EFFICACY OF A POLYHERBAL FORMULATION IN ALLEVIATING SEIZURES IN A RAT MODEL OF EPILEPSY

Main Article Content

Taufiq Ahmad
Sultan Ayaz
Abid Rashid
Hafiz Muhammad Asif
Seyd Muhammad Ali Shah

Keywords

Epilepsy, Polyherbal extract, Catharanthus roseus, Ocimum sanctum, Valeriana officinalis, Withania somnifera

Abstract

Epilepsy poses a significant global health burden, necessitating the exploration of alternative therapeutic approaches. This research investigates the antiepileptic and neuropharmacological potential of a polyherbal extract comprising Catharanthus roseus (CR), Ocimum sanctum (OS), Valeriana officinalis (VO), and Withania somnifera (WS) in a rat model. The study employs behavioral assays, forebrain biogenic amine level assessments, and neuropharmacological tests to comprehensively evaluate the effects of the polyherbal extract. Leg extension time, stupor time, flexion time, and righting reflex recovery time are among the seizure-related metrics that significantly decreased in the polyherbal extract and diazepam-treated groups as compared to the control group, indicating the antiepileptic activity. Serotonin, dopamine, and noradrenaline levels significantly rise following the injection of polyherbal extract, according to forebrain biogenic amine analysis, indicating a possible regulation of neurotransmitter systems. Increased squares crossed in the Open Field Test, increased rearing behaviour, decreased cage crossing activity, longer travel times in the Traction Test, and longer periods of immobility in the Forced Swimming Test are all indicators of anxiolytic and sedative effects revealed by neuropharmacological evaluations. These results are consistent with the antioxidant, neuroprotective, and anxiolytic qualities seen in each of the polyherbal formulation's constituent ingredients. This research sets the stage for the exploration of phytotherapeutic alternatives in epilepsy treatment, emphasizing the potential of natural remedies in neurological disorder management.

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References

1. Acar, S., Kapucu, A., & Akgün-Dar, K. (2022). The effects of regular swimming exercise during sodium valproate treatment on seizure behaviors and EEG recordings in pentylenetetrazole-kindled rats. Epilepsy Research, 179, 106830.
2. Aderibigbe, A. (2016). Neurobehavioral and Anticonvulsant effects of ethanol extract of Albizia adianthifolia leaves in experimental animals. Archives of Basic and Applied Medicine, 4(2), 77–85-77–85.
3. Adiga, U., & Nandit, P. (2023). An Overview of Pathogenesis of Epilepsy. International Neurourology Journal, 27(4), 88-94.
4. Ahmed, M., Sahibzada, M. U. K., Rasheed, H. M., Khan, T., Wahid, F., Farooq, U., Khusro, A., Uddin, J., Afzal, S., & Khan, A. (2022). Inhibition of Inflammation Associated Corneal Neovascularization by Dalbergia sissoo and Catharanthus roseus Leaf Extracts in an Animal Model. South African Journal of Botany, 151, 379-386.
5. Al-Attraqchi, O. H., Deb, P. K., & Al-Attraqchi, N. H. A. (2020). Review of the Phytochemistry and Pharmacological Properties of Valeriana officinalis. Current Traditional Medicine, 6(4), 260-277.
6. Amuri, B., Maseho, M., Simbi, L., Okusa, P., Duez, P., & Byanga, K. (2017). Hypoglycemic and antihyperglycemic activities of nine medicinal herbs used as antidiabetic in the region of Lubumbashi (DR Congo). Phytotherapy research, 31(7), 1029-1033.
7. Bashir, A., Nabi, M., Tabassum, N., Afzal, S., & Ayoub, M. (2023). An updated review on phytochemistry and molecular targets of Withania somnifera (L.) Dunal (Ashwagandha). Frontiers in Pharmacology, 14, 1049334.
8. Behl, T., Sharma, A., Sharma, L., Sehgal, A., Zengin, G., Brata, R., Fratila, O., & Bungau, S. (2020). Exploring the multifaceted therapeutic potential of withaferin A and its derivatives. Biomedicines, 8(12), 571.
9. Beppe, G. J., Dongmo, A. B., Foyet, H. S., Dimo, T., Mihasan, M., & Hritcu, L. (2015). The aqueous extract of Albizia adianthifolia leaves attenuates 6-hydroxydopamine-induced anxiety, depression and oxidative stress in rat amygdala. BMC Complementary and Alternative Medicine, 15(1), 1-13.
10. Bhattacharya, A., Ramanathan, M., Ghosal, S., & Bhattacharya, S. (2000). Effect of Withania somnifera glycowithanolides on iron‐induced hepatotoxicity in rats. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 14(7), 568-570.
11. Boota, M., Shah, S. M. A., Rashid, A., Akram, M., Ayaz, S., Mustafa, I., Nisar, J., & Nisar, Z. (2022). The Hepatoprotective and Anti-Nephrotoxic Potential of Methanolic Extract of a Polyherbal Preparation in CCl4-Induced Liver Injury Model of Wistar Rats. Dose-Response, 20(3), 15593258221124728.
12. Das, G., Shin, H.-S., Tundis, R., Gonçalves, S., Tantengco, O. A. G., Campos, M. G., Acquaviva, R., Malfa, G. A., Romano, A., & Robles, J. A. H. (2021). Plant species of sub-family Valerianaceae—A review on its effect on the central nervous system. Plants, 10(5), 846.
13. Dossi, E., & Huberfeld, G. (2023). GABAergic circuits drive focal seizures. Neurobiology of Disease, 106102.
14. Eldeen, I., Elgorashi, E., & Van Staden, J. (2005). Antibacterial, anti-inflammatory, anti-cholinesterase and mutagenic effects of extracts obtained from some trees used in South African traditional medicine. Journal of Ethnopharmacology, 102(3), 457-464.
15. Guerrini, R., Conti, V., Mantegazza, M., Balestrini, S., Galanopoulou, A. S., & Benfenati, F. (2023). Developmental and epileptic encephalopathies: from genetic heterogeneity to phenotypic continuum. Physiological Reviews, 103(1), 433-513.
16. Gupta, S. K., Dua, A., & Vohra, B. P. (2003). Withania somnifera (Ashwagandha) attenuates antioxidant defense in aged spinal cord and inhibits copper induced lipid peroxidation and protein oxidative modifications. Drug metabolism and drug interactions, 19(3), 211-222.
17. Ha, J. W., Yu, J. S., Lee, B. S., Kang, D.-M., Ahn, M.-J., Kim, J. K., & Kim, K. H. (2022). Structural Characterization of Withanolide Glycosides from the Roots of Withania somnifera and Their Potential Biological Activities. Plants, 11(6), 767.
18. Han, K., Jin, X., Guo, X., Cao, G., Tian, S., Song, Y., Zuo, Y., Yu, P., Gao, G., & Chang, Y.-Z. (2021). Nrf2 knockout altered brain iron deposition and mitigated age-related motor dysfunction in aging mice. Free Radical Biology and Medicine, 162, 592-602.
19. Krabbe, S., Duda, J., Schiemann, J., Poetschke, C., Schneider, G., Kandel, E. R., Liss, B., Roeper, J., & Simpson, E. H. (2015). Increased dopamine D2 receptor activity in the striatum alters the firing pattern of dopamine neurons in the ventral tegmental area. Proceedings of the National Academy of Sciences, 112(12), E1498-E1506.
20. Łukasiuk, K., & Lasoń, W. (2023). Emerging Molecular Targets for Anti-Epileptogenic and Epilepsy Modifying Drugs. International Journal of Molecular Sciences, 24(3), 2928.
21. Marcucci, C., Rademacher, M., Kamecki, F., Pastore, V., Bach, H. G., Ricco, R. A., Wagner, M. L., Knez, D., Gobec, S., & Colettis, N. (2023). Biological Evaluation of Valeriana Extracts from Argentina with Potent Cholinesterase Inhibition for the Treatment of Neurodegenerative Disorders and Their Comorbidities—The Case of Valeriana carnosa Sm.(Caprifoliaceae) Studied in Mice. Pharmaceuticals, 16(1), 129.
22. Meftahi, G. H., Hatef, B., & Jahromi, G. P. (2023). Creatine Activity as a Neuromodulator in the Central Nervous System. Archives of Razi Institute, 78(4).
23. Moon, S. G., Kim, J.-K., Lee, S.-Y., & Kim, H. K. (2023). Trends of Epilepsy-Related Mortality in South Korea. Journal of Clinical Neurology (Seoul, Korea), 19(2), 174.
24. Mustafa, I., Faisal, M. N., Hussain, G., Muzaffar, H., Imran, M., Ijaz, M. U., Sohail, M. U., Iftikhar, A., Shaukat, A., & Anwar, H. (2021). Efficacy of Euphorbia helioscopia in context to a possible connection between antioxidant and antidiabetic activities: a comparative study of different extracts. BMC Complementary Medicine and Therapies, 21, 1-12.
25. Nisar, J., Ali Shah, S. M., Ayaz, S., Akram, M., Rashid, A., Mustafa, I., & Nisar, Z. (2023). In vitro comparative evaluation of Tamarix gallica extracts for antioxidant and antidiabetic activity. Experimental Biology and Medicine, 248(3), 253-262.
26. Nkwingwa, B. K., Wado, E. K., Foyet, H. S., Bouvourne, P., Jugha, V. T., Mambou, A. H. M. Y., Bila, R. B., & Taiwe, G. S. (2023). Ameliorative effects of Albizia adianthifolia aqueous extract against pentylenetetrazole-induced epilepsy and associated memory loss in mice: Role of GABAergic, antioxidant defense and anti-inflammatory systems. Biomedicine & Pharmacotherapy, 165, 115093.
27. Panigrahi, A. K., Satapathy, S., Mishra, S. K., & Jena, S. K. (2022). Effect of Ocimum sanctum leaf extract in animal models of anxiety. National Journal of Physiology, Pharmacy and Pharmacology, 12(7), 1084-1084.
28. Pinzon, R. T., Buwana, F., Sanyasi, R. D. L. R., Dharmawan, A., & Wijono, J. (2019). Antiepileptic drugs and quality of life in patients with epilepsy: A tertiary care hospital-based study. Asian Journal of Pharmacy and Pharmacology, 5(2), 249-254.
29. Sarris, J., Panossian, A., Schweitzer, I., Stough, C., & Scholey, A. (2011). Herbal medicine for depression, anxiety and insomnia: a review of psychopharmacology and clinical evidence. European neuropsychopharmacology, 21(12), 841-860.
30. Speers, A. B., Cabey, K. A., Soumyanath, A., & Wright, K. M. (2021). Effects of Withania somnifera (Ashwagandha) on stress and the stress-related neuropsychiatric disorders anxiety, depression, and insomnia. Current neuropharmacology, 19(9), 1468.
31. Stafford, R. S. (2008). Regulating off-label drug use—rethinking the role of the FDA. New England Journal of Medicine, 358(14), 1427-1429.
32. Tolambiya, P., Jagetiya, B., & Mathur, S. (2023). EXTRACTION AND IDENTIFICATION OF VARIOUS PHYTOPHARMACEUTICAL CONSTITUENT OF CATHARANTHUS ROSEUS L.(ALBA) LEAVES. Plant Archives, 23(2), 158-165.
33. Yuniarti, W. M., Krismaharani, N., Ciptaningsih, P., Celia, K., Veteriananta, K. D., Ma’ruf, A., & Lukiswanto, B. S. (2021). The protective effect of Ocimum sanctum leaf extract against lead acetate-induced nephrotoxicity and hepatotoxicity in mice (Mus musculus). Veterinary World, 14(1), 250.

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