COMPARATIVE STUDY OF DETECTION OF DIARRHEAGENIC ESCHERICHIA COLI (EHEC) IN CHILDREN (LESS THAN 10 YEARS) BY STOOL CULTURE AND ANTIGEN DETECTION BY RAPID CARD AT A TERTIARY CARE HOSPITAL IN KANPUR
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Keywords
EHEC, Sorbitol MacConkey agar, Rapid card O157:H7
Abstract
Background: Escherichia coli is a major public health concern that causes diarrhoea. Diarrheagenic Escherichia coli (DEC), is one of the most common species causing gastrointestinal illnesses worldwide, and causes hemorrhagic colitis, diarrhoea, and haemolytic-uremic syndrome (HUS)in children.
Objective: A comparative study of detection of diarrheagenic Escherichia coli (EHEC) in children (less than 10 years) by stool culture and antigen detection by rapid card was done at a tertiary care hospital in Kanpur.
Methods: The present study is a cross sectional observational study conducted in Department of Microbiology and Paediatrics Rama Medical College Hospital and Research Centre between April 2023 to March 2024. 45 stool samples were collected in a sterile container. The sample was cultured on Sorbitol MacConkey agar (SMAC) and standard guidelines were followed for identification and AST was performed according to CLSI guidelines. The rapid card antigen detection was also done for the detection of diarrhogenic EHEC.
Results: Out of 45 stool samples studied 24 were male and 21 were female children. In the age group 1-2 years two were positive by rapid card E.coli O157:H7 and one were positive by SMAC. The sensitivity and specificity of Rapid card is 100% and 97.72% EHEC E.coli was 100% sensitive to only Meropenem, Ciprofloxacin, Imipenem, Gentamicin and resistant was 100% to Tobramycin, Amikacin, Amplicillin sulbactam.
Conclusion: In our study Rapid card E.coli O157:H7 is more effective method of diagnosing EHEC when compared to SMAC with higher sensitivity and specificity value. Hence Rapid card is rapid and relevant method for detection of EHEC E.coli.
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Mansi Srivastav
PG student, Department of Microbiology Rama Medical College and Hospital & Research Centre Kanpur.
Deepak Sameer
Tutor, Department of Microbiology Rama Medical College and Hospital & Research Centre Kanpur.
R. Sujatha
Professor and Head, Department of Microbiology Rama Medical College Hospital & Research Centre Kanpur.
Suneet Yadav
Associate Professor, Department of Microbiology Rama Medical College Hospital & Research Centre Kanpur.