TARAXACUM OFFICINALE AND CIRSIUM ARVENSE METHANOLIC EXTRACTS AMELIORATE OXIDATIVE STRESS AND LIPID PROFILE IN HIGH FAT DIET-INDUCED HYPERCHOLESTEROLEMIC RATS
Main Article Content
Keywords
Taraxacum officinale, Cirsium arvense, Hypercholesterolemia, Antioxidant activity, Paraoxonase activity
Abstract
The present study was designed to evaluate the effect of Taraxacum officinale and Cirsium arvense to ameliorate oxidative stress and lipid profile. For this purpose, methanol extract of the Taraxacum officinale and Cirsium arvense was prepared. Total phenol and total flavonoid content of the extract was performed. In vitro, the antioxidant activity of the plants was evaluated by ABTS, FRAP and DPPH assay. Then, 6 weeks old 40 albino rats were taken for in vivo study. Rats were divided into 5 groups; control group (CON), hypercholesterolemic group and Saline solution (HC+Saline), hypercholesterolemia and Atorvastatin (Hc+As), hypercholesterolemia and Cirsium arvense (Hc+Cs) and hypercholesterolemia and Taraxacum officinale (HC+To). Chow maintenance diet was served to the control group throughout the experiment. All the groups were fed on high-fat diet and treatments for 42 days. At the end of the experiment, animals were slaughtered to collect blood and serum for analysis of oxidative stress indicators, lipid profile and liver enzyme levels. Results showed the presence of TPC and TFC in both plants Taraxacum officinale and Cirsium arvense. Methanol extracts also showed antioxidant activity in ABTS, FRAP and DPPH assay. In experimental model, results showed that total antioxidant capacity (TAC), Paraoxonase activity, arylesterase activity, catalase, and SOD were significantly increased in Hc+To and Hc+Cs group as compared to the other groups. TOS and MDA were significantly decreased in treatment Hc+To and Hc+Cs group as compared to the other groups. Total cholesterol, LDL and triglyceride levels was also reduced in Hc+To and Hc+Cs group as compared to the other groups. The extract improved the HDL level in Hc+To and Hc+Cs groups. The study concluded that Taraxacum officinale and Cirsium arvense has the ability to improve the oxidative stress and lipid profile.
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Zonaira Nisar
Department of Eastern medicine, Government College University Faisalabad, Pakistan
Syed Muhammad Ali Shah
Department of Eastern medicine, Government College University Faisalabad, Pakistan
Sultan Ayaz
Department of Eastern medicine, Government College University Faisalabad, Pakistan
Abid Rashid
Faculty of Medical Sciences, Government College University Faisalabad, Pakistan
Imtiaz Mustafa
Institute of Molecular Biology and Biotechnology, The University of Lahore, Pakistan