EXTRACTION, CHARACTERIZATION, ANTIBACTERIAL AND ANALGESIC ACTIVITY OF ESSENTIAL OILS OBTAINED FROM MICROCEPHALA LAMELLATA AND ALHAGI MAURORUM
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Keywords
Microcephala lamellata, Alhagi maurorum, Essential oil, Phytochemical analysis, FTIR, Gas chromatography
Abstract
The primary and secondary metabolites of medicinal plants possess pharmacological activities such as anti-bacterial, anthelmintic, analgesic, anti-rheumatic etc. The current study evaluated essential oils of Microcephala lamellata (ML) and Alhagi maurorum (AM) for their primary and secondary metabolites, antibacterial and analgesic activities. The phytochemical screening of essential oils of both plants showed the presences of phenolic compounds, terpenes and terpenoids. The thin layer chromatographic studies with different mobile phases separated different compounds in both oils. The gas chromatographic studies also confirmed the presences of phenolic compounds terpenes and terpenoids. Analysis by UV-visible spectroscopy showed the absorption maxima at 230-235nm and 280-285nm, respectively. The FTIR analysis, indicated the presence of functional groups such as phenols (OH stretching vibrations), alkanes (CH stretching vibrations), carboxylic acid (C = O carboxylic acid stretching vibrations), methyl (CH3 bending vibrations), and carbonyl (C-O stretching vibrations). The antibacterial studies as evaluated by disc diffusion method have revealed that AM essential oils have antibacterial activity against B. subtillis, E.coli, P. aeruinosa and S. aureus whereas the ML essential oils have low antibacterial activity against these microbial strains. Both essential oils showed significant analgesic activity in the dose dependent manner as evaluated by acetic acid induced writhing test.
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Amjad Hussain
Department of Eastern Medicine, Government college, University Faisalabad-Pakistan
sultan ayaz
Department of Eastern Medicine, Government college, University Faisalabad-Pakistan
Abid Rashid
Department of Eastern Medicine, Government college, University Faisalabad-Pakistan
Syed Muhammad Ali Shah
Department of Eastern Medicine, Government college, University Faisalabad-Pakistan
Muhammad Akram
Department of Eastern Medicine, Government college, University Faisalabad-Pakistan