Assessment of stem cells growth characteristics ethyl gallate conjugated hydrogel
Main Article Content
Keywords
stem cell, Gallic acid, ethyl gallate, scaffold
Abstract
Background: Stem cells are types of cells that can produce other cells which are able to develop into any kind of cells in the body. scaffolds are 3-D porous solid, that provides a spatial correct position of cell location .They hold enormous potential for tissue regeneration. Ethyl gallate exhibits non-toxic nature of cell line from different tissue origin ethyl Gallate also provides nutrition and has been proven.
Materials and methods: 20 ML of gelatin solution was taken and mixed or added with 20 MG of Gallic acid(ethyl Gallate). It is then poured into a dish (90 MM Patriplate). freezing it was done at 4°C for 12 hours in a 24L culture plate. Thin membrane is now fabricated and characterized by SEM and an anti-inflammatory test was also done.
Discussion: Gallic acid-based hydrogel was found to have a stimulatory effect on stem cell proliferation .Improved porosity of the hydrogel is also seen, to facilitate proliferation.
Conclusion: Gallic acid(Ethyl gallate) modified gelatin membranes seem to exhibit significant effect on stem cell growth, characteristics which can be effectively extrapolated to key regeneration strategies.
References
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22. Menon A, Thenmozhi MS. Correlation between thyroid function and obesity. J Adv Pharm Technol Res. 2016 Oct;9(10):1568.
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24. Priyanka S, Kaarthikeyan G, Nadathur JD, Mohanraj A, Kavarthapu A. Detection of cytomegalovirus, Epstein-Barr virus, and Torque Teno virus in subgingival and atheromatous plaques of cardiac patients with chronic periodontitis. J Indian Soc Periodontol. 2017 Nov;21(6):456–60.
25. Babu S, Jayaraman S. An update on β-sitosterol: A potential herbal nutraceutical for diabetic management. Biomed Pharmacother. 2020 Nov;131:110702.
26. Gopalakannan S, Senthilvelan T, Ranganathan S. Modeling and Optimization of EDM Process Parameters on Machining of Al 7075-B4C MMC Using RSM. Procedia Engineering. 2012 Jan 1;38:685–90.
27. Sahu D, Kannan GM, Vijayaraghavan R. Size-dependent effect of zinc oxide on toxicity and inflammatory potential of human monocytes. J Toxicol Environ Health A. 2014;77(4):177–91.
28. Wang Y, Zhang Y, Guo Y, Lu J, Veeraraghavan VP, Mohan SK, et al. Synthesis of Zinc oxide nanoparticles from Marsdenia tenacissima inhibits the cell proliferation and induces apoptosis in laryngeal cancer cells (Hep-2). J Photochem Photobiol B. 2019 Dec;201:111624.
2. Seetharaman R, Mahmood A, Kshatriya P, Patel D, Srivastava A. An Overview on Stem Cells in Tissue Regeneration. Curr Pharm Des. 25(18):2086–98.
3. Takezawa T. A strategy for the development of tissue engineering scaffolds that regulate cell behavior [Internet]. Vol. 24, Biomaterials. 2003. p. 2267–75. Available from: http://dx.doi.org/10.1016/s0142-9612(03)00038-3
4. Krishna L, Dhamodaran K, Jayadev C, Chatterjee K, Shetty R, Khora SS, et al. Nanostructured scaffold as a determinant of stem cell fate. Stem Cell Res Ther. 2016 Dec 30;7(1):1–12.
5. A multi-functional scaffold for tissue regeneration: The need to engineer a tissue analogue. Biomaterials. 2007 Dec 1;28(34):5093–9.
6. Development of a modular reinforced bone tissue engineering scaffold with enhanced mechanical properties. Mater Lett. 2022 Jul 1;318:132170.
7. Honda Y, Takeda Y, Li P, Huang A, Sasayama S, Hara E, et al. Epigallocatechin Gallate-Modified Gelatin Sponges Treated by Vacuum Heating as a Novel Scaffold for Bone Tissue Engineering. Molecules. 2018 Apr 11;23(4):876.
8. Kasper C, van Griensven M, Pörtner R. Bioreactor Systems for Tissue Engineering II: Strategies for the Expansion and Directed Differentiation of Stem Cells. Springer Science & Business Media; 2010. 331 p.
9. The effect of scaffold architecture on odontogenic differentiation of human dental pulp stem cells. Biomaterials. 2011 Nov 1;32(31):7822–30.
10. Ma H, Hu J, Ma PX. Polymer scaffolds for small-diameter vascular tissue engineering. Adv Funct Mater [Internet]. 2010 Sep 9 [cited 2023 May 7];20(17). Available from: https://pubmed.ncbi.nlm.nih.gov/24501590/
11. Odontoblasts: the cells forming and maintaining dentine. Int J Biochem Cell Biol. 2004 Aug 1;36(8):1367–73.
12. Rung S, Zhao X, Chu C, Yang R, Qu Y, Man Y. Application of Epigallocatechin-3-gallate (EGCG) Modified 1-Ethyl-3-(3-dimethylaminopropylcarbodiimide hydrochloride/N-hydroxy-succinimide (EDC/NHS) Cross-Linked Collagen Membrane to Promote Macrophage Adhesion. Materials . 2021 Aug 18;14(16):4660.
13. Mohan S, Thiagarajan K, Chandrasekaran R. In vitro evaluation of antiproliferative effect of ethyl gallate against human oral squamous carcinoma cell line KB. Nat Prod Res [Internet]. 2015 [cited 2023 May 7];29(4). Available from: https://pubmed.ncbi.nlm.nih.gov/25104086/
14. Kalaivani T, Rajasekaran C, Mathew L. Free radical scavenging, cytotoxic, and hemolytic activities of an active antioxidant compound ethyl gallate from leaves of Acacia nilotica (L.) Wild. Ex. Delile subsp. indica (Benth.) Brenan. J Food Sci [Internet]. 2011 Aug [cited 2023 May 7];76(6). Available from: https://pubmed.ncbi.nlm.nih.gov/22417526/
15. Zhang Z, Gupte MJ, Ma PX. Biomaterials and stem cells for tissue engineering. Expert Opin Biol Ther [Internet]. 2013 Mar 11 [cited 2023 May 7]; Available from: https://www.tandfonline.com/doi/abs/10.1517/14712598.2013.756468
16. Saraiva SM, Miguel SP, Ribeiro MP, Coutinho P, Correia IJ. Synthesis and characterization of a photocrosslinkable chitosan–gelatin hydrogel aimed for tissue regeneration [Internet]. Vol. 5, RSC Advances. 2015. p. 63478–88. Available from: http://dx.doi.org/10.1039/c5ra10638a
17. Tsai CC, Young TH, Chen GS, Cheng NC. Developing a Glyoxal-Crosslinked Chitosan/Gelatin Hydrogel for Sustained Release of Human Platelet Lysate to Promote Tissue Regeneration [Internet]. Vol. 22, International Journal of Molecular Sciences. 2021. p. 6451. Available from: http://dx.doi.org/10.3390/ijms22126451
18. Li J, Wang W, Li M, Song P, Lei H, Gui X, et al. Biomimetic Methacrylated Gelatin Hydrogel Loaded With Bone Marrow Mesenchymal Stem Cells for Bone Tissue Regeneration [Internet]. Vol. 9, Frontiers in Bioengineering and Biotechnology. 2021. Available from: http://dx.doi.org/10.3389/fbioe.2021.770049
19. Neelakantan P, Subbarao C, Subbarao CV, De-Deus G, Zehnder M. The impact of root dentine conditioning on sealing ability and push-out bond strength of an epoxy resin root canal sealer. Int Endod J. 2011 Jun;44(6):491–8.
20. Thejeswar EP, Thenmozhi MS. Educational research-iPad system vs textbook system. J Adv Pharm Technol Res. 2015;8(8):1158.
21. Saravanan A, Senthil Kumar P, Jeevanantham S, Karishma S, Tajsabreen B, Yaashikaa PR, et al. Effective water/wastewater treatment methodologies for toxic pollutants removal: Processes and applications towards sustainable development. Chemosphere. 2021 Oct;280:130595.
22. Menon A, Thenmozhi MS. Correlation between thyroid function and obesity. J Adv Pharm Technol Res. 2016 Oct;9(10):1568.
23. Mohanavel V, Ashraff Ali KS, Prasath S, Sathish T, Ravichandran M. Microstructural and tribological characteristics of AA6351/Si3N4 composites manufactured by stir casting. Journal of Materials Research and Technology. 2020 Nov 1;9(6):14662–72.
24. Priyanka S, Kaarthikeyan G, Nadathur JD, Mohanraj A, Kavarthapu A. Detection of cytomegalovirus, Epstein-Barr virus, and Torque Teno virus in subgingival and atheromatous plaques of cardiac patients with chronic periodontitis. J Indian Soc Periodontol. 2017 Nov;21(6):456–60.
25. Babu S, Jayaraman S. An update on β-sitosterol: A potential herbal nutraceutical for diabetic management. Biomed Pharmacother. 2020 Nov;131:110702.
26. Gopalakannan S, Senthilvelan T, Ranganathan S. Modeling and Optimization of EDM Process Parameters on Machining of Al 7075-B4C MMC Using RSM. Procedia Engineering. 2012 Jan 1;38:685–90.
27. Sahu D, Kannan GM, Vijayaraghavan R. Size-dependent effect of zinc oxide on toxicity and inflammatory potential of human monocytes. J Toxicol Environ Health A. 2014;77(4):177–91.
28. Wang Y, Zhang Y, Guo Y, Lu J, Veeraraghavan VP, Mohan SK, et al. Synthesis of Zinc oxide nanoparticles from Marsdenia tenacissima inhibits the cell proliferation and induces apoptosis in laryngeal cancer cells (Hep-2). J Photochem Photobiol B. 2019 Dec;201:111624.
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Published
Jun 24, 2023
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How to Cite
Muskan Soni, Ramya Ramadoss, Raghunandakumar, P.Bargavi, Sandhya Sundar, Suganya Panneer Selvam, & Pratibha Ramani. (2023). Assessment of stem cells growth characteristics ethyl gallate conjugated hydrogel. Journal of Population Therapeutics and Clinical Pharmacology, 30(10), 536–540. https://doi.org/10.47750/jptcp.2023.30.10.058
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