Comparative diagnostic analysis, frequency, severity, and seroprevalence of Dengue fever

Sameer Zafar; Samia Yousaf; Maria Irshad Chohan; Shah Zeb; Mohammed Garout; Muhammad Zahid Ali; Muhammad A. Halwani; Mohammed Aljeldah; Ahlam Alynbiawi; Saleh A. Alshamrani; Sarah A. Algosaibi; Roua A. Alsubki; Abdulmonem A. Alsaleh; Ali A. Rabaan

doi:10.47750/jptcp.2023.30.15.049

Sameer Zafar

Department of Medical Education, Sharif Medical and Dental College, Lahore, Punjab, Pakistan

Samia Yousaf

Department of General Medicine, St Mary’s Hospital, Isle of Wight NHS trust, UK

Maria Irshad Chohan

Department of Medical Education, Sheikh Zayed Medical College, Rahim Yar Khan, Punjab, Pakistan

Shah Zeb

Department of Microbiology, Pakistan Kidney and Liver Institute and Research Center, Lahore, Punjab, Pakistan

Mohammed Garout

Department of Community Medicine and Health Care for Pilgrims, Faculty of Medicine, Umm Al-Qura University, Makkah 21955, Saudi Arabia

Muhammad Zahid Ali

Department of Microbiology, Yahya Welfare Complex Hospital, Haripur.

Muhammad A. Halwani

Department of Medical Microbiology, Faculty of Medicine, Al Baha University. Al Baha 4781, Saudi Arabia

Mohammed Aljeldah

Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, University of Hafr Al Batin, Hafr Al Batin 39831, Saudi Arabia

Ahlam Alynbiawi

Infectious Diseases Section, Medical Specialties Department, King Fahad Medical City, Riyadh 12231, Saudi Arabia

Saleh A. Alshamrani

Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Najran University, Najran 61441, Saudi Arabia

Sarah A. Algosaibi

Academic and clinical Training, Eastern Health Cluster-Rural health network, Dammam 32247, Saudi Arabia

Roua A. Alsubki

Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11362, Saudi Arabia

Abdulmonem A. Alsaleh

Department of Clinical Laboratory Sciences, Mohammed AlMana College of Health Sciences, Dammam 34222, Saudi Arabia

Ali A. Rabaan

Molecular Diagnostic Laboratory, Johns Hopkins Aramco Healthcare, Dhahran 31311, Saudi Arabia

Keywords

Dengue Fever, Virus serotypes, diagnostics

Abstract

The study involved a total of 450 participants, with 359 males and 91 females. Out of a larger group of 4820 individuals, 4170 tested positive for dengue, while 650 tested negative. In the subsequent analysis, the negative cases were excluded, added only the positive cases. Signs and symptoms of dengue were analyzed, including fever (p = 0.007), rapid and weak pulse, pallor or cool skin, rashes (p = 0.005), and headache (p = 0.001). The mean difference between genders was 2085, with a standard deviation of 771 and a variance of 594441.The 95% confidence interval was 2,085 ± 1,068.551 (±51.25%), and the p-value was 0.002. The mean differences for specific markers related to dengue were as follows: IgM = 548.5, IgG = 294.5, NS1 = 949, and IgG/IgM = 293. The standard deviations for these markers were IgM = 71.5, IgG = 23.5, NS1 = 738, and IgG/IgM = 128. The 95% confidence intervals were calculated as 1.960σx̄, 521.25 ± 320.176 (±61.42%), with a variance of V = 213479.75 and a p-value of 0.009. The overall mean difference for dengue serotypes was 521.2, with a standard deviation of 350.4 and a variance of 122845.18. The 95% confidence interval was 521.25 ± 242.879 (±46.60%), with a p-value of 0.007. The mean±SD values for hemoglobin = 16.0±2.41, platelets = 71250.2±24321.5, and hematocrit = 47.3±7.22. In dengue hemorrhagic fever, the mean±SD values were hemoglobin = 17.2±2.83, platelets = 32412.5±27530.2, and hematocrit = 66.5±9.72. The study highlighted the endemic nature and seroprevalence of dengue within the vicinity of the civil hospital. The highest seroprevalence of dengue, indicated by the NS1 marker, was observed in males, while the lowest prevalence was seen in females, possibly due to more males working in tire shops and labor-intensive jobs.

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Published

Jun 22, 2023

DOI https://doi.org/10.47750/jptcp.2023.30.15.049

How to Cite

Sameer Zafar, Samia Yousaf, Maria Irshad Chohan, Shah Zeb, Mohammed Garout, Muhammad Zahid Ali, Muhammad A. Halwani, Mohammed Aljeldah, Ahlam Alynbiawi, Saleh A. Alshamrani, Sarah A. Algosaibi, Roua A. Alsubki, Abdulmonem A. Alsaleh, & Ali A. Rabaan. (2023). Comparative diagnostic analysis, frequency, severity, and seroprevalence of Dengue fever. Journal of Population Therapeutics and Clinical Pharmacology, 30(15), 425–433. https://doi.org/10.47750/jptcp.2023.30.15.049