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Sidra Zubair
Safila Naveed
Sana Sarfarz
Hira Afshan
Subia Jamil
Hira Raees
Asma Eraj
Mahrukh khurshid


Anxiolytic, coconut milk, pineapple juice, cherry juice


Anxiety is an emotion of uneasiness, fear or discomfort. It causes restlessness and difficulty in focus. Recently due to the high costs of allopathic medicines and increasing adverse effects, the trend has switched to exploring nutrients in a normal diet that can be therapeutically beneficial. Cherry, pineapple and coconut milk have a great impact on CNS activity because they contain different flavonoids and other phytochemicals. This study was designed to assess the anxiolytic and memory-boosting potential of a cocktail of cherry juice, pineapple juice and coconut milk. This study was performed on albino mice weighing 18-22gm of both sexes. Mice were divided into 2 groups. Group I was taken as Control and was given normal saline 0.4ml. Group II was taken as treated and given a cocktail of 0.4ml. Head dip, light and dark model, cage crossing, open field and elevated plus maze were used to evaluate effects on anxiety. A stationary rod test was used to evaluate memory-boosting effects. Our study showed significant anxiolytic activities (p ≤ 0.05) of cocktails in mice. Head dip activity, light and dark activity, cage crossing activity, and elevated plus maze show highly significant (p≤ 0.001) results on anxiety. Open field activity showed significant (p ≤0.05) results on anxiety. Stationary rod activity showed highly significant (p ≤ 0.001) results on the memory-boosting effect. It is concluded that the administration of a cocktail has anxiolytic and positive effects on memory because it possesses anti-oxidant components such as multivitamins, minerals and flavonoids. It has fewer adverse effects as compared to allopathic drugs.

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1. Stein, M.B. and J. Sareen, Generalized anxiety disorder. New England Journal of Medicine, 2015. 373(21): p. 2059-2068.
2. Sarris, J., et al., Complementary medicine, exercise, meditation, diet, and lifestyle modification for anxiety disorders: a review of current evidence. Evidence-Based Complementary and Alternative Medicine, 2012. 2012.
3. Arcos-Carmona, I., et al., [Effects of aerobic exercise program and relaxation techniques on anxiety, quality of sleep, depression, and quality of life in patients with fibromyalgia: a randomized controlled trial]. Medicina clínica, 2011. 137(9): p. 398-401.
4. Herring, M.P., K.E. Johnson, and P.J. O'Connor, Exercise training and health-related quality of life in generalized anxiety disorder. Psychology of sport and exercise, 2016. 27: p. 138-141.
5. Bandelow, B., S. Michaelis, and D. Wedekind, Treatment of anxiety disorders. Dialogues in clinical neuroscience, 2017. 19(2): p. 93.
6. Neha, K., et al., Medicinal prospects of antioxidants: A review. European journal of medicinal chemistry, 2019. 178: p. 687-704.
7. Lv, Y.-W., et al., Advanced in studies on anxiolytic effects of natural flavonoids. Zhongguo Zhong yao za zhi= Zhongguo zhongyao zazhi= China journal of Chinese materia medica, 2016. 41(1): p. 38-44.
8. Wallace, T.C. and M.M. Giusti, Anthocyanins. Advances in Nutrition, 2015. 6(5): p. 620-622.
9. Mirto, A., et al., Metabolic characterization and antioxidant activity in sweet cherry (Prunus avium L.) Campania accessions: Metabolic characterization of sweet cherry accessions. Food chemistry, 2018. 240: p. 559-566.
10. Wali, N., Pineapple (Ananas comosus), in Nonvitamin and nonmineral nutritional supplements. 2019, Elsevier. p. 367-373.
11. Lima, E., et al., Cocos nucifera (L.)(Arecaceae): A phytochemical and pharmacological review. Brazilian Journal of Medical and Biological Research, 2015. 48(11): p. 953-964.
12. Souza, L., et al., Lithium and valproate prevent methylphenidate-induced mania-like behaviors in the hole board test. Neuroscience letters, 2016. 629: p. 143-148.
13. Qureshi, M., et al., Evaluation of neuropharmacological, analgesic and anti-inflammatory effects of the extract of Centella asiatica (Gotu kola) in mice. African Journal of Pharmacy and Pharmacology, 2015. 9(41): p. 995-1001.
14. Lucon-Xiccato, T., G. De Russi, and C. Bertolucci, A novel-odour exploration test for measuring anxiety in adult and larval zebrafish. Journal of Neuroscience Methods, 2020. 335: p. 108619.
15. Zewde, A.M., et al., PLDT (planarian light/dark test): an invertebrate assay to quantify defensive responding and study anxiety-like effects. Journal of neuroscience methods, 2018. 293: p. 284-288.
16. Okonogi, T., et al., Characterization of peripheral activity states and cortical local field potentials of mice in an elevated plus maze test. Frontiers in Behavioral Neuroscience, 2018. 12: p. 62.
17. Costa, A. and D. Herrero, Perspectives on the use of mouse models of down syndrome in translational research involving visual and motor functions. Journal of Human Growth and Development, 2014. 24(2): p. 123-125.
18. Najam, R. and B. Riaz, Neuropharmacological screening of zamzam water revealed its anxiolytic and antidepressant effect. International Journal of Therapeutic Applications, 2016. 32: p. 41-47.
19. Solangi, M.A., ANXIOLYTIC AND MEMORY ENHANCING ACTIVITY OF L. SICERARIA IN RODENTS. International Journal of Biomedical and Advance Research, 2013. 4(1): p. 40-46.
20. Kishore, R.N., et al., Evaluation of anxiolytic activity of ethanolic extract of Foeniculum vulgare in mice model. Int J Pharm Pharm Sci, 2012. 4(3): p. 584-586.
21. Marcinkiewcz, C.A., et al., Serotonin engages an anxiety and fear-promoting circuit in the extended amygdala. Nature, 2016. 537(7618): p. 97-101.
22. Pastorino, G., et al., Liquorice (Glycyrrhiza glabra): A phytochemical and pharmacological review. Phytotherapy research, 2018. 32(12): p. 2323-2339.
23. Veasey, R.C., et al., The effects of supplementation with a vitamin and mineral complex with guaraná prior to fasted exercise on affect, exertion, cognitive performance, and substrate metabolism: a randomized controlled trial. Nutrients, 2015. 7(8): p. 6109-6127.
24. Khoo, H.E., et al., Phytochemicals and medicinal properties of indigenous tropical fruits with potential for commercial development. Evidence-Based Complementary and Alternative Medicine, 2016. 2016.
25. Foster, S., Tyler's honest herbal: a sensible guide to the use of herbs and related remedies. 2012: Routledge.
26. Opdenakker, G., et al., Applications of glycobiology: biological and immunological effects of a chemically modified amylose-derivative. Carbohydr. Chem, 2012. 38: p. 1-12.
27. Xie, W., et al., Panax notoginseng saponins: a review of its mechanisms of antidepressant or anxiolytic effects and network analysis on phytochemistry and pharmacology. Molecules, 2018. 23(4): p. 940.
28. Hamid, H.A., A.N. Ramli, and M.M. Yusoff, Indole alkaloids from plants as potential leads for antidepressant drugs: A mini review. Frontiers in pharmacology, 2017. 8: p. 96.
29. Nakamura, M., et al., Low zinc, copper, and manganese intake is associated with depression and anxiety symptoms in the Japanese working population: Findings from the eating habit and well-being study. Nutrients, 2019. 11(4): p. 847.