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Asim Ullah
Hamda Azmat
Fayyaz Rasool
Muhammad Tayyab


Carbofuran, Labeo rohita, mitigation, Zn NPs


The present study was conducted in order to explore the mitigation efficiency of biologically synthesized Zn NPs against carbofuran toxicity in Labeo rohita. For this purpose black pepper leaves extract was used Zn NPs were synthesized.  Initially 96 hrs LC50 for fish fingerlings against carbofuran was determined which was 0.3mg/l. Then 90 days trial was conducted to study the chronic effects of carbofuran in Labeo rohita fingerlings and its mitigation through biologically synthesized Zn NPs as supplemented feed. .  A total 180 healthy individuals of similar weight and length 30±05 of Labeo rohita were maintained and acclimatized prior to the experiment.  Fish were randomly distributed into three groups designated as T1, T2 and T3. Treatment 1 was given no carbofuran and was considered as control group while T2 and T3 were received 1/5th and 1/10th carbofuran sublethal concentrations of 96-hr LC50 (0.3 mg/l) respectively. Each treatment was be divided into four into four groups, each having fish (n=15). Four levels of supplemented feed i.e, 0 mg/kg, 5 mg/kg, 10 mg/kg and 15 mg/kg Zn BNPs (Biologically synthesized) were given to each group of the three treatments. Among the four feed levels 10mg/kg Zn NPs containing feed was found best in order to mitigated the chronic effects carbofuran by analyzing liver biomarkers and antioxidant enzyme assays.

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