FOURIER-TRANSFORM INFRARED SPECTROSCOPY, ANTIOXIDANT, ANTI-INFLAMMATORY AND PHYTOCHEMICAL SCREENING OF DIFFERENT EXTRACTS OF PUNICA GRANATUM PEELS
Main Article Content
Keywords
Pomegranate, Antioxidants, Anti-inflammatory, FTIR, Drugs, Phytochemical screening
Abstract
Different types of drugs have been used over the centuries to cure various diseases due to their potential biological activities. However, these drugs have severe side effects on the human body over time. Medicinal plants are known for their great ability to fight against different types of diseases. As a result, it is thought that using these medicinal plants for healing represents an enormous potential source of therapeutic support for maintaining excellent health. Punica granatum is an important medicinal plant with many beneficial constituents that act as anti-inflammatory and antioxidant agents. Four different extracts of Punica granatum peels such as aqueous, methanol, ethyl acetate, and n-hexane were prepared and tested to determine phytochemical screening antioxidant activity and anti-inflammatory activity. Antioxidant activity was performed by Iron chelating activity and FeCl3 reducing power activity at different time intervals. Methanol extract showed the lowest IC50 value (0.2) at different time intervals which shows its higher antioxidant, n-hexane extract showed the highest IC50 value (39.4) at 10 mins time intervals as compared to other extracts. The phytochemical composition of the pomegranate extracts was thoroughly examined using FTIR. This analysis verified the presence of a wide array of bioactive compounds in the pomegranate peels, including carbohydrates, alkaloids, tannins, flavonoids, terpenoids, proteins, glycosides, coumarins, and total phenols. In vitro, the anti-inflammatory activity of all four extracts was carried out. Methanol extract has shown the highest %age inhibition value of 348% for its higher concentration of 100µg/ml. These results showed that pomegranate peels proved to be better antioxidant and anti-inflammatory agents and can be used for medicinal purposes.
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
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Muhammad Hamza
Department of Chemistry, Lahore Garrison University (LGU) Sector-C Avenue-4, phase 6 DHA Lahore
Affifa Tajammal
Department of Chemistry, University of Engineering and Technology, Lahore 54890
Tayyab Abas
Department of Chemistry, University of Engineering and Technology, Lahore 54890
Rimsha
Department of Chemistry, Lahore Garrison University (LGU) Sector-C Avenue-4, phase 6 DHA Lahore
Hafiz Muhammad Rayan
Department of Chemistry, Lahore Garrison University (LGU) Sector-C Avenue-4, phase 6 DHA Lahore