FREQUENCY AND ANTIMICROBIAL SUSCEPTIBILITY PROFILES OF URINARY PATHOGENS

Main Article Content

Dr. Sadia Ikram
Dr Saima Inam
Dr Tariq Ahmed
Dr Ayesha Sarwar
Dr Nida Javed
Ifza Shahzadi
Dr Syed Zeeshan Haider Naqvi
Dr Aroosh Shabir
Dr Ashok Kumar

Keywords

UTI, Nitrofurantoin, ESBL, E.coli

Abstract

Urinary tract infections are among the commonly occurring microbial infections ranked as second frequently occurring infectious disease with 150 million diagnosed cases per year. (1) This study was intended to oversee the occurrence of pathogens isolated from urine cultures along with the anti-microbial susceptibility patterns of most common pathogen obtained in a tertiary care Centre. A retrospective study was conducted in the Microbiology department of Chaudhary Mohammad Akram Research and Training Hospital (CMA), Lahore during the period of March 2021 to December 2021. Urine cultures received during this 10 month period were analyzed and the most common organism isolated along with its susceptibility pattern was assessed. During study period, 200 urine cultures were received, out of which 93 turned out to be positive, making the percentage positivity of 46.5%. Most common organism was E.coli i.e. 43%, followed by Candida species i.e. (17%). E.coli was found in 52.5% of female population. Considering the susceptibility pattern of E.coil, most sensitive options of antibiotics were Nitrofurantoin (87.5%), followed by Imipenem (70%) & Amikicin (62.5%). Most resistant antibiotic options were Ampicillin, Nalidixic acid & Ciprofloxacin (2.5%). 82.5% of E.coli isolates were found to be ESBL producers. This research concluded that patients with UTI are at high risk of developing antibiotic resistance. Uro-pathogens isolated in the present study exhibited highest rate of susceptibility against nitrofurantoin, amikacin and imipenem which can be empirically utilized for treatment of urinary tract infections.

Abstract 135 | pdf Downloads 68

References

1. Akram M, Shahid M, Khan AU. Etiology and antibiotic resistance patterns of community-acquired urinary tract infections in JNMC Hospital Aligarh, India. Annals of clinical microbiology and antimicrobials. 2007;6(1):1-7.
3. Stamm WE, Norrby SR. Urinary tract infections: disease panorama and challenges. The Journal of infectious diseases. 2001;183(Supplement_1):S1-S4.
4. Cunha MA, Assunção GLM, Medeiros IM, Freitas MR. Antibiotic resistance patterns of urinary tract infections in a northeastern Brazilian capital. Revista do Instituto de Medicina Tropical de São Paulo. 2016;58.
5. Haque R, Akter ML, Salam MA. Prevalence and susceptibility of uropathogens: a recent report from a teaching hospital in Bangladesh. BMC research notes. 2015;8(1):1-5.
6. Brooks G, Butel J, Morse S. Enteric gram-negative rods (Enterobacteriaceae). Jawetz, Melnick, & Adelberg’s Medical Microbiology 23rd ed USA: McGraw-Hill. 2004:248-61.
7. Schaeffer A, Rajan N, Cao Q, Anderson B, Pruden DL, Sensibar J, et al. Host pathogenesis in urinary tract infections. International journal of antimicrobial agents. 2001;17(4):245-51.
8. Stamm WE. Urinary tract infections and pyelonephritis. Harrisons Principles of Internal Medicine. 2005;16(2):1715.
9. Spellberg B, Bartlett JG, Gilbert DN. The future of antibiotics and resistance. New England Journal of Medicine. 2013;368(4):299-302.
10. McQuiston Haslund J, Rosborg Dinesen M, Sternhagen Nielsen AB, Llor C, Bjerrum L. Different recommendations for empiric first-choice antibiotic treatment of uncomplicated urinary tract infections in Europe. Scandinavian journal of primary health care. 2013;31(4):235-40.
11. Humayun T, Iqbal A. The culture and sensitivity pattern of urinary tract infections in females of reproductive age group. Ann Pak Inst Med Sci. 2012;8(1):19-22.
12. Odoki M, Almustapha Aliero A, Tibyangye J, Nyabayo Maniga J, Wampande E, Drago Kato C, et al. Prevalence of bacterial urinary tract infections and associated factors among patients attending hospitals in Bushenyi district, Uganda. International journal of microbiology. 2019;2019.
13. Serretiello E, Folliero V, Santella B, Giordano G, Santoro M, De Caro F, et al. Trend of Bacterial Uropathogens and Their Susceptibility Pattern: Study of Single Academic High-Volume Center in Italy (2015–2019). International journal of microbiology. 2021;2021.
14. Pouladfar G, Basiratnia M, Anvarinejad M, Abbasi P, Amirmoezi F, Zare S. The antibiotic susceptibility patterns of uropathogens among children with urinary tract infection in Shiraz. Medicine. 2017;96(37).
15. Rezaee MA, Abdinia B. Etiology and antimicrobial susceptibility pattern of pathogenic bacteria in children subjected to UTI: a referral hospital-based study in northwest of Iran. Medicine. 2015;94(39).
16. Amraei S, Karouei SMH, Babakhani S, Kazemi MJ. Serotyping and antibiotic susceptibility pattern of common bacterial uropathogens in urinary tract infections in koohdasht, lorestan province. International Journal of Enteric Pathogens. 2016;4(2):9-34824.
17. Yang B, Yang F, Wang S, Wang Q, Liu Z, Feng W, et al. Analysis of the spectrum and antibiotic resistance of uropathogens in outpatients at a tertiary hospital. Journal of Chemotherapy. 2018;30(3):145-9.
18. Folliero V, Caputo P, Della Rocca MT, Chianese A, Galdiero M, Iovene MR, et al. Prevalence and antimicrobial susceptibility patterns of bacterial pathogens in urinary tract infections in University Hospital of Campania “Luigi Vanvitelli” between 2017 and 2018. Antibiotics. 2020;9(5):215.
19. Obiofu EN, Ige OH, Iroro O. Antimicrobial susceptibility pattern of urinary isolates from outpatients suspected for urinary tract infection. GSC Biological and Pharmaceutical Sciences. 2018;5(3):-.
20. Khan FB, Shahzad K, Khan NB, Kokab Z, Iqbal Z, Mansoor K. Common uropathogens and their antimicrobial susceptibility patterns at a tertiary care hospital in Pakistan. Journal of Rawalpindi Medical College. 2020;24(4):306-10.
21. Jamil J, Haroon M, Sultan A, Khan MA, Gul N, Kalsoom U. Prevalence, antibiotic sensitivity and phenotypic screening of ESBL/MBL producer E. coli strains isolated from urine; District Swabi, KP. Pakistan J Pak Med Assoc. 2018;68(11):1704-7.
22. Guevara N, Guzman M, Merentes A, Rizzi A, Papaptzikos J, Rivero N, et al. Antimicrobial susceptibility patterns of Gram-negative bacteria isolated in urinary tract infections in Venezuela: Results of the SMART study 2009-2012. Revista chilena de infectologia: organo oficial de la Sociedad Chilena de Infectologia. 2015;32(6):639-48.

Most read articles by the same author(s)