ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL POTENTIAL OF EUPHORBIA HELIOSCOPIA L. COLLECTED FROM SIALKOT PAKISTAN

Main Article Content

Hafsa Khalid
Zahid Ali Butt
Fatima Idrees
Fiza Munir
Anfal Arshad
Maria Akhtar
Salma Khalid
Samra Riasat
Asra Imtiaz
Javed Kamar
Sajid Hussain

Keywords

.

Abstract

A study was conducted to evaluate the antimicrobial activity and phytochemical analysis of Euphorbia helioscopia L. Plants were collected and tested against several common species and human pathogenic microorganisms including Escherichia coli, Klebsiella sp. and the pathogenic fungus Aspergillus niger. Different solvents viz., ethanol, methanol, acetone and water were used for plant extract preparation. The extract then goes through a preliminary screening process to determine the presence of important phytochemicals. Phytochemical analysis has shown that Euphorbia helioscopia contains many active compounds, including flavonoids, alkaloids, tannins and saponins. Antifungal and antibacterial activity was evaluated by agar well diffusion method. Maximum zone of inhibition was observed by ethanolic and methanolic extract of E. helioscopia against E. coli, Klebsiella sp and A. niger as compared to other extracts. The extracts prepared in the water showed minimum zone of inhibition.

Abstract 228 | pdf Downloads 93

References

1. Abou-El-Hamd, E. M., Mohamed-Elamir, F. H., and Moustafa, M. F. (2012). Euphorbia helioscopia: chemical constituents and biological activities. International Journal of Phytopharmacology, 3(1), 78-90.
2. Adil, M., Filimban, F. Z., Ambrin, Quddoos, A., Sher, A. A., and Naseer, M. (2024). Phytochemical screening, HPLC analysis, antimicrobial and antioxidant effect of Euphorbia parviflora L. (Euphorbiaceae Juss.). Scientific Reports, 14(1), 5627.
3. Ahmed, S. R., Roy, R., Romi, I. J., Hasan, M., Bhuiyan, M. K. H., and Khan, M. M. H. (2019). Phytochemical screening, antioxidant and antibacterial activity of some medicinal plants grown in Sylhet region. IOSR J. Pharm. Biol. Sci, 14, 26-37.
4. Amtaghri, S., Akdad, M., Slaoui, M., and Eddouks, M. (2022). Traditional uses, pharmacological, and phytochemical studies of Euphorbia: A review. Current Topics in Medicinal Chemistry, 22(19), 1553-1570.
5. Bakht, J., Shafi, M., and Iqbal, A. (2017). In vitro antibacterial and antifungal activities of different solvent extracted samples from the stem of Euphorbia heliscopia. Pakistan Journal of Pharmaceutical Sciences, 30(4), 1371-1377.
6. Beltagy, A. M. (2019). Chemical composition and antibacterial activity of medicinally useful essential oil from the inflorescence of Euphorbia helioscopia L. grown in Egypt. International Journal of Pharmaceutical Sciences and Research, 10(8), 3660-7.
7. Deshpande, R. K., Reddy, V. J. S., Jallepalli, V. R., and Dwivedee, M. (2024). Evaluation of acute oral toxicity of aqueous extract of Euphorbia helioscopia. 6(si2):552. DOI:10.48047/AFJBS.6.Si2.2024.5527-5539
8. Dou, M., Zhu, D., Cui, G., Li, H., Di, L., & Wang, L. (2024). Euphorbia helioscopia L. exhibits promising therapeutic effects on hemangioendothelioma and melanoma through angiogenesis inhibition. Phytomedicine, 129, 1556-66.
9. Doughari, J. H. (2012). Phytochemicals: extraction methods, basic structures and mode of action as potential chemotherapeutic agents (pp. 1-33). Rijeka, Croatia: INTECH Open Access Publisher.
10. Falana, M. B and Nurudeen, Q. O. (2022). Phytochemical screening and in vitro antimicrobial activities of Euphorbia Lateriflora on selected pathogens. Iraqi Journal of Science, 1402-1412.
11. Jaouadi, R., Elimem, M., Sayari, N., Kalboussi, M., Lahfef, C., Hammadi, J. and Rouz, S. (2022). Antioxidant and insecticidal properties of Euphorbia helioscopia L. Aqueous extracts. International Journal of Research in Agronomy. 5(2): 91-94.
12. Kebede, T., Gadisa, E. and Tufa, A. (2021). Antimicrobial activities evaluation and phytochemical screening of some selected medicinal plants: A possible alternative in the treatment of multidrug-resistant microbes. PloS one, 16(3). e0249253.
13. Kuppusamy, P., Yusoff, M. M., Parine, N. R. and Govindan, N. (2015). Evaluation of in-vitro antioxidant and antibacterial properties of Commelina nudiflora L. extracts prepared by different polar solvents. Saudi journal of biological sciences, 22(3), 293-301.
14. Mahomoodally, M. F., Dall’Acqua, S., Sinan, K. I., Sut, S., Ferrarese, I., Etienne, O. K. and Zengin, G. (2020). Phenolic compounds analysis of three Euphorbia species by LC-DAD-MSn and their biological properties. Journal of pharmaceutical and biomedical analysis, 189, 113477.
15. Mehmood, S., Syed, F., Khan, M. I., & Aadil, R. M. (2021). Bioactivity evaluation and phytochemical screening of Euphorbia helioscopia and Rumex dentatus. Food Science and Technology, 42, e66721.
16. Rafiq, S., Qadir, S., Wani, I. H., Ganie, S. A., Masood, A., & Hamid, R. (2014). Purification and partial characterization of a fructose-binding lectin from the leaves of Euphorbia helioscopia. Pak. J. Pharm. Sci, 27(6), 1805-1810.
17. Saleem, U., Saleem, M., Ahmad, B., Hussain, K., Ahmad, M., Bukhari, N. I., & Anjum, A. A. (2015). In-vitro antimicrobial susceptibility testing of leaves methanol extract and latex of Euphorbia helioscopia using agar well diffusion and broth dilution methods. The Journal of Animal and Plant Sciences. 25(1):261-267.
18. Singh, M., Rani, A., & Gupta, D. K. (2018). Euphorbia helioscopia L: past, present and future prospects. Bulletin of Pure & Applied Sciences-Botany. 37(1), 20-26.
19. Singh, M., Rani, A., & Gupta, D. K. (2023). Euphorbia helioscopia: Past, Present and Future Prospects. Advances and Challenges in Science and Technology. 84.
20. Valgas, C., Souza, S. M. D., Smânia, E. F., & Smânia Jr, A. (2007). Screening methods to determine antibacterial activity of natural products. Brazilian journal of microbiology, 38, 369-380.
21. Waheed, K., Muhammad, S. K., Shomaila, A., Muhammad, Z., Izhar, U., & Ullah, S. (2020). Antimicrobial activity and phytochemical screening of Euphorbia helioscopia. Planta daninha, 38, e020213727.
22. Wang, W. P., Jiang, K., Zhang, P., Shen, K. K., Qu, S. J., Yu, X. P. and Tan, C. H. (2018). Highly oxygenated and structurally diverse diterpenoids from Euphorbia helioscopia. Phytochemistry, 145, 93-102.
23. Yang, Y., Chen, X., Luan, F., Wang, M., Wang, Z., Wang, J., & He, X. (2021). Euphorbia helioscopia L.: A phytochemical and pharmacological overview. Phytochemistry. 184, 112649.
24. Zhu, Q., Jiang, M. L., Shao, F., Ma, G. Q., Shi, Q., & Liu, R. H. (2020). Chemical composition and antimicrobial activity of the essential oil from Euphorbia helioscopia L. Natural Product Communications. 15(9), 1934578X20953249.

Most read articles by the same author(s)