IDENTIFICATION OF ΒETA-GALACTOSIDASE PRODUCING BACTERIA FROM SOIL SAMPLES AND ENZYME OPTIMIZATION
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Abstract
β Galactosidase are hydrolases that can be found in microorganisms like fungi, bacteria, and yeasts, as well as plants, animal cells, and recombinant sources. The enzyme is used for two purposes: eliminating lactose from milk products for lactose intolerant persons and creating galactosylated goods. This study aimed to isolate and optimize beta-galactosidase-producing microbe from a soil sample collected near a dairy farm. For screening X-gal (5-bromo-4-chloro-3-indoyl-β-d-galactopyranoside), a chromogenic substrate essential as an indicator of glycosidase activity by giving it a blue colour, is used. Maximum production of enzyme was obtained with pHat7 and temperature at37ºC.Other factors of maximum production were observed in sucrose, ammonium sulphate, magnesiumsulphate and wheat flour. In enzyme assay ONPG (Ortho-Nitrophenyl-β-galactoside) was used as substrate. These results reveal a Lactobacillus spp. producing β-galactosidase obtained from soil samples with favorable characteristics has a vital role in foodindustries.
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Dr Farheen Aslam
Lahore College for Women University
Maria Akhtar
The University of Lahore
Dr Saiqa Ilyas
Lahore College for Women University
Dr Kiran
Lahore College for Women University
Dr Amna Tariq
Lahore College for Women University
Hajra Sohail
University of Central Punjab
Usman Minhas
Pakistan Council of Scientific and Industrial Research (PCSIR)
Dr Syed Zeeshan Haider Naqvi
The University of Lahore