ROLE OF ROS AND ANTIOXIDANT BIOMARKERS AFTER CHEMOTHERAPY IN PANCREATIC CARCINOMA AND ITS COMPARISON WITH PHARMACOTHERAPY
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Abstract
Introduction: Pancreatic carcinoma remains one of the most challenging malignancies to treat, characterized by its aggressive nature and limited response to conventional therapies.
Objective: The main objective of the study is to find the role of ROS and antioxidant biomarkers after chemotherapy in pancreatic carcinoma and its comparison with pharmacotherapy. Methodology of the study: This prospective observational study was conducted at Rashid Latif Medical College, Lahore during June 2022 to June 2023. A total of 220 adult patients diagnosed with pancreatic carcinoma were included in the study. Demographic and clinical data, including age, gender, tumor stage, histological subtype, treatment regimen, and treatment history, were collected from medical records. Baseline laboratory assessments, such as complete blood count, liver function tests, and serum tumor markers, were also recorded.
Results: Among the 220 patients enrolled in the study and the mean age was 56.76 ± 8.01 years. The most common histological subtype was adenocarcinoma (85%), and the majority of patients presented with advanced-stage disease (stage III/IV, 75%). The most frequently administered chemotherapy regimen was gemcitabine-based (65%), followed by FOLFIRINOX (25%) and nab-paclitaxel plus gemcitabine (10%). There were slight increases in Superoxide Dismutase (SOD) and Catalase levels after pharmacotherapy compared to chemotherapy (SOD: 115 ± 14 U/mg protein vs. 110 ± 12 U/mg protein, p = 0.07; Catalase: 90 ± 11 U/mg protein vs. 85 ± 10 U/mg protein, p = 0.04), the differences were not statistically significant. However, Glutathione levels were significantly higher after pharmacotherapy compared to chemotherapy (2.5 ± 0.7 µmol/g Hb vs. 2.2 ± 0.6 µmol/g Hb, p = 0.05). Vitamin C levels showed a significant increase after pharmacotherapy compared to chemotherapy (11 ± 1.8 µmol/L vs. 9 ± 1.5 µmol/L, p < 0.01).
Conclusion: It is concluded that monitoring and modulating ROS and antioxidant biomarkers play a crucial role in improving treatment responses and survival outcomes in pancreatic carcinoma patients. Pharmacotherapy, particularly strategies enhancing antioxidant defenses, shows superior efficacy compared to chemotherapy alone.
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Khadeeja Khan
Manchester Foundation Trust UK
Dr Eman Shaukat
House Officer, Aziz Fatimah Trust Hospital, Faisalabad
Sarwat Jahan
Associate Professor Department of Pharmacology and Therapeutics, Aziz Fatimah Medical and Dental College, Faisalabad
Dr Ambreen Nawaz
Assistant Professor General Pathology, Azra Naheed Dental College, Superior University, Lahore.
Dr Maryam Raza
Assistant Professor Physiology Department, Rashid Latif Medical College, Lahore
Dr Rao Salman Aziz
Associate Professor Pharmacology department Rashid Latif Medical & Dental College Lahore
Dr Amal Shaukat
Assistant Professor, Department of Food Science and Technology, University of Central Punjab
Faiqa Chaudhry
Lecturer, Department of Allied Health Sciences, University of Sargodha, Pakistan
Kanza Saleem
Master Student, Institute of Quality & Technology Management, Punjab University, Lahore