DIFFERENTIAL EXPRESSION OF PREDICTIVE VARIABLES OF MEDICAL IMPORTANCE AND THEIR INTERPLAY TO DEVELOP RHEUMATOID ARTHRITIS

Sheikh Khurram Salam Sehgal; Hina Allah Ditta; Asia Hussain; Tariq Hussain; Rihana Dilshad; Amina Jawaid; Arif Malik; Ayesha Zahid

doi:10.53555/jptcp.v31i6.6554

Sheikh Khurram Salam Sehgal
Hina Allah Ditta
Asia Hussain
Tariq Hussain
Rihana Dilshad
Amina Jawaid
Arif Malik
Ayesha Zahid

Keywords

Rheumatoid Arthritis, Autoimmune Disease, MDA, 8-OHdG, 4-HNE

Abstract

INTRODUCTION: Rheumatoid arthritis (RA) is an inflammatory disorder that may be characterized by an autoimmune multisystem disease. Other important characteristic features include inflammation of synovial membrane of joints followed by the pain and swelling. Primary aim of the current study is to elucidate the effects of various predictive variables in the development of the rheumatoid arthritis and their differential expression in blood, saliva and synovial fluid of the subjects.

MATERIALS AND METHODS: For the current study fifty (n=50) patients of clinically diagnosed (RA) along with fifty (n=50) age and sex matched individuals were included. blood, saliva and synovial fluid samples were collected from Jinnah hospital Lahore and were stored at -70^օC until assayed. All of the experimental protocols were approved by research ethical committee of Institute of Molecular biology and Biotechnology (IMBB) University of Lahore. Levels of Malondialdehyde (MDA), Isoprostanes, 8-Hydroxy-2-Deoxyguanosine (8-OHdG) and 4-Hydroxynonenal (4-HNE) were estimated by their respective spectrophotometric and ELISA methods.

RESULTS: Findings of the study stated elevated levels of the said markers in the diseased group as compared to the healthy individuals. Levels remained sensitive in the synovial fluid as compared to the serum and saliva. MDA levels remained elevated in serum, saliva and synovial fluids in subjects (0.95±0.019, 0.056±0.0056, 0.019±0.0016) as compared to the controls (1.95±0.094, 0.012±0.0034, 3.26±0.65). Similarly, the levels of Isoprostanes, 8-OHdG and 4-HNE also remained higher in the subjects when compared in controls.

CONCLUSION: Findings of the study show that levels of the said markers remained higher among the patients of rheumatoid arthritis when compared with the healthy controls. Findings show that the levels remained more sensitive in the synovial fluid as compared to the serum and saliva samples thus, it may be concluded that estimation of the said variables in the synovial fluid may remain as promising variable as the indicator of early onset of the rheumatoid arthritis.

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Published

Jun 9, 2024

DOI https://doi.org/10.53555/jptcp.v31i6.6554

How to Cite

Sheikh Khurram Salam Sehgal, Hina Allah Ditta, Asia Hussain, Tariq Hussain, Rihana Dilshad, Amina Jawaid, Arif Malik, & Ayesha Zahid. (2024). DIFFERENTIAL EXPRESSION OF PREDICTIVE VARIABLES OF MEDICAL IMPORTANCE AND THEIR INTERPLAY TO DEVELOP RHEUMATOID ARTHRITIS. Journal of Population Therapeutics and Clinical Pharmacology, 31(6), 694–701. https://doi.org/10.53555/jptcp.v31i6.6554

Issue

Vol. 31 No. 6 (2024): JPTCP

Section

Articles

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