due to frostbite has decreased recently due to thrombolytic therapy but this new regimen comes with a price as well which include symptoms such as profuse gastrointestinal bleeding in patients. Thus, there is a need to discover effective treatments with least side effects and more bioavailability rate. In current work, aqueous extract of Pinus roxburghii leaves were checked against dry-ice induced frostbite on plantar surfaces of albino Wistar rats (both genders, 150-200 g) by keeping heparin as control, followed by the computational evaluation of its phytocompounds to prioritize potential antiinflammatory and anti-thrombotic compounds against frostbite. Statistically analyzed results of molecular docking showed that among all secondary metabolites of P.roxburghii, abietadiene was most suitable potential ligand against antiplasmin and antithrombin III, which modulated anticoagulant pathway and proved to be a valuable anti-inflammatory and antithrombotic agent for wound healing. These results suggest the wound healing potential of abietadiene especially in case of frost bite which further needs to be validated experimentally.
References
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28. Jamshidi-Kia F, Lorigooini Z, Amini-Khoei H. Medicinal plants: Past history and future perspective. J. HerbMed Pharmacol. 2018;7(1). Accessed October 26, 2021. http://eprints.skums.ac.ir/6978/
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30. Akram M, Rashid A. Anti-coagulant activity of plants: mini review. J Thromb Thrombolysis. 2017;44(3):406-411.
31. Ramjan A, Hossain M, Runa JF, Md H, Mahmodul I. Evaluation of thrombolytic potential of three medicinal plants available in Bangladesh, as a potent source of thrombolytic compounds. Avicenna J Phytomed. 2014;4(6):430-436.
32. Jakaria Md, Islam M, Shariful I Md, Belal Talu M, Dhar Clint C, Ibrahim M. Thrombolysis Potential of Methanol Extracts from the Five Medicinal Plants Leaf, Available in Bangladesh. Pharmacologia. 2017;8(2):78-82.
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34. Bisht M, Sekar K, Arya D. Diversity, utilization pattern, threat status and conservation of medicinal plants in great Himalayan national park, Himachal pradesh, western Himalaya. APJR. 2019;1.
35. Gautam T, Gautam P, Keservani R, Sharma A. Phytochemical screening and wound healing potential of Cuscuta reflexa. J. Chin. Pharm. Sci.2015;24(5):292-302.
36. Man R, Samant S. Diversity, indigenous uses and conservation status of medicinal plants in Manali wildlife sanctuary, North Western Himalaya. IJTK. 2011;10(3):439-459.
37. Secim-Karakaya P, Saglam-Metiner P, Yesil-Celiktas O. Antimicrobial and wound healing properties of cotton fabrics functionalized with oil-in-water emulsions containing Pinus brutia bark extract and Pycnogenol® for biomedical applications. Cytotechnology. 2021;73(3):423-431.
38. Shankar, Devkota S, Paudel K, et al. Investigation of antioxidant and anti-inflammatory activity of roots of Rumex nepalensis. WJPPS. 2015;4:582-589.
39. Gupta B, Dass B. Composition of herbage in Pinus roxburghii Sargent stands: basal area and importance value index. Casp. J. Environ. Sci. 2007;5(2):93-98.
40. Siddiqui MF, Ahmed M, Wahab M, et al. Phytosociology of Pinus roxburghii Sargent (chir pine) in lesser Himalayan and Hindu Kush range of Pakistan. Pak J Bot. 2009;41(5):2357-2369.
41. Ahmed M, Husain T, Sheikh AH, Hussain SS, Siddiqui MF. Phytosociology and structure of Himalayan forests from different climatic zones of Pakistan. Pak J Bot. 2006;38(2):361.
42. Hassan A, Amjid I. Gas chromatography-mass spectrometric studies of essential oil of Pinus roxburghii stems and their antibacterial and antifungal activities. J. Med. Plant Res. 2009;3(9):670673.
43. Salem MZM, Ali HM, Basalah MO. Essential Oils from Wood, Bark, and Needles of Pinus roxburghiiSarg. from Alexandria, Egypt: Antibacterial and Antioxidant Activities. Bioresources. 2014;9(4):7454-7466.
44. Labib RM, Youssef FS, Ashour ML, Abdel-Daim MM, Ross SA. Chemical Composition of Pinus roxburghii Bark Volatile Oil and Validation of Its Anti-Inflammatory Activity Using Molecular Modelling and Bleomycin-Induced Inflammation in Albino Mice. Molecules. 2017;22(9):1384.
45. Farooq Z, Iqbal Z, Mushtaq S, Muhammad G, Iqbal MZ, Arshad M. Ethnoveterinary practices for the treatment of parasitic diseases in livestock in Cholistan desert (Pakistan). J Ethnopharmacol. 2008;118(2):213-219.
46. Sajid A, Manzoor Q, Iqbal M, Tyagi AK, Sarfraz RA, Sajid A. Pinus roxburghiiessential oil anticancer activity and chemical composition evaluation. EXCLI J. 2018;17:233-245.
47. Sharma A, Sharma L, Goyal R. GC/MS Characterization, in-vitro Antioxidant, Antiinflammatory and Antimicrobial Activity of Essential Oils from Pinus Plant Species from Himachal Pradesh, India. JEOBP.2020;23(3):522-531.
48. Safaeian L, Zolfaghari B, Assarzadeh N, Ghadirkhomi A. Antioxidant and Anti-hyperlipidemic Effects of Bark Extract of Pinus eldarica in Dexamethasone-induced Dyslipidemic Rats. J Adv Med Biomed Res.2019;27(125):49-56.
49. Tripathi A, Misra K. Molecular docking: A structure-based drug designing approach. JSM Chem. 2017;5(2):1042-1047.
50. Ben Sghaier M, Louhichi T, Hakem A, Ammari Y. Chemical investigation of polar extracts from Ruta chalpensis L. growing in Tunisia: Correlation with their antioxidant activities. J. New Sci. 2018;49(4): 2971-2978.
51. Jijith US, Jayakumari S. An Apparatus for the determination of rat paw Edema during In vivo Evaluation of Anti-inflammatory agents. Research J Pharm and Tech. 2020;13(5):1-3.
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Published
Sep 25, 2023
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Hasan Akbar Khan, Asma Ahmed, Khadija Kiran, Samra Hafeez, Rehana Badar, & Sajjad Ahmad. (2023). PHYTOCOMPOUND-BASED DRUG DISCOVERY APPROACH TO EXPLORE THE FROSTBITE HEALING POTENTIAL OF ABIETADIENE ISOLATED FROM PINUS ROXBURGHII. Journal of Population Therapeutics and Clinical Pharmacology, 30(17), 1968–1986. https://doi.org/10.53555/jptcp.v30i17.2900
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Author Biographies
Hasan Akbar Khan
Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Lahore
Asma Ahmed
Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Lahore
Khadija Kiran
Department of Physiology, Gujranwala Medical College, Gujranwala, Punjab
Samra Hafeez
Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Lahore
Rehana Badar
Department of Biological Sciences Superior University Lahore Punjab, Islamic Republic of Pakistan
Sajjad Ahmad
Department of Health and Biological Sciences, Abasyn University, Peshawar