TO STUDY THE SEROPREVALENCE OF HEPATITIS-C VIRUS INFECTION AMONG PATIENTS UNDERGOING SURGERY AT A TERTIARY CARE HOSPITAL IN KANPUR
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Keywords
Sero-prevalence, Hepatitis C Virus, Surgery, Rapid Card, RT-PCR.
Abstract
Background: Hepatitis C virus (HCV) is among the major causes of acute and chronic hepatitis that leads to hepatic cirrhosis and carcinoma. According to global estimates, the prevalence rate of HCV in India is continuously increased in past decades and contributed around more than 11 million patients. Routine sensitive and specific methods are needed so that precise diagnosis can be made especially in patients undergoing surgical interventions. The present work compared the sensitivity and specificity of the rapid card test versus real time PCR (RT-PCR) for HCV confirmation in patients undergoing surgical procedure in several departments.
Aim: The aims of the present study are to compare the Sensitivity and Specificity of the Rapid card test versus RT-PCR in detection of HCV and also determine the sero-prevalence of HCV in the study region.
Methods: A cross-sectional study was conducted in the Department of Microbiology and Department of Medicine RMCH&RC UP from April 2023 to May 2024.All patients undergoing surgery were included in this study. Rapid HCV card test along with RT-PCR were performed to screen patients prior to surgery, and for each patient who tested positive using the rapid HCV, qualitative RT-PCR testing was done to confirm active viral load. Comparison of sensitivity and specificity were made between RAPID card test and RT-PCR.
Results: The sero-prevalence of HCV in this study was 2%, among 2400 patients screened for HCV infection by using Rapid card & RT-PCR tests. 45 samples were found positive with Rapid Card test. While 48 sample were found positive by RT-PCR. Males were more affected than females. Sensitivity and specificity observed for rapid card was 85.29% and 100%, and RT-PCR showed100% sensitivity and specificity.
Conclusion: The study results concluded that RT-PCR method is more sensitive and specific compared to rapid card test for the detection of HCV and should be performed in all positive cases to confirm and to know the viral load of HCV.
References
2. WHO: Hepatitis B. Fact Sheet No.204. World Health Organization. WHO: Hepatitis B. Fact Sheet_B_CTC.cdr 2016
3. World Health Organization, “Hepatitis B,” Fact Sheet 204, World Health Organization, Geneva, Switzerland, 2015.
4. M. J. U. Dahoma, A. A. Salim, R. Abdool et al., “HIV and substance abuse: the dual epidemics challenging Zanzibar,” African Journal of Drug & Alcohol Studies2006; 5 (2): 131–139..
5. R. M. Merrill and B. D. Hunter et al. “Sero-prevalence of markers for hepatitis B viral infection,” International Journal of Infectious Diseases 2011; 15 (2):78–e12.
6. Vranckx R, AlisjahbanaA, MeheusA et al. Hepatitis B virus vaccination and antenatal transmission of HBV markers to neonates. JViral Hepat 1999; 6:135-9.
7. Burnett R, Francois G, Kew G, et al. Hepatitis B virus and human immunodeficiency virus co-infection in sub-Saharan Africa: a call for further action. Liver Int 2005; 25:201-213.
8. Yang HI, Lu SN, Liaw YF, You SL, Sun CA, wang LY et al. Hepatitis B e antigen and the risk of hepatocellular carcinoma. N Engl J Med. 2002; 347: 168-174.
9. Maddrey WC. Hepatitis B: an important public health issue. J Med Virol. 2000;61:362-66.78
10. WHO Global Hepatitis Report. 2018.
11. Butsashvili et al. Barriers of linkage to HCV viremia testing among people who inject drugs in Georgia Substance Abuse Treatment, Prevention, and Policy 2022; 17:23 2-6
12. Substance Abuse Treatment, Prevention, and Policy (2022) 17:23 Chaudhary IA, Khan SA, Samiullah Should we do Hepatitis B and C screening on each Patient before Surgery: Analysis of 142cases. Pak J Med Sci 2005; 21: 278-280
13. Aitken C, Jeffries DJ (2001) Nosocomial spread of viral disease.Clin Microbiol Rev 14: 528-546.2001
14. MacIsaac M, Whitton B, Hubble A et al. A successful hepatitis C outreach service in a medically supervised injecting room: increased uptake of screening and high rates of treatment initiation. VH2021, Sydney 2021.
15. Ustianowski A WM, PaveyK , Harwood J, Bell S, Bennett J, O’Connell L , Smiles J, Hammond S, Horne M, Roberts D, Vilar J et al. Rapid test & treat programme successfully facilitating hepatitis C micro-elimination in a women's prison. J. Hepatol. 2020; S 793.
16. Davies LBH, Matthews G, Plant J, Edwards S, Thompson-Jones H, Edwards H, Hughes L, Kneath S, Richards C, Wilkes K et al. Micro-elimination of Hepatitis C in HMP, Swansea - first for a remand prison in the UK. European Association for the Study of the Liver (EASL).Viena, 2019.
17. Sood A, Suryaprasad A, Trickey A, Kanchi S, Midha V, Foster MA, Bennett E, Kamili S, Alvarez-Bognar F, Shadaker S, Surlikar V, Garg R, Mittal P, Sharma S, May MT, Vickerman P, Averhoff F. The burden of hepatitis C virus infection in Punjab, India: A population-based serosurvey. PLoS One. 2018;13(7):e0200461. doi: 10.1371/journal.pone.0200461.
18. Niu Z, Zhang P, Tong Y. Age and gender distribution of Hepatitis C virus prevalence and genotypes of individuals of physical examination in WuHan, Central China. Springerplus. 2016; 13; 5(1):1557. doi: 10.1186/s40064-016-3224-z.
19. Mohamed Z, Mbwambo J, Rwegasha J, et al. In-field evaluation of Xpert® HCV viral load Fingerstick assay in people who inject drugs in Tanzania. Liver Int 2020; 40:514- 21.
20. RajkumarManojkumar Singh et al., Evaluation of Three Diagnostic Assays for HCV. Journal of Clinical and Diagnostic Research. 2022 Jul, Vol-16(7): DC13-DC17.
21. Vázquez-Morón S, Ryan P, Ardizone-Jiménez B, Martín D, Troya J, Cuevas G, Valencia J, Jimenez-Sousa MA, Avellón A, Resino S. Evaluation of dried blood spot samples for screening of hepatitis C and human immunodeficiency virus in a real-world setting. Sci Rep. 2018 Jan 30; 8(1):1858.
22. Croom HA, Richards KM, Best SJ, Francis BH, Johnson EI, Dax EM, Wilson KM. Commercial enzyme immunoassay adapted for the detection of antibodies to hepatitis C virus in dried blood spots. J ClinVirol. 2006 May;36(1):68-71
23. Judd A, Parry J, Hickman M, McDonald T, Jordan L, Lewis K, Contreras M, Dusheiko G, Foster G, Gill N, Kemp K, Main J, Murray-Lyon I, Nelson M. Evaluation of a modified commercial assay in detecting antibody to hepatitis C virus in oral fluids and dried blood spots. J Med Virol. 2003 Sep; 71(1):49-55.
24. Poiteau L, Soulier A, Rosa I, Roudot-Thoraval F, Hézode C, Pawlotsky JM, Chevaliez S. Performance of rapid diagnostic tests for the detection of antibodies to hepatitis C virus in whole blood collected on dried blood spots. J Viral Hepat. 2016 May;23(5):399-401.
25. Nouhin J, Iwamoto M, Prak S, Dousset JP, Phon K, Heng S, et al. Molecular epidemiology of hepatitis C virus in Cambodia during 2016–2017. Sci Rep.2019;9(1):7314 10.1038/s41598-019-43785-4.
26. Maity S, Nandi S, Biswas S, Sadhukhan SK, Saha MK. Performance and diagnostic usefulness of commercially available enzyme linked immunosorbent assay and rapid kits for detection of HIV, HBV and HCV in India. Virol J. 2012; 9: 290-99.
27. Mane A, Sacks J, Sharma S, Singh H, Tejada-Strop A, Kamili S, et al. Evaluation of five rapid diagnostic tests for detection of antibodies to hepatitis C virus (HCV): A step towards scale-up of HCV screening efforts in India. 2019; 14(1):e0210556.
28. Chevaliez S, Roudot-Thoraval F, HezodeC,et al. Performance of rapid diagnostic tests for HCV infection in serum or plasma. Future Microbial. 2021; 16(10):713-19.
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Simmi Singh
PG Student, Dept of Microbiology, Rama Medical College Hospital and Research Centre, Mandhana Kanpur
R Sujatha
Professor and Head, Dept of Microbiology, Rama Medical College Hospital & Research Centre Kanpur.
Deepak Sameer
Tutor, Dept of Microbiology, Rama Medical College and Hospital & Research Centre Kanpur.
Khutija Sarah
Assistant Professor, Dept of Microbiology, Rama Medical College Hospital & Research Centre Kanpur.
Archana
Associate Professor Dept of Medicine, Rama Medical College Hospital & Research Centre Kanpur
Suneet Yadav
Associate Professor, Dept of Medicine, Rama Medical College Hospital & Research Centre Kanpur.