COMPARATIVE ANALYSIS OF ALZHEIMER'S DISEASES: IDENTIFICATION OF HUBS TO FORECAST DIAGNOSIS THROUGH COMPREHENSIVE DATA MINING
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Keywords
Neurodegeneration, Transcriptomics, Hub genes, Alzheimer’s, Parkinson’s, Bioinformatics
Abstract
Alzheimer's disease (AD) and Parkinson's disease (PD) are the most common neurodegenerative disorders, often sharing overlapping molecular mechanisms. This study seeks to identify common differentially expressed genes (DEGs) and hub genes between AD and PD using gene expression data from publicly available datasets. We analyzed the GSE122063 dataset for AD and GSE20141 dataset for PD, both sourced from the Gene Expression Omnibus (GEO). After preprocessing, normalization, and differential expression analysis, common DEGs were identified and a protein-protein interaction (PPI) network was constructed using the STRING database. Hub genes were determined using the cytoHubba plugin in Cytoscape based on various centrality measures. The key findings of this study reveal shared pathways involving cellular stress response, neuroinflammation, and synaptic dysfunction. Significant hub genes, including APP, SNCA, MAPT, and TP53, may represent novel therapeutic targets for both AD and PD. These results contribute to understanding the shared molecular landscape between these neurodegenerative disorders and open new avenues for the development of therapeutic interventions.
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Ahmad Hayat
Department of Zoology, the Islamia University of Bahawalpur, Pakistan
Muhammad Afzal
Department of Biochemistry, Faisalabad Medical University, Faisalabad, Pakistan
Shazia Anwer Bukhari
Department of Biochemistry, Government College University Faisalabad, Pakistan
Muhammad Ahmad Majeed
Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan
Abdullah Sethar
Livestock Breeding Services Authority (LBSA) Sindh, Livestock & Fisheries Department, Government of Sindh, Pakistan
Nabila Farah
IMBB (Institute of Molecular Biology and Biotechnology), The University of Lahore, Lahore, Pakistan
Atiqa Saleem
Department of Biomedical Engineering, HITEC University Taxila, Pakistan
Ayesha Hassan
Department of Biosciences, COMSATS University Islamabad, Park Rd, Islamabad Capital Territory 45550, Pakistan
Syed Sabir Ali Shah
Livestock and Fisheries department, Government of Sindh, Pakistan
Tamseel Saleem
Department of Physiology and Biochemistry, Faculty of Animal Husbandry and Veterinary Sciences (AHVS), Sindh Agriculture University, Tandojam, Pakistan
Akbar Ali
Nishtar Medical University, Pakistan