GENETIC CHARACTERIZATION OF MITOCHONDRIAL DNA CONTROL REGION OF MUGHAL ETHNIC GROUP IN PAKISTAN
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Keywords
Mitochondrial DNA (mtDNA), Control Region Sequencing, Mughal Population, Genetic Diversity, Maternal Lineages, Haplotypes, Population Genetics, Forensic Genetics, South Asian Haplogroups.
Abstract
Studying populations at the genetic level is crucial for identifying population-specific trends. Numerous fields, including forensic science and health, use population-specific genetic patterns for investigation and analysis. A given ethnic group's maternal lineages, as well as its evolutionary and emigrational histories, can be gleaned from the examination of mtDNA control regions. The given ethnic group's maternal lineages, as well as its evolutionary and emigrational histories, can be obtained from the examination of mtDNA control regions. No such work has been reported on this ethnic group and for studying the genetic variability this study was designed. Therefore, in this study, the mitochondrial DNA haplotypes of the Mughal population from Pakistan's CR (control region) from 16024 base pairs to 576 base pairs are described.
To examine the genetic variance caused by maternally inherited DNA in this community, 100 unrelated samples of Mughals from upper Pakistan had their mtDNA control region sequences compared to the rCRS (revised Cambridge reference sequence). Out of a total of 91 haplotypes, the results showed that 88 were found to be unique. This population shared only 3 haplotypes. Nearly 62% of the haplotypes belonged to South Asian haplogroups, with evidence of Northren Asian and Northren European haplogroups (about 7%) also present, indicating admixed maternal genetics in this community.
The Mughal population has a high level of genetic diversity (0.999), the lowest rate of random matches (0.0100), and a high level of discriminatory capacity (0.9900). This study states the presence of different people from different regions of the world which refers to scientifically describe the origin and the settlement of these people from all over the world.
References
2. Anderson, S., A. T. Bankier, B. G. Barrell, M. H. L. De Bruijn, A. R. Coulson, and J. Drouin. 1981. Sequence and organization of the human mitochondrial genome. Nature. 290:457-465.
3. Andrew, R. M., I. Kubacka, P. F. Chinnery, R. N. Lightowlers, D. M. Turnbull, N. Howell. Reanalysis and revision of the Cambridge reference sequence for human mitochondrial DNA. Nat Genet 1999; 23:147.
4. Ayub, Q., M. Massimo, L. Pagani, M. Haber, A. Mohyuddin and K. Shagufta. 2015. The Kalash genetic isolate: ancient divergence, drift, and selection. Am. J. Hum. Genet. 96(5):775-783.
5. Barbieri, C., M. Vicente, S. Oliveira, K. Bostoen, J. Rocha and M. Stoneking. 2014. Migration and interaction in a contact zone: mtDNA variation among Bantu-speakers in southern Africa. PloS one. 9(6): 99117.
6. Pilipenko, A. S., Trapezov, R. O., Cherdantsev, S. V., Babenko, V. N., Nesterova, M. S., Pozdnyakov, D. V., ... & Polosmak, N. V. (2018). Maternal genetic features of the Iron Age Tagar population from Southern Siberia (1st millennium BC). PLoS One, 13(9), e0204062.
7. Barfield, T. (2023). Afghanistan: A cultural and political history.
8. Cano, D., C. F. Gomez, N. Ospina, J. A. Cajigas, H. Groot and R. E. Andrade. 2014. Mitochondrial DNA haplogroups and susceptibility to prostate cancer in a colombian population. ISRN oncol.
9. Fontana, M. 2011. Matteo Ricci. A Jesuit in the Ming Court. Rowm. Lil. Fie. Pub. 32.
10. Goto, H., B. Dickins, E. Afgan, I. M. Paul, J. Taylor, K. D. Makova and A. Nekrutenko. 2011. Dynamics of mitochondrial heteroplasmy in three families investigated via a repeatable re-sequencing study. Genome biol.12(6):59.
11. Hayat, S., T. Akhtar, M. H. Siddiqi, A. Rakha, N. Haider and M. Tayyab. 2015. Mitochondrial DNA control region sequences study in Saraiki population from Pakistan. Legal Medicine. 17(2): 140-144.
12. Hoseinzadeh, S. J., N. Fazeli, Z. Hassan, Montazer, Mostafa and S. Zare. 2016. Ancient DNA Analysis of Goat Bones in Kashan and Qazvin plain in the Neolithic Period. J. Archaeological Stu.7(2):33-45.
13. Van Schendel, W. (2020). A history of Bangladesh. Cambridge University Press.
14. Jafari, M., & Ansari-Pour, N. (2019). Why, when and how to adjust your P values?. Cell Journal (Yakhteh), 20(4), 604.
15. Lehninger, A. L., L. D. Nelson and M. C. Michael. 2000. Lehninger principles of biochemistry. New York: Worth Publishers.
16. Ma, J., C. Coarfa, X. Qin, P. E. Bonnen, A. Milosavljevic, J. Versalovic and K. Aagaard. 2014. mtDNA haplogroup and single nucleotide polymorphisms structure human microbiome communities. BMC genom. 15(1):257.
17. Metspalu, M., Kivisild, T., Metspalu, E., Parik, J., Hudjashov, G., Kaldma, K., ... & Villems, R. (2004). Most of the extant mtDNA boundaries in south and southwest Asia were likely shaped during the initial settlement of Eurasia by anatomically modern humans. BMC genetics, 5(1), 1-25.
18. Nesheva, D. V. (2014). Aspects of ancient mitochondrial DNA analysis in different populations for understanding human evolution. Balkan journal of medical genetics, 17(1), 5-14.
19. Rakha, A., K. J. Shin, J. A. Yoon, N. Y. Kim, M. H. Siddique and I. S. Yang. 2011. Forensic and genetic characterization of mtDNA from Pathans of Pakistan. Int. J. Leg. Med. 125(6):841-848.
20. Siddiqi, M. H., T. Akhtar, A. Rakha, G. Abbas, A. Ali and N. Haider. 2015. Genetic characterization of the Makrani people of Pakistan from mitochondrial DNA control-region data. Leg. Med. 17(2):134-139.
21. Fisher, M. C., & Garner, T. W. (2020). Chytrid fungi and global amphibian declines. Nature Reviews Microbiology, 18(6), 332-343.
22. Ullah, I., H. Ahmad, B. E. Hemphill, M. S. Nadeem, M. Tariq and S. Tabassum. 2017. Mitochondrial genetic characterization of Gujar population living in the Northwest areas of Pakistan. Advancements Lif. Sci. 4(3): 84-91.
23. Vieira-Machado, C. D., M. Tostes, G. Alves, J. Nazer, L. Martinez, and E. Wettig. 2016. Uniparental ancestry markers in Chilean populations. Genet. Mol. Biol. 39(4):573-579.
24. Whale, J. W. (2012). Mitochondrial DNA analysis of four ethnic groups of Afghanistan (Doctoral dissertation, University of Portsmouth).
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All articles published in JPTCP are licensed under Copyright Creative Commons Attribution-NonCommercial 4.0 International License.
Farah Ahmad
Department of Biosciences and Technology, Emerson University, Multan.
Department of Basic and Applied Chemistry, University of Central Punjab Lahore, Pakistan.
Muhammad Afzal
Department of Basic and Applied Chemistry, University of Central Punjab Lahore, Pakistan.
Muhammad Saqib Shahzad
Government Post Graduate College Model Town Lahore, Pakistan.
Muhammad Aleem Ashraf
Department of Biosciences and Technology, Emerson University, Multan.